Yerba Mate, derived from the leaves of tree, Ilex paraguariensis, is widely-used as a tea or an ingredient in formulated foods. The aim present study was to evaluate effects Mate extract on weight loss, obesity-related biochemical parameters, and diabetes high-fat diet-fed mice.To this end, by using vivo animal models dietary-induced obesity, we have made interesting observations that has ability decrease differentiation pre-adipocytes reduce accumulation lipids adipocytes, both which...

Homologous desensitization of D₁ dopamine receptors is thought to occur through their phosphorylation leading arrestin association which interdicts G protein coupling. In order identify the relevant domains receptor phosphorylation, and determine how this leads association, we created a series mutated constructs. one mutant, all serine/threonine residues within 3rd cytoplasmic domain were altered (3rdTOT). A second construct was in only three these serines (serines 256, 258, 259)...

ABSTRACT Ovine herpesvirus 2 (OvHV-2), the major causative agent of malignant catarrhal fever in ruminant species worldwide, has never been propagated vitro. Using real-time PCR, a striking, short-lived, peak viral DNA, ranging from 10 5 to over 8 copies/2 μg was detected nasal secretions 60.7% adolescent sheep ( n = 56) at some point during period 6 9 months age. In contrast, only about 18% adult 33) experienced shedding episode study period. The general pattern appearance DNA dramatic rise...

We used the yeast two-hybrid assay to identify novel proteins that interact with D 1 dopamine receptor. The third cytoplasmic loop (residues 217–273) of rat receptor was as bait clones encoding interacting from a brain cDNA library. This identified two C terminus neurofilament-M (NF-M) 782–846). NF-M clone did not loops 2 , 3 or 4 receptors, but showed weak interaction 5 Coexpression full-length in HEK-293 cells resulted >50% reduction binding accompanied by receptor-mediated cAMP...

A malignant catarrhal fever (MCF)–like disease was induced experimentally in 3 sheep after aerosol inoculation with ovine herpesvirus-2 (OvHV-2). Each of OvHV-2–negative nebulized 2 ml nasal secretions containing approximately 3.07 − 10 9 OvHV-2 DNA copies from a experiencing an intensive viral-shedding episode. Ovine became detectable by polymerase chain reaction the peripheral blood leukocytes all within days, and seroconverted between 6 8 days postinfection (PI). The developed clinical...

In this study, we examined a colony of 20 beagle dogs in laboratory animal facility. Mycoplasma was detected by consensus PCR assay 1 dog with respiratory and constitutional symptoms. None the other were affected. The euthanized necropsied. postmortem examinations, gray or plum-colored gross lesions found on lung, most commonly apical cardiac lobes. Some showed clear demarcation consolidation. Microscopic examination peribronchiolar lymphoid hyperplasia interstitial thickening, pathognomonic...

We have isolated mutants of Epstein-Barr virus (EBV) which carry a dominant selectable marker inserted into the third exon gene encoding two membrane proteins, TP1 and TP2 (or LMP2A LMP2B), are expressed in latently infected, growth-transformed B cells. One also acquired 260-bp deletion beginning first intron few base pairs from terminal repeats removing most second TP exon, including initial coding sequences TP2. These EBV transform human cells culture, transformed B-cell clones carrying...

We have used the yeast two-hybrid system to identify protein kinase C-zeta interacting (ZIP) as a novel for D(2) dopamine receptor (DAR). This interaction was identified by screening rat brain cDNA library using third intracellular loop of DAR bait. A partial-length encoding ZIP isolated and characterized specifically with DAR, but not loops other subtypes. Biochemical confirmation ZIP-D(2) obtained expressing full-length proteins in mammalian cells demonstrating that they could be...

Erectile dysfunction (ED) is a highly prevalent disorder that affects millions of men worldwide. ED now considered an early manifestation atherosclerosis, and consequently, precursor systemic vascular disease. This study was designed to investigate the effects male silkworm pupa powder (SWP) on levels nitric oxide synthase (NOS) expression, nitrite, glutathione (GSH); lipid peroxidation; libido; erectile response corpus cavernosum rat penis. We induced in animals by oral administration 20%...

Mycoplasmas are highly fastidious bacteria, difficult to culture and slow growing. Many species of mycoplasmas important pathogens that cause respiratory infection in laboratory animals known affect experimental results obtained with contaminated animals. The aim the present study was develop a sensitive specific assay for detection mycoplasma species. To this end, we developed polymerase chain reaction dot blot hybridization (PCR/DBH) detecting DNA evaluated it its sensitivity specificity....

Constipation is one of the most common functional digestive complaints worldwide. We investigated laxative effects figs (Ficus carica L) in a beagle model constipation induced by high protein diet and movement restriction. The experiments were consecutively conducted over 9 weeks divided into 3 periods each. All 15 beagles subjected to non-treatment (control) period, induction fig paste treatment period. administered (12 g/kg daily, gavage) for following 3-week period dogs. Segmental colonic...

Abstract Because of the diversity in human papillomavirus (HPV) distribution, according to population and region, detailed investigations HPV genotypes are important designing more effective vaccines for any given country. DNA oligonucleotide microarray was used investigate distribution among commercial sex workers. The prevalence Korean workers 47%, with HPV‐16 HPV‐51 as dominant genotypes. subtypes 148 comprised 70 one genotype, 42 two genotypes, 17 three 19 four or HPV‐40, most low‐risk...

Cronobacter sakazakii (C. sakazakii), formerly Enterobacter sakazakii, is an emerging pathogen associated with the ingestion of contaminated reconstituted formula that causes serious illnesses such as bacteremia, septicemia, necrotizing enterocolitis, meningitis and death in low-birth-weight preterm neonatal infants. The objective this study was to develop animal model for human C. infections. We acquired timed-pregnant ICR mice allowed them give birth naturally. On postnatal day 3.5, each...

The purpose of this study was to examine the antiobesity effects Monascus pilosus-fermented black soybean (F-BS) in C57BL/6 mice with high-fat diet (HFD)-induced obesity. F-BS (oral, 0.5 and 1.0 g/kg per body weight, twice day) ameliorated obesity by reducing liver weight increases, regulating blood glucose cholesterol levels fed a control or HFD oral administration for 12 weeks. suppressed growth epididymal, retroperitoneal, perirenal fat pads preventing increases adipocyte size. Moreover,...

As malfunction/absence of immune cells causes a variety immunosuppressive disorders and chemical synthetic drugs for curing these diseases have many adverse effects, vigorous studies are being conducted. The Acanthopanax family has been used as traditional medicines gastric ulcer, diabetes, etc. culinary materials in East-South Asia. In this study, the immunostimulating properties A. sessiliflorus were evaluated. increased not only splenocyte number but also immune-related cytokines such...

A number of Helicobacter species may confound experimental data because their association with disease progressing in various kinds laboratory animals. Screening is particularly desirable, they are prevalent commercial and research animal facilities. The aim the present study was to compare three diagnostic methods [e.g. stool antigen kit (HpSA), polymerase chain reaction (PCR) rapid urease test (RUT)] for identification spp. stools or gastric biopsy specimens collected from eight dogs...

A one-year-old female English Cocker Spaniel dog with idiopathic Horner's Syndrome is described.The specific clinical signs in this specimen were miosis, ptosis, enophthalmos, and prolapsed nictitans for 2 days following sudden onset.According to history taking, ophthalmic, neurological, radiological examination, the patient was diagnosed syndrome.Manual acupuncture treatment applied on local points two times days.The acupoints ST-4 (Di Chang) GB-1 (Tong Zi Liao).The day after initial...

Eimeria tenella and maxima are important pathogens causing intracellular protozoa infections in laboratory avian animals known to affect experimental results obtained from contaminated animals. This study aimed find a fast, sensitive, efficient protocol for the molecular identification of E. samples using chickens as DNA was extracted fecal collected polymerase chain reaction (PCR) analysis employed detect DNA. The target nucleic acid fragments were specifically amplified by PCR. Feces...

Anticoccidial effects of the Plantago asiatica extract (PAE) were evaluated in chickens following oral infection with Eimeria (E.) tenella. This study was conducted on 3-day-old (n=30). Those animals divided 3 groups; PAE 0.1% treated/infected (n=10), untreated/infected (n=10) and non-infected control (n=10). Chickens fed a standard diet supplemented or without for 1 week prior to E. tenella (10,000 sporulated oocysts per chicken). The assessed by two parameters; fecal shedding body weights...

An 8-year-old female Yorkshire Terrier was presented for investigation of reduced appetite, and occasional vomiting. She has been treated with medroxyprogesterone acetate (MPA) from past 3 year-old age contraception. Abdominal sonography showed abnormal enlargement uterus, ovariohysterectomy performed. Main gross findings uterus were enlarged lesions in two areas the left horn, which had thickened wall yellowish sticky material lumen. Histopathologically, cystic endometrial hyperplasia (CEH)...

Anticoccidial effects of Galla rhois (GR) extract were evaluated in chickens after oral infection with Eimeria tenella. This study was performed using 3-day-old (n=30). The animals divided into 3 groups as follows: GR 0.5%/infected (n=10), untreated/infected and non-infected control (n=10). fed a standard diet supplemented or without for 1 week before E. tenella (10,000 sporulated oocysts per chicken). on assessed by 2 parameters, number fecal body weight gain, the results polymerase chain...

In this study, a medicinal herbal plant, Sanguisorba officinalis, was examined and screened for anti-Helicobacter pylori (H. pylori) activity. Seventy percent ethanol used extraction. For anti-H. activity screening, inhibitory zone tests as an in vitro assay vivo study using Mongolian gerbil (Meriones unguiculatus) model were performed. Also, the safety of compounds evaluated by animal study. As result test, officinalis extract demonstrated strong activities. results studies, therapeutic...

Feline endometrial adenocarcinomas are uncommon malignant neoplasms that have been poorly characterized to date. In this study, we describe a uterine adenocarcinoma in Persian cat with feline leukemia virus infection. At the time of presentation, cat, female chinchilla, was 2 years old. The underwent surgical ovariohystectomy. A cross-section wall revealed thickened horn. tested positive for as detected by polymerase chain reaction. Histopathological examination had metastasized omentum,...