A5044065739- Legionella and Acanthamoeba research
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Water Treatment and Disinfection
- Antifungal resistance and susceptibility
- Travel-related health issues
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Indoor Air Quality and Microbial Exposure
- Vector-Borne Animal Diseases
- Yersinia bacterium, plague, ectoparasites research
- Parasitic Infections and Diagnostics
- Toxoplasma gondii Research Studies
- Pneumonia and Respiratory Infections
- Antibiotic Resistance in Bacteria
- Vector-borne infectious diseases
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Ocular Infections and Treatments
- Mycobacterium research and diagnosis
- Bacterial Infections and Vaccines
- Zoonotic diseases and public health
- Biosensors and Analytical Detection
- Bacillus and Francisella bacterial research
- Infectious Diseases and Mycology
- Advanced Nanomaterials in Catalysis
Instituto de Salud Carlos III
2001-2021
Centro Nacional de Microbiologia
1997-2021
Walter Reed Army Institute of Research
2006
Agence de la Biomédecine
2006
Centre Hospitalier Universitaire de La Réunion
2006
Escuela Nacional de Sanidad
2006
Ajuntament de Barcelona
2001-2002
Institut für Medizinische Informatik, Biometrie und Epidemiologie
1997
TU Dresden
1997
An explosive outbreak of Legionnaires' disease occurred in Murcia, Spain, July 2001. More than 800 suspected cases were reported; 449 these confirmed, which made this the world's largest reported to date. Dates onset for confirmed ranged from June 26 19, with a case-fatality rate 1%. The epidemic curve and geographic pattern 600 competed epidemiologic questionnaires indicated an outdoor point-source exposure northern part city. A case-control study matching 85 patients living outside city...
Summary Amoebae are the natural hosts for Legionella pneumophila and play essential roles in bacterial ecology infectivity to humans. When L. colonizes an aquatic installation, it can persist years despite repeated treatments with disinfectants. We hypothesized that freshwater amoebae important role resistance disinfectants, subsequent resuscitation of viable non‐culturable (VNC) results re‐emergence disease‐causing strain disinfected water source. Our work showed absence Acanthamoeba...
Legionella pneumophila accounts for the majority of cases Legionnaires' disease. By using rabbit antisera, species has been divided into 14 numbered and 1 unnumbered serogroups. To recognize antigenic diversity lipopolysaccharide (LPS) responsible this classification, Dresden LPS MAb panel, containing 98 monoclonal antibodies (MAbs), was created. Each serogroup reference strain possesses at least one specific epitope not found on any other therefore designated serogroup-specific epitope....
Despite more than 50 years of vaccination, pertussis is still an endemic disease, with regular epidemic outbreaks. With the exception Poland, European countries have replaced whole-cell vaccines (WCVs) by acellular (ACVs) in 1990s. Worldwide, antigenic divergence vaccine antigens has been found between strains and circulating strains. In this work, 466 Bordetella isolates collected period 1998–2012 from 13 were characterised multi-locus antigen sequence typing (MAST) toxin promoter (ptxP)...
Abstract Background The repeats in toxin (Rtx) are an important pathogenicity factor involved host cells invasion of Legionella pneumophila and other pathogenic bacteria. Its role escaping the immune system cytotoxic activity is well known. repeated motives modularity make Rtx a multifunctional pathogenicity. Results comparative analysis rtx gene among 6 strains L. showed their structures. Among compared genomes, N-terminal region protein presents highly dissimilar with functionally similar...
Clustered regularly interspaced short palindromic repeats with CRISPR-associated gene (CRISPR-Cas) systems are widely recognized as critical genome defense that protect microbes from external threats such bacteriophage infection. Several isolates of the intracellular pathogen Legionella pneumophila possess multiple CRISPR-Cas (type I-C, type I-F and II-B), yet targets these remain unknown. With recent observation at least one (II-B) plays a non-canonical role in supporting replication,...
Abstract Legionella pneumophila, a Gram-negative bacillus, is the causative agent of Legionnaire’s disease, form severe community-acquired pneumonia. Infection can have high morbidity, with proportion patients requiring ICU admission, and up to 10% mortality, which exacerbated by lack efficacy typical empirical antibiotic therapy against spp. The fastidious nature entire Legionellaceae family historically required inclusion activated charcoal in solid medium remove growth inhibitors,...
The detection in April 1997 of a case nosocomial legionellosis our hospital led to the discovery that both hot- and cold-water circuits were heavily colonized with Legionella pneumophila. Conventional methods for eradication organisms unsuccessful, so copper-silver (Cu-Ag) ionization system continuous chlorination installed. Five months later, number sites decreased from an initial 58.3% 16.7%.
SUMMARY An outbreak of Legionnaire's disease was detected in Pamplona, Spain, on 1 June 2006. Patients with pneumonia were tested to detect Legionella pneumophila antigen urine (Binax Now; Binax Inc., Scarborough, ME, USA), and all 146 confirmed cases interviewed. The related district 2 (22 012 inhabitants), where 45% the lived 50% had visited; 5% neighbouring districts. highest incidence found resident population (3/1000 section (14/1000). All 31 cooling towers analysed. L. Now) four...
To study the persistence of Legionella over time in different disinfected facilities and analysing whether failures bacterial eradication could be result a decrease susceptibility persistent strains to subsequent treatments.A long-term environmental surveillance was carried out three associated with cases Legionnaires' disease (a hospital, fishing boat hotel). Despite several hyperchlorination episodes, amplified fragment length polymorphism, pulsed field gel electrophoresis-SfiI arbitrarily...
ABSTRACT The MICs at which 90% of isolates are inhibited for gemifloxacin, trovafloxacin, and grepafloxacin were low (≤0.01 μg/ml) 271 Legionella when they determined by the broth microdilution method but increased (≥6 dilutions) agar dilution method. This was due to charcoal in medium, as shown progressive decrease concentrations decreased. As free drug is active fraction, binding should be considered.
Aims: To validate identification methods for Legionella pneumophila strains that cannot be serotyped into the known serogroups and to characterize their antigenic diversity. Methods Results: Fifty L. could not serogrouped, but which had been confirmed as by mip gene sequencing, were further identified phenotypically. We used (i) MONOFLUO anti‐Legionella Staining Reagent (Bio‐Rad) (50/50), (ii) an in‐house prepared immunoblot assay detection of pneumophila‐ specific Mip protein epitope (iii)...
It was reported that two mechanics working on a cargo ship under repair in the port of Barcelona had died after having fever. An investigation made into possibility any additional cases and presence Legionella pneumophila they were repairing their hotel. The contaminated water system treated with sodium hypochlorite. Both patients been repeatedly diagnosed as influenza. occurred among those who pump ship's system, while no observed other workers (p = 0.02). Various serogroups L. isolated...
<i>Background:</i><i>Legionella </i>persists in man-made aquatic installations despite preventive treatments. More information about disinfectants could improve the effectiveness of This study tests susceptibility <i>Legionella pneumophila</i> serogroup (sg) 1 against 8 used cooling tower <i>Methods:</i> We determined minimal inhibitory concentration (MIC), bactericidal (MBC) and effect sodium hypochlorite (A), hydrogen peroxide with silver...
Legionella isolates recovered in 21 different Spanish provinces over 8 years from both clinical (67 isolates) and environmental (181) samples, mostly case-associated buildings, are described; 92.5% of were L. pneumophila serogroup 1 (SG1), only five belonging to other species or serogroups: two SG6, SG8 one bozemanii SG1 not clearly related with infection. accounted for 53.6% the environment, followed by (27.6%), SG3 (9.4%) SG6 (7.2%). Three labelled as SG8/10. Subtyping standardized panel...