A5101398749- Virus-based gene therapy research
- Chronic Myeloid Leukemia Treatments
- Pediatric Urology and Nephrology Studies
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Click Chemistry and Applications
- Multiple Myeloma Research and Treatments
- Advanced Fluorescence Microscopy Techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Blood Pressure and Hypertension Studies
- Colorectal Cancer Surgical Treatments
- Gastric Cancer Management and Outcomes
- Cardiac and Coronary Surgery Techniques
- Pelvic floor disorders treatments
- Kidney Stones and Urolithiasis Treatments
- Berberine and alkaloids research
- Chinese history and philosophy
- Urological Disorders and Treatments
- Cardiac Valve Diseases and Treatments
- Chronic Lymphocytic Leukemia Research
- Intraperitoneal and Appendiceal Malignancies
- Organ Transplantation Techniques and Outcomes
- Biomarkers in Disease Mechanisms
- Ureteral procedures and complications
- Diabetic Foot Ulcer Assessment and Management
China Medical University
2008-2024
Clinical Trials New Zealand
2024
Tauranga Hospital
2024
Suzhou Municipal Hospital
2024
First Hospital of China Medical University
2015-2024
Government Communications Headquarters
2024
Jinan University
2003-2023
Peking University
2016-2023
Beijing National Laboratory for Molecular Sciences
2016-2022
Southwest Jiaotong University
2019-2021
Selective manipulation of protein kinases under living conditions is highly desirable yet extremely challenging, particularly in a gain-of-function fashion. Here we employ our recently developed bioorthogonal cleavage reaction as general strategy for intracellular activation individual kinases. Site-specific incorporation trans-cyclooctene-caged lysine place the conserved catalytic lysine, conjunction with partner dimethyl-tetrazine, allowed efficient decaging kinase activity chemically...
Pyroptosis is an inflammatory cell death form triggered by protease-mediated truncation and release of the N-terminal pore-forming domain gasdermin (GSDM) family proteins in various types. We report a Bioorthogonally ACtivatable Base editor (BaseBAC) for situ on-demand initiation cell-type-specific pyroptosis. first made enzymatic activity cytosine base (CBE) switchable establishing bioorthogonal blockage on PAM-interacting residue to control its DNA-binding ability. The resulting BaseBAC...
The approved intravenous adeno-associated virus (AAV) therapies are limited by the widespread prevalence of pre-existing anti-AAV antibodies in general population, which known to restrict patients' ability receive gene therapy and limit transfection efficacy vivo. To address this challenge, we have developed a novel recombinant human immunoglobulin G degrading enzyme KJ103, characterized low immunogenicity clinical value for elimination transfer. Herein, conducted two randomized, blinded,...
We report the genetically encoded chemical decaging strategy for protein activation in living bacterial cells. In contrast to metabolically labile photocaging groups inside Escherichia coli, our that relies on inverse electron-demand Diels-Alder (iDA) reaction is compatible with intracellular environment of bacteria, which can be a general tool gain-of-function study given prokaryotic systems. By applying this situ indole-producing enzyme TnaA, we built an orthogonal and chemically inducible...
Berberine downregulated miR-19a/92a cluster expression in multiple myeloma (MM) cells.The cell viability of MM cells after berberine treatment was measured by CCK8 assay. qRT-PCR assay validated cells. TAM database analyzed miR-19a/92a-associated disease. miREnvironment revealed that effects environmental factors on the cluster. By targeting seed region miRNA, role t-anti-miR-19a/92a evaluated proliferation, migration, and colony formation.Berberine inhibited miR-19a/92a. Seven kinds...
Achieving a complete cure while maintaining continence constitutes considerable challenge in the treatment of patients with high anal fistula. This study aimed to evaluate effectiveness loose combined cutting seton (LCCS) for treating intersphincteric fistula.Consecutive fistula who underwent LCCS were retrospectively enrolled. Patient data including demographics, medical history, comorbidities, details fistula, operative procedure, and prognosis collected. Postoperative pain was assessed...
Civic organizations have long been heralded for promoting voluntarism and civic participation, yet observational studies, facing endogeneity concerns, struggled to solidify the causal underpinnings of neo-Tocquevillian theory. Furthermore, empirical examination their recruitment efficacy relative state entities, particularly in crises or authoritarian settings, remains scarce. Our survey experiment COVID-period China provides a twofold crucial case study. By embodying both “most likely case”...
Background: Despite being a major focus of medical research for decades, rectal cancer remains threat to human health. This study aimed compare and analyze the diagnostic value transrectal ultrasonography (TRUS) adenoma early-stage before after gastrointestinal agent instillation.
The accuracy of diagnosing tumors may be improved significantly by detecting the microvascular distribution. Indeed, contrast-enhanced ultrasonography (CEUS) has shown a distinct advantage in microvasculature. This study aimed to determine angiogenic characteristics VX2 rabbits using CEUS. A total 17 were injected with 0.5 ml cell suspension into muscles both hind legs prepare tumor models. At 14, 21, 28 and 35 days after inoculation, CEUS was performed on 0.3 SonoVue following local...
This study explores the prevalence of subthreshold depression (SubD) and its association with factors in type 2 diabetes mellitus (T2DM) patients. cross-sectional involved 808 outpatients T2DM from ten hospitals Beijing between September 2015 January 2016. All participants completed Patient Health Questionnaire 9-item (PHQ-9) to evaluate depressive status, scores 5 14 considered SubD. Conditional logistic regression was conducted investigate variables associated SubD patients comprised 11.6%...
Objective . This study aims to explore the relationship between autonomic nerve dysfunction—assessed by cardiovascular neuropathy risk score (CAN-RS)—and ocular abnormality in Chinese type 2 diabetes mellitus (T2DM). Method is a cross-sectional study. A total of 335 subjects with T2DM were enrolled. The state visual acuity, lens, vitreous, and fundus tested professional ophthalmic instruments. electrochemical skin conductance (ESC) hands feet was measured SUDOSCAN, from which (CAN-RS)...
Persistent proteinuria is an important factor contributing to the progression of diabetic nephropathy. The present randomized double-blind placebo-controlled multicenter clinical study evaluated efficacy and safety telmisartan combined with antioxidant probucol in reducing urinary protein levels patients type 2 diabetes (T2D).Patients T2D 24-h 0.5-3 g were enrolled randomly assigned one two groups: a or + group. Both groups given 80 mg q.d. for 48 weeks. group was 500 b.i.d. first 24 weeks,...
Resistance to the BCR-ABL inhibitor imatinib mesylate poses a major problem for treatment of chronic myeloid leukemia. Imatinib resistance often results from secondary mutation in that interferes with drug binding. However, sometimes there is no BCR-ABL, and basis such BCR-ABL-independent remains be elucidated. SOS1, guanine nucleotide exchange factor Ras protein, affects sensitivity imatinib. The depletion SOS1 markedly inhibits cell growth either vitro or vivo significantly increases...
In patients with unclear ascites, a thickened greater omentum variable nodules is usually encountered high-frequency insonation. The purpose of our study was to assess the importance in indicating origin ascites.In suggested for biopsy omentum, if were found sonograms recorded, and during biopsy, 2 passes made each procedure nodule thickest region without nodules, respectively. A considered successful specific benign or malignant diagnosis made.In 258 undergoing sonographically guided...