A5045094516- Neonatal Health and Biochemistry
- CRISPR and Genetic Engineering
- Birth, Development, and Health
- Nutrition, Genetics, and Disease
- Epigenetics and DNA Methylation
- Genetics, Aging, and Longevity in Model Organisms
- Pancreatic function and diabetes
- Plant tissue culture and regeneration
- DNA Repair Mechanisms
- Genetic diversity and population structure
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Cancer-related gene regulation
- Nutritional Studies and Diet
- Corneal Surgery and Treatments
- 3D Printing in Biomedical Research
- Chromosomal and Genetic Variations
- Pluripotent Stem Cells Research
- Glycosylation and Glycoproteins Research
- Gestational Diabetes Research and Management
- Health, Environment, Cognitive Aging
- Neurofibromatosis and Schwannoma Cases
- Folate and B Vitamins Research
- Animal Diversity and Health Studies
- Genetic Associations and Epidemiology
Institute for Anthropological Research
2018-2025
International Centre for Genetic Engineering and Biotechnology
2013-2023
University of Zagreb
2013-2016
AREA Science Park
2013
Epigenetic studies relied so far on correlations between epigenetic marks and gene expression pattern. Technologies developed for epigenome editing now enable direct study of functional relevance precise modifications regulation. The reversible nature modifications, including DNA methylation, has been already exploited in cancer therapy remodeling the aberrant landscape. However, this was achieved non-selectively using inhibitors. at specific loci represents a novel approach that might...
Null mutations in the UGT1A1 gene result Crigler–Najjar syndrome type I (CNSI), characterized by severe hyperbilirubinemia and constant risk of developing neurological damage. Phototherapy treatment lowers plasma bilirubin levels, but its efficacy is limited liver transplantation required. To find alternative therapies, we applied AAV liver-specific therapy to a lethal mouse model CNSI. We demonstrated that single neonatal hUGT1A1 transfer was successful therapeutic effect lasted up 17...
Palbociclib, ribociclib and abemaciclib were recently approved as chemotherapeutic agents are currently in the post-marketing surveillance phase. They used combination with aromatase inhibitors anastrozole letrozole or antiestrogen fulvestrant for HR+, HER2- breast cancer treatment. Here, a novel bioanalytical LC-ESI-MS/MS method was developed quantitation of these six drugs human plasma. The samples prepared by simple protein precipitation followed solvent evaporation. A Kinetex biphenyl...
Limbal stem cells (LSCs) are of paramount importance in corneal epithelial tissue repair. The cornea becomes opaque case limbal cell deficiency (LSCD), which may cause serious damage to the ocular visual function. There many techniques restore damaged epithelium, one is transplantation healthy cultured LSCs, usually onto a human amniotic membrane or bio-based engineered scaffolds recent years. In this study, melt electrospun polylactic acid (PLA) was modified by silk fibroin gelatin and...
Anemone sensu lato (including Pulsatilla and Hepatica), tribe Anemoneae (Ranunculaceae), is arranged into two subgenera, Anemonidium, with basic chromosome numbers x = 8 7, respectively. We elucidated the level of divergence 5S rDNA unit arrays between determined intra-individual interspecific sequence variation tested phylogenetic signal in revealing origin polyploid species. High nucleotide diversity presence array length variants pseudogenes indicate that weak homogenization forces have...
Moderate neonatal jaundice is the most common clinical condition during newborn life. However, a combination of factors may result in acute hyperbilirubinemia, placing infants at risk developing bilirubin encephalopathy and death by kernicterus. While are known, mechanisms acting to reduce susceptibility neurotoxicity remain unclear. The presence modifier genes modulating bilirubin-induced brain damage increasingly being recognised. Abcb1 Abcc1 members ABC family transporters have been...
Cas3 nuclease-helicase is part of CRISPR immunity systems in many bacteria and archaea. In type I CRISPR, nuclease degrades invader DNA that has been base-paired to crRNA as an R-loop within a "Cascade" complex. An DNA-RNA hybrid includes displaced single-strand loop. Purified from E. coli the archaeon M. thermautrophicus can process R-loops without DNA/RNA sequence specificity Cascade. This potential affect other aspects microbial biology involve R-loops. Regulatory RNAs host cell proteins...
Among ruthenium complexes studied as anticancer metallodrugs, NKP-1339, NAMI-A, RM175, and RAPTA-C have already entered clinical trials due to their potent antitumor activity demonstrated in preclinical studies reduced toxicity comparison with platinum drugs. Considering the advantages of ruthenium-based drugs cytostatic organometallic triazole- coumarin-derived ligands, we set out synthesize Ru(II) coumarin-1,2,3,-triazole hybrids (L) general formula [Ru(L)(p-cymene)(Cl)]ClO
The Crigler-Najjar Syndrome Type I (CNSI) is a rare genetic disorder caused by mutations in the Ugt1a1 gene. It characterized unconjugated hyperbilirubinemia that may result severe neurologic damage and death if untreated. To date, liver transplantation only curative treatment. With aim of generating mutant cell lines Ugt1 gene, we utilized TALEN technology to introduce site-specific exon 4. We report fast efficient method perform gene knockout tissue culture cells, based on use pairs...
Longevity is a hallmark of successful ageing and complex trait with significant genetic component. In this study, 43 single nucleotide polymorphisms (SNPs) were chosen from the literature genotyped in Croatian oldest-old sample (85+ years, size (N) = 314), order to determine whether any these SNPs have effect on reaching age thresholds for longevity (90+ N 212) extreme (95+ 84). The best models selected both survival ages using multivariate logistic regression. model 90, nine explained 20%...
(AAV)-mediated episomal gene replacement therapy for monogenic liver disorders is currently limited in pediatric settings due to the loss of vector DNA, associated with hepatocyte duplication during growth. Genome editing a promising strategy leading permanent and specific genome modification that transmitted daughter cells upon proliferation. Using targeting, we previously rescued neonatal lethality mice Crigler-Najjar syndrome. This rare disease characterized by severe unconjugated...
Genome-wide association studies and candidate gene have identified several genetic variants that might play a role in achieving longevity. This study investigates interactions between pairs of those single nucleotide polymorphisms (SNPs) their effect on survival above the age 85 sample 327 Croatian individuals. Although none SNPs individually showed significant this sample, 14 359 tested (between not LD) reached level nominal significance (p<0.05), showing potential late-life survival....
Despite the advancements in targeted therapy for BRAFV600E-mutated metastatic colorectal cancer (mCRC), development of resistance to BRAFV600E inhibition limits response rate and durability treatment. Better understanding mechanisms BRAF inhibitors will facilitate design novel pharmacological strategies BRAF-mutated mCRC. The aim this study was identify protein candidates involved acquired inhibitor vemurafenib colon cells using an integrated proteomics approach. Bioinformatic analysis...
High pre-pregnancy body mass index (BMI) and excessive gestational weight gain (GWG) are significant risk factors for maternal neonatal health.To assess BMI GWG during pregnancy their association with different characteristics in the transitional Mediterranean population from Eastern Adriatic islands.Two hundred sixty-two mother-child dyads CRoatian Islands' Birth Cohort Study (CRIBS) were included study. Chi-square test, ANOVA, regression analysis used to test between selected...
Genome editing tools, such as TALEN (transcription activator-like effector nuclease) or CRISPR-Cas9 (CRISPR-associated protein-9 systems, enable functional studies by targeted gene knockout.They introduce double-stranded breaks (DSBs) into a DNA molecule in sequence-specific manner, thereby stimulating the error-prone non-homologous end joining repair mechanism, leading to probable inactivation when coding sequence is targeted.Vectors for expression of and Cas9-based constructs targeting...
The Crigler-Najjar Syndrome Type I (CNSI) is a rare monogenic disease caused by null mutations in the UGT1A1 gene, resulting bilirubin conjugation deficiency and unconjugated hyperbilirubinemia, with lifelong risk of neurological damage, kernicterus death. Current clinical practice consists phototherapy treatment (PT) for more than 10-12 h/day, profoundly affecting (social) life quality. However, PT becomes less effective age, leaving liver transplantation as only therapeutic option, which...