Pediatric acute myeloid leukemia (AML) is a heterogeneous disease and remains clinically challenging. Currently chemotherapies are frequently associated with treatment-related death long-term side effects. Therefore, alternative approaches lower toxicity highly desired. Ginsenosides metabolites the main ingredients responsible for multiple pharmaceutical functions of ginseng, which one most commonly consumed herbal medicines world widely. In present study, we demonstrated that compound K,...

Growing evidence suggests that circular RNAs (circRNAs) play a significant role in regulating cancer initiation and metastasis. Osteosarcoma (OS) is sophisticated disease with various genes activated or silenced. In this study, we defined novel cancer-related circRNA, circ_0000502 OS progression. qRT-PCR was conducted to detect its expression level tissue samples cell lines. addition, the clinical significance of investigated. Afterwards, gain-of-function loss-of-function vitro assays were...

Abstract Proteolysis‐targeting chimeras (PROTACs) are essential bifunctional molecules that target proteins of interest (POIs) for degradation by cellular ubiquitination machinery. Despite significant progress made in understanding PROTACs' functions, their therapeutic potential remains largely untapped. As a result the success highly flexible, scalable, and low‐cost mRNA therapies, as well advantages first generation peptide PROTACs (p‐PROTACs), we present time an engineering (m‐PROTACs)...

MicroRNAs (miRNAs) have been widely reported to important regulatory roles in various human tumors, including osteosarcoma (OS). The aim of this study was focus on the role less well-known miRNA-567 (miR-567) OS. We found expression miR-567 significantly reduced OS tissues and cell lines (MG-63, U2OS Saos-2) compared with adjacent normal osteoblastic cells (hFOB), respectively. Moreover, exogenous overexpression inhibited proliferation, migration invasion by CCK-8, Transwell assays, further...

Abstract Distinct metabolic vulnerabilities of cancer cells compared with normal can potentially be exploited for therapeutic targeting. Deficiency argininosuccinate synthetase-1 (ASS1) in pancreatic cancers creates auxotrophy the semiessential amino acid arginine. We explored potential depleting exogenous arginine via pegylated deiminase (ADI-PEG20) treatment as an adjunct to radiotherapy. evaluated efficacy human cell lines and xenografts ADI-PEG20 radiation clonogenic assays tumor growth...

Background: Metastasis is the primary cause of death in colorectal cancer (CRC); underlying mechanisms remain partly unknown. In this study, we aim to investigate value HOXA11-AS survival evaluation and potential role HOXA11-AS/miR-149-3p axis CRC metastasis. Methods: The expressions HOXA11-AS, both obtained samples adjacent noncancerous tissues, were analyzed evaluation. Competing endogenous RNAs (CeRNAs) Analysis employed reveal relationship between miR-149-3p. It was further confirmed by...

To explore the effect of trichostatin A (TSA) on apoptosis and acetylated histone H3 levels in gastric cancer cell lines BGC-823 SGC-7901.The TSA growth inhibition was examined by MTT, fluorescence microscopy PI single-labeled flow cytometry. The level detected Western blot.TSA induced SGC-7901 a dose time-dependent manner. Apoptotic cells varied significantly between treated groups (37.5 ng/mL 72 h for line 75 line) control group (0.85+/-0.14 vs 1.14+/-0.07, P=0.02; 0.94+/-0.07 1.15+/-0.06,...

Esophageal squamous cell carcinoma (ESCC) is one of the most common cancers in China, accompanied by an extremely high mortality rate. Chlorogenic acid (CGA) a small-molecule compound, that has been shown to have wide range biological activities, including antitumor. However, efficacy and molecular mechanism CGA on ESCC remains unknown. In this study, we confirmed inhibition proliferation cells, as well reduction xenograft volume vivo. addition, also suppressed both migration invasion cells...

Aims and background Arsenic trioxide (ATO), an antitumor agent, is widely used for treating acute promyelocytic leukemia (APL), in which it induces apoptosis. In most solid tumors, ATO disturbs the cell cycle instead of inducing We aimed to determine exact mechanism underlying different response APL compared with tumors. Methods A proteomics-based screening was identify ATO-associated proteins human esophageal squamous carcinoma line, Eca109. The expression levels Annexin A1 (ANXA1) 4 types...

MicroRNA (miR)-106b-5p has been reported to act as both an oncogene and tumor suppressor in several tumors. The aim of the present study was investigate biological function miR-106b-5p osteosarcoma (OS). expression observed be significantly increased OS tissues cell lines. MTT assay flow cytometry analysis determined that inhibitor transfection suppressed proliferation induced cycle G0/G1 phase arrest. Furthermore, bioinformatics a luciferase reporter demonstrated cyclin-dependent kinase 1A...

Osteosarcoma (OS) is a common primary malignant bone tumor. This study aimed to explore the biological role of long on-coding RNA (lncRNA) CASC9 and its regulatory mechanism in OC.The expressions OS cells tissues were measured using qRT-PCR. The functional OC was studied MTT assay, colony formation transwell invasion xenograft tumor assay. In addition, function determined luciferase reporter Western blot used analyze protein our paper.LncRNA found be up-regulated OS. Knockdown inhibited...

TGFB induced factor homeobox 1 (TGIF1), a transcriptional corepressor, has been reported to be involved in tumorigenesis and cancer development. However, the role of TGIF1 growth metastasis esophageal is poorly studied. In present study, it was found that highly expressed tissues cell lines. The silencing by siRNA interference significantly inhibited proliferation, migration, invasion epithelial‑mesenchymal transition (EMT) process KYSE‑150 cells, promoted apoptosis. Correspondingly,...

Background. Hypoxia exerts pressure on cells and organisms, this can occur under both pathological nonpathological conditions. There are many reports confirmed that hydroxytyrosol has good in vitro antioxidant activity, while the research about hypoxia-mediated cell damage is still unclear. Purpose. The aim of study was to investigate effect mechanism damage. Methods. We studied effects content reactive oxygen species, change enzymes activity SOD, CAT, GSH-Px oxidation products MDA GSH,...

MicroRNAs (miRs) have been demonstrated to be involved in the development and progression of osteosarcoma (OS), but molecular mechanism still remains fully investigated. The present study investigated function miR-148a OS, as well its underlying mechanism. Our data showed that was significantly downregulated OS tissues compared their matched adjacent normal tissues, also cell lines human osteoblast cells. Low expression associated with tumor a poor prognosis for patients. Rho-associated...

Circular RNA (circRNAs) Fibronectin Type III Domain Containing 3B (FNDC3B) (circFNDC3B) has been revealed to be involved in the progression of oesophageal squamous cell carcinoma (ESCC). Hence, potential regulatory network circFNDC3B ESCC was further investigated. Levels genes and proteins were examined by qRT-PCR Western blot. In vitro assays performed using colony formation assay, 5-Ethynyl-2'-deoxyuridine (EdU) flow cytometry, wound healing transwell assay. The target relationship between...

Colon cancer (COAD) is a prevalent gastrointestinal tumor, composed of few stem cells (CSCs). High expression RNF183 drives colorectal metastasis, but its role in COAD cell stemness still unclear. Bioinformatics analyzed and enriched pathway tissue. IHC protein tumor CD133 + CD44+ CSCs were sorted by flow cytometry, or was detected qPCR, western blot immunofluorescence. CCK-8 assay assessed viability, sphere formation tested sphere-forming ability. Western measured markers. qPCR assayed...

This study was aimed to explore the effect of VX-680, an aurora inhibitor, on proliferation and apoptosis K562, KCL22 cell lines CD34⁺ cells from chronic myeloid leukemia (CML) patients in vitro. The K562 detected by CCK-8 method. Apoptosis Annexin V-PI labeling flow cytometry. colony forming ability bone marrow derived CML donors determined test. results showed that treatment with VX-680 20-100 nmol/L for 3 days could obviously inhibit a concentration-dependent manner (P < 0.01)....

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&lt;div&gt;Abstract&lt;p&gt;Distinct metabolic vulnerabilities of cancer cells compared with normal can potentially be exploited for therapeutic targeting. Deficiency argininosuccinate synthetase-1 (ASS1) in pancreatic cancers creates auxotrophy the semiessential amino acid arginine. We explored potential depleting exogenous arginine via pegylated deiminase (ADI-PEG20) treatment as an adjunct to radiotherapy. evaluated efficacy human cell lines and xenografts ADI-PEG20 radiation clonogenic...