A5100461510- Reproductive Physiology in Livestock
- Ruminant Nutrition and Digestive Physiology
- Liver Disease Diagnosis and Treatment
- Lipid metabolism and disorders
- Genetic and phenotypic traits in livestock
- Lipid metabolism and biosynthesis
- Peroxisome Proliferator-Activated Receptors
- Autophagy in Disease and Therapy
- Cancer-related molecular mechanisms research
- Adipose Tissue and Metabolism
- Diet, Metabolism, and Disease
- Cancer, Hypoxia, and Metabolism
- Metabolism, Diabetes, and Cancer
- Mechanical and Optical Resonators
- Biosimilars and Bioanalytical Methods
- Growth Hormone and Insulin-like Growth Factors
- Cancer Genomics and Diagnostics
- Genomics, phytochemicals, and oxidative stress
- Effects of Environmental Stressors on Livestock
- Advanced MEMS and NEMS Technologies
- Pesticide Residue Analysis and Safety
- Endoplasmic Reticulum Stress and Disease
- Glioma Diagnosis and Treatment
- Kruppel-like factors research
- Metabolism and Genetic Disorders
Capital Medical University
2017-2025
Jilin Medical University
2010-2025
Jilin University
2016-2025
Beijing Children’s Hospital
2019-2025
Shenzhen Second People's Hospital
2021-2024
Hunan University
2024
Yangtze University
2021-2024
Ji Hua Laboratory
2024
Tianjin Medical University
2024
Zhejiang University
2020-2023
The risks of cancer and other degenerative diseases caused by reactive oxygen species electrophiles can be reduced the up-regulation detoxifying enzymes. A major mechanism whereby these protective enzymes are induced occurs through activation antioxidant response element (ARE) oxidative-stress sensor protein Kelch-like ECH-associated 1 (Keap1) transcription factor NF-E2-related 2 (Nrf2). Under basal conditions, Keap1 sequesters Nrf2 in cytoplasm binding to its Neh2 domain. Chemical inducers...
A simple procedure for selecting the correct weighting factors linear and quadratic calibration curves with least-squares regression algorithm in bioanalytical LC-MS/MS assays is reported. The factor determined by relationship between standard deviation of instrument responses (σ) concentrations (x). 1, 1/x, or 1/x2 should be selected if, over entire concentration range, σ a constant, σ2 proportional to x, respectively. For first time, we demonstrated detailed scientific reasoning, solid...
Abstract Somatic mutations in cytosolic or mitochondrial isoforms of isocitrate dehydrogenase (IDH1 IDH2, respectively) contribute to oncogenesis via production the metabolite 2-hydroxyglutarate (2HG). Isoform-selective IDH inhibitors suppress 2HG and induce clinical responses patients with IDH1- IDH2-mutant malignancies. Despite promising activity inhibitors, mechanisms that mediate resistance inhibition are poorly understood. Here, we describe four cases identify mutant isoform switching,...
Abstract Isocitrate dehydrogenase (IDH) 1 and 2 mutations result in overproduction of D-2-hydroxyglutarate (2-HG) impaired cellular differentiation. Ivosidenib, a targeted mutant IDH1 (mIDH1) enzyme inhibitor, can restore normal differentiation results clinical responses subset patients with mIDH1 relapsed/refractory (R/R) acute myeloid leukemia (AML). We explored mechanisms ivosidenib resistance 174 confirmed R/R AML from phase trial. Receptor tyrosine kinase (RTK) pathway were associated...
The female parts of hops (Humulus lupulus L.) show estrogenic effects as well cancer chemopreventive potential. We analyzed the mechanism by studying its antioxidative activities and effect on detoxification a potentially toxic quinone (menadione). enzyme reductase [(NAD(P)H:quinone oxidoreductase, QR] protects against quinone-induced toxicity has been used marker in chemoprevention studies. Although hop extract was only weak quencher free radicals formed from 1,1-diphenyl-2-picrylhydrazyl,...
Under basal conditions, the interaction of cytosolic protein Keap1 (Kelch-like ECH-associated 1) with transcription factor nuclear factor-E(2)-related 2 (Nrf2) results in a low level expression cytoprotective genes whose promoter region contains antioxidant response element (ARE). Alkylation one or more 27 cysteine sulfhydryl groups human is proposed to lead Nrf2 accumulation, upregulation gene by ARE, and prevention degenerative diseases, such as cancer. Therefore, identification most...
Dairy cows with ketosis are characterized by oxidative stress, hepatic damage, and hyperketonemia. Acetoacetate (AA) is the main component of ketone bodies in ketotic cows, associated above pathological process. However, potential mechanism was not illuminated. Therefore, aim this study to investigate AA‐induced damage cows. Compared healthy exhibited severe stress damage. Moreover, extent had a positive relationship AA levels. In vitro, treatment increased reactive oxygen species (ROS)...
β -Hydroxybutyric acid (BHBA) has neuroprotective effects, but the underlying molecular mechanisms are unclear. Microglial activation plays an important role in neurodegenerative diseases by producing several proinflammatory enzymes and cytokines. The current study investigates potential whereby BHBA affects expression of potentially proteins cultured murine microglial BV-2 cells stimulated with lipopolysaccharide (LPS). results showed that significantly reduced LPS-induced protein mRNA...
The transition period is a severe challenge to dairy cows. Glucose supply cannot meet demand and body fat mobilized, potentially leading negative energy balance (NEB), ketosis, or fatty liver. Propionate produces glucose by gluconeogenesis, which depends heavily on the number species of microbes. In present study, we analyzed rumen microbiome composition cows in period, with nonperinatal terminal restriction fragment length polymorphism (TRFLP) analysis 16S rRNA genes quantitative PCR. TRFLP...
Background/Aims: Dairy cows with ketosis are characterized by oxidative stress and hepatic damage. The aim of this study was to investigate the apoptotic status ketotic cows, as well underlying apoptosis pathway. Methods: blood aspartate aminotransferase (AST), alanine (ALT), glutamate dehydrogenase (GLDH) gamma-glutamyl transferase (GGT) activities haptoglobin (HP), serum amyloid A (SAA) cytokeratin 18 neo-epitope M30 (CK18 M30) concentrations were determined commercially available kits...
Background/Aims: ß-hydroxybutyrate (BHBA) is the major component of ketone bodies in ketosis. Dairy cows with ketosis often undergo oxidative stress. BHBA related to inflammation involved other diseases dairy cattle. However, whether can induce inflammatory injury cow hepatocytes and potential mechanism this induction are not clear. The NF-κB pathway plays a vital role response. Methods: Therefore, study evaluated stress, pro-inflammatory factors cultured calf treated different...
Background/Aims: Subacute ruminal acidosis (SARA) is a common disease in high-producing lactating cows. Rumenitis the initial insult of SARA and associated with high concentrations histamine produced rumen dairy cows during SARA. However, exact mechanism remains unclear. The objective current study to investigate whether induces inflammation epithelial cells underlying this process. Methods: Bovine were cultured treated different pyrrolidine dithiocarbamate (PDTC, an NF-κB inhibitor) pH...
Abstract Non‐alcohol fatty liver disease (NAFLD) is a common which causes serious damage. Geniposide (GEN), kind of iridoid glycoside extracted from Gardenia jasminoides fruit , has many biological effects, such as resistance to cell damage and anti‐neurodegenerative disorder. Lipid accumulation was obvious in tyloxapol‐induced oil acid (OA) with palmitic (PA)‐induced HepG2 cells compared the control groups while GEN improved increasing conditions. significantly lessened total cholesterol...
Subacute ruminal acidosis (SARA) is a metabolic disease in high-producing dairy cattle, and accompanied by rumenitis. However, the mechanism of rumenitis remains unclear. Therefore, aim this study was to investigate molecular cows with SARA. The results showed that SARA displayed high concentrations volatile fatty acids, lactic acid lipopolysaccharide (LPS). Furthermore, blood LPS acute phase proteins haptoglobin, serum amyloid-A, binding protein were significantly higher than control cows....
Identifying safe and effective compounds that target to mitophagy eliminate impaired mitochondria may be an attractive therapeutic strategy for non-alcoholic fatty liver disease. Here, we investigated the effects of cyanidin-3-O-glucoside (C3G) on disease (NAFLD) underlying mechanism.Non-alcoholic was induced by a high-fat diet 16 weeks. C3G administered during last 4 In vivo, recombinant adenoviruses AAV8 were used overexpression knockdown PTEN-induced kinase 1 (PINK1), respectively. AML-12...
Abstract Allosteric inhibitors of mutant IDH1 or IDH2 induce terminal differentiation the leukemic blasts and provide durable clinical responses in approximately 40% acute myeloid leukemia (AML) patients with mutations. However, primary resistance acquired to drugs are major issues. To understand molecular underpinnings IDH (IDHi), we perform multipronged genomic analyses (DNA sequencing, RNA sequencing cytosine methylation profiling) longitudinally collected specimens from 60 IDH1-...
Summary Background Mutant isocitrate dehydrogenase 1 and 2 (IDH1/IDH2) enzymes produce the oncometabolite D-2-hydroxyglutarate (2-HG). Ivosidenib (AG-120) is a targeted mutant IDH1 inhibitor under evaluation in phase dose escalation expansion study of IDH1-mutant advanced solid tumors including cholangiocarcinoma, chondrosarcoma, glioma. We explored pharmacokinetic (PK) pharmacodynamic (PD) profiles ivosidenib these populations. Methods was administered orally once (QD) or twice (BID) daily...
Acetaminophen (APAP) overdose is the predominant cause of drug-induced liver injury worldwide. The macroautophagy/autophagy-lysosomal pathway (ALP) involved in APAP hepatotoxicity. TFEB (transcription factor EB) promotes expression genes related to autophagy and lysosomal biogenesis, thus, pharmacological activation TFEB-mediated ALP may be an effective therapeutic approach for treating APAP-induced injury. We aimed reveal effects narirutin (NR), main bioactive constituents isolated from...
Abstract PARP inhibitors and HDAC have been approved for the clinical treatment of malignancies, but acquired resistance or limited effects on solid tumors with a single agent remain as challenges. Bioinformatics analyses combination experiments had demonstrated synergistic in triple-negative breast cancer. A series novel dual were rationally designed synthesized, these molecules exhibited high enzyme inhibition activity excellent antitumor vitro vivo. Mechanistically, induced BRCAness to...
Chromosome dimers, formed by homologous recombination between sister chromosomes, normally require cell division to be resolved into monomers site‐specific at the dif locus of Escherichia coli . We report here that it is not in fact per se required for dimer resolution but action cytoplasmic domain FtsK, which a bifunctional protein both and chromosome partition.