In the context of stroke and revascularization therapy, brain ischemia-reperfusion injury is a significant challenge that leads to oxidative stress inflammation. Central cell's intrinsic immunity cGAS-STING pathway, which typically activated by unusual DNA structures. The involvement oxidized mitochondrial (ox-mtDNA)-an byproduct-in this type neurological damage has not been fully explored. This study among first examine effect ox-mtDNA on innate neurons following injury. Using rat model...

Resveratrol possesses beneficial biological effects, which include anti-oxidant, anti-inflammatory and anti-carcinogenic properties. Recently, resveratrol has been shown to exhibit neuroprotective effects in models of Parkinson's disease, cerebral ischemia Alzheimer's disease. However, its on vascular dementia remain unclear. The present study established a rat model using permanent bilateral common carotid artery occlusion. At 8-12 weeks after induction, rats were intragastrically...

Widespread death of transplanted mesenchymal stem cells (MSCs) hampers the development cell therapy for Alzheimer disease (AD). Cell pre-conditioning might help cope with this challenge. We tested whether let-7f-5p-modified MSCs could prolong survival after transplantation. When exposed to Aβ25-35 in vitro, showed significant early apoptosis decrease let-7f-5p levels and increased caspase-3 expression. Upregulating microRNA alleviated Aβ25-35-induced by decreasing levels. After computerized...

MicroRNAs are members of the family noncoding small RNAs that regulate gene expression either by inhibiting mRNA translation or promoting degradation at posttranscriptional level. They play an important role in differentiation bone marrow-derived mesenchymal stem cells (MSCs) into neurons. However, microRNAs this process remains to be poorly understood. Studies have shown Notch signaling is involved regulating MSC differentiation. Here, we found microRNA-9 could promote neuronal Using...

Resveratrol is a natural polyphenol widely present in plants, particularly the skin of red grapes and wine. It possesses wide range biological effects exhibits neuroprotective numerous diseases. However, data evaluating resveratrol vascular dementia (VaD) are lacking. In study, permanent, bilateral common carotid artery occlusion rat model was used to study on VaD. The Morris water maze test spatial learning memory performance rats. expression levels Bax, Bcl‑2, cleaved caspase‑3...

In this study, the putative involvement of Notch-1 signaling pathway in neuronal differentiation bone marrow mesenchymal stem cells (BMSCs) was investigated through RNA interference (RNAi). We found that mNotch-1shRNA could efficiently block expression BMSCs. After induction, immunohistochemistry, reverse transcriptase-PCR and Western blot analyses, all indicated neuron-specific markers such as enolase neurofilament 200 much higher BMSCs than control groups, whereas glial fibrillary acidic...

Autophagy is involved in cell differentiation. We present evidence that autophagy activated during β-mercaptoethanol (β-ME)-induced neuronal differentiation of bone marrow mesenchymal stem cells (MSCs), which mammalian target rapamycin (mTOR) signaling important. mTOR activity declined after being transported from the nucleus to cytoplasm. Using 3-methyladenine (3-MA) and regulate mTOR, it was found efficiency affected.

Abstract Mesenchymal stem cells (MSCs) transplantation has emerged as a potential therapeutic approach for Alzheimer's disease (AD). However, the poor proliferation capacity and low survival rate of engrafted MSCs in hostile microenvironment AD limit their efficiency. Lin28B is conserved RNA‐binding protein associated with cell self‐renewal survival. The purpose present study was to explore whether lin28B might influence functions implanted strengthen neuroprotective AD. A gain‐of‐function...

Anti-IgLON family member 5 (IgLON5) disease is a rare autoimmune encephalitis, characterized by sleep problems, cognitive decline, gait abnormalities, and bulbar dysfunction. Anti-leucine-rich glioma-inactivated 1 (LGI1) encephalitis dysfunction, mental disorders, faciobrachial dystonic seizures (FBDS), hyponatremia. Various studies report that coronavirus 2019 (COVID-19) have an effect on the nervous system induce wide range of neurological symptoms. Autoimmune one complications in severe...

Parkinson's disease (PD) is the second most common neurodegenerative in humans. The hormone α-melanocyte-stimulating (α-MSH) has been reported to be neuroprotective previous studies. aim of this study investigate effects α-MSH against neurotoxicity 1-methyl-4-phenylpyridinium (MPP+). Our results indicated that treatment with M17 cells attenuated MPP+-induced oxidative stress, embodied by exacerbated reactive oxygen species and protein carbonyls. In addition, we found could improve...

Inflammation is a key element in the pathophysiology of ischemic stroke. The current effective treatments for stroke are almost exclusively based on reperfusion occluded vessels. An increasing number studies attempting to develop better treatment strategies by investigating involvement neuroinflammation pathogenesis. There growing focus inflammatory response glial cells within neurovascular unit (NVU) stroke, while pericytes, despite their central position NVU and having interactions with...

Objective . Even though there is a therapeutic potential to treat Alzheimer’s disease (AD) with neural cell replenishment and replacement, immunological rejections of stem transplantation remain challenging risk. Autologous cells from AD patients however may prove be promising candidate. Therefore, we studied the neuronal differentiation efficiency bone marrow mesenchymal (MSCs) APP695 transgenic mice, which share features human AD. Method Cultured MSCs mice are used; was assessed by...

We aimed to explore the difference in coagulation function between healthy individuals and patients with anti-N-methyl-D-aspartate receptor (anti-NMDAR) encephalitis its relationship disease severity.We retrospectively compared 161 first-attack anti-NMDAR 178 individuals. The association D-dimer levels severity was analyzed using binary logistic regression. Receiver operating characteristic (ROC) curves were used analyze predictive value of for encephalitis.Compared control individuals, had...

Objective To investigate changes in the expression of prepro-orexin and orexin receptor-1 ( OX1R) following permanent middle cerebral artery occlusion MCAO ) with or without preconditioning through electrical stimulation cerebellar fastigial nucleus (FNS). Methods Wistar rats were subjected to randomly divided into 5 groups: a sham-operated control group (PO), an FNS + shamoperated (FNS-PO) , ischemia group, (FNS-PI) injury (FNL-FNS-PI). Each was subgroups according time at which animals...

Objective To investigate the role of caveolin-1 in bone marrow mesenchymal stem cells(MSCs) differentiating into neuron-like cells by transfecting MSCs with small interfering RNA(siRNA). Methods Construct siRNA and then transfect cultured vitro. Cell growth was observed an inverted phase contrast microscope. The survival ratio determined MTT before transfection,24 hour, 3 days after transfection,respectively. induced to differentiate by-ME. neural cell specific marker Nestin,NSE,GFAP were...