A5080640564- Cystic Fibrosis Research Advances
- Vitamin D Research Studies
- Transplantation: Methods and Outcomes
- Neonatal Respiratory Health Research
- SARS-CoV-2 and COVID-19 Research
- Cholesterol and Lipid Metabolism
- Child Nutrition and Feeding Issues
- Vitamin C and Antioxidants Research
- Iron Metabolism and Disorders
- Organ Transplantation Techniques and Outcomes
- Dysphagia Assessment and Management
- Pharmacological Effects and Toxicity Studies
- Trace Elements in Health
- Hemoglobinopathies and Related Disorders
- Infant Nutrition and Health
- Peroxisome Proliferator-Activated Receptors
- SARS-CoV-2 detection and testing
- Drug Transport and Resistance Mechanisms
- Pancreatitis Pathology and Treatment
- COVID-19 Clinical Research Studies
- Asthma and respiratory diseases
- Health, Medicine and Society
- Bone Metabolism and Diseases
- Breastfeeding Practices and Influences
- HIV Research and Treatment
Lunenfeld-Tanenbaum Research Institute
2023-2025
Université de Montréal
2015-2024
Centre Hospitalier Universitaire Sainte-Justine
2015-2024
Princess Margaret Cancer Centre
2024
University Health Network
2024
University of Toronto
2023
Calgary Laboratory Services
2023
Sinai Health System
2022
Université Laval
2017
Heinrich Heine University Düsseldorf
2015
Growing evidence suggests that the small intestine may contribute to excessive postprandial lipemia, which is highly prevalent in insulin-resistant/Type 2 diabetic individuals and substantially increases risk of cardiovascular disease. The aim present study was determine role high glucose levels on intestinal cholesterol absorption, transporter expression, enzymes controlling homeostasis, status transcription factors. To this end, we employed differentiated polarized cells (20 days culture),...
Cystic fibrosis (CF)-related diabetes (CFRD) has become a critical complication that seriously affects the clinical outcomes of CF patients. Although CFRD emerged as most common nonpulmonary CF, little is known about its etiopathogenesis. Additionally, whether oxidative stress (OxS), feature and diabetes, influences pathophysiology requires clarification. The main objective this study was to shed light on role cystic transmembrane conductance regulator (CFTR) in combination with OxS insulin...
Trials in school-aged children suggest vitamin D supplementation reduces asthma exacerbations. Primary aim: to examine whether D3 (100,000 IU) rapidly raises serum 25-hydroxyvitamin (25OHD) ≥75 nmol/L asthmatic preschoolers.In a double-blind, randomised, placebo-controlled trial, preschool-aged with received 100,000 IU (intervention) or placebo (control), followed by 400 daily for 6 months. Serum 25OHD was measured at baseline, 10 days, 3 and Outcomes included the group difference change...
Older individuals and people with HIV (PWH) were prioritized for COVID-19 vaccination, yet comprehensive studies of the immunogenicity these vaccines their effects on reservoirs are not available. Our study 68 PWH 23 HIV-negative participants aged 55 older post-three vaccine doses showed equally strong anti-spike IgG responses in serum saliva through week 48 from baseline, while salivary IgA low. had diminished live-virus neutralization after two doses, which 'rescued' post-booster....
Abstract Background The protection afforded by COVID-19 vaccination wanes over time and varies SARS-CoV-2 strain. Identifying a marker to serve as an immunologic correlate of (CoP) is crucial ensure future vaccine iterations are sufficiently protective inform recommendations for the timing subsequent doses.Figure 1.Antibody titres.The probability log transformed anti-Spike anti-RBD IgG titres with raw values represented dots. Methods A prospective cohort asymptomatic adults exposed household...
Osteoclasts differentiate from hematopoietic mononuclear precursor cells under the control of both colony stimulating factor-1 (CSF-1, or M-CSF) and receptor activator NF-κB ligand (RANKL, TRANCE, TNFSF11) to carry out bone resorption. Using high density gene microarrays, we followed expression changes in long RNA when CSF-1 injections were used restore osteoclast populations CSF-1-null toothless (csf1tl/csf1tl) osteopetrotic rat. We found that ovarian cancer G-protein-coupled 1 (OGR1,...
BACKGROUND AND OBJECTIVES: Recent data suggest that cow’s milk allergy (CMA) has become more persistent, prolonging treatment via strict elimination of products into a period skeletal growth. The objectives this study were to compare bone mineral density (BMD), vitamin D status, and dietary intakes calcium between prepubertal children with persistent CMA those non–cow’s food allergies (NCMA) as control subjects assess the use compliance supplementation among CMA. METHODS: Fifty-two 29 NCMA...
The role of intestinal fatty acid binding protein (I-FABP) in lipid metabolism remains elusive. To address this issue, normal human epithelial cells (HIEC-6) were transfected with cDNA to overexpress I-FABP and compared treated empty pQCXIP vector. overexpression stimulated mitochondrial [U-14C]oleate oxidation CO2 acid-soluble metabolites via mechanisms including the upregulation expression activity carnitine palmitoyltransferase 1, a critical enzyme controlling entry (FA) into...
Cystic fibrosis-related diabetes is primarily due to a defect in insulin secretion. Women with cystic fibrosis (CF) are at higher risk of developing CF-related diabetes.The objective the study was examine sex differences and glucose homeostasis. We hypothesized that CF, women would display lower secretion than men.This based on an ongoing observational CF cohort mean follow-up 19.9 ± 5.2 months.The conducted clinic Centre Hospitalier de l'Université Montréal (Québec, Canada).From 230 adults...
Hyperinflammation is a hallmark feature of cystic fibrosis (CF) airways. However, inflammation has also been documented systemically and, more recently, in extrapulmonary CF-affected tissues such as the pancreas and intestine. The pathogenesis CF-related specifically role transmembrane conductance regulator (CFTR) that respect are not entirely understood. We have tested hypothesis genetic depletion CFTR will affect inflammatory status human intestinal epithelial cell lines.CFTR expression...
Abstract Bone morphogenetic proteins (BMPs) play pivotal roles in bone and cartilage growth repair. Through phenotypes of short ‐ ear ( se ) mice, which have BMP‐5 mutations, a role for some specific aspects skeletogenesis is known. This report examines expression the plate differentiating cultures primary chondrocytes, effects addition or its inhibition by anti‐BMP‐5 antibody chondrocyte cultures. By laser capture microdissection immunohistochemistry, we found that expressed proliferating...
Background Although mitochondrial dysfunction and oxidative stress are central mechanisms in various pathological conditions, they have not been extensively studied the gastrointestinal tract, which is known to be constantly exposed luminal oxidants from ingested foods. Key among these simultaneous consumption of iron salts ascorbic acid, can cause damage biomolecules. Methodology/Principal Findings The objective present work was evaluate how iron-ascorbate (FE/ASC)-mediated lipid...
New evidence supports the use of supplemental vitamin D in prevention exacerbation asthma; however, optimal posology to sufficiently raise serum levels while maximising adherence is unclear. The objective was ascertain efficacy high-dose D3 increasing preschoolers with asthma and provide preliminary data on safety outcomes. We conducted a 7-month, triple-blind, randomised, placebo-controlled, pilot trial children aged 1–5 years viral-induced asthma. Participants were allocated receive two...
Background Abnormal fatty acid composition (FA) in plasma and tissue lipids frequently occurs homozygous even heterozygous carriers of cystic fibrosis transmembrane conductance regulator (CFTR) mutations. The mechanism(s) underlying these abnormalities remained, however, poorly understood despite the potentially CFTR contributing role. Methodology/Principal Findings aim present study was to investigate impact depletion on FA uptake, metabolism using intestinal Caco-2/15 cell line....
Cystic fibrosis transmembrane conductance regulator (CFTR) is a chloride channel highly expressed in epithelial cells of the gastrointestinal tract. Mutations CFTR gene cause cystic (CF), disease characterized by pancreatic insufficiency, fat malabsorption, and steatorrhea. Despite administration enzymes to normalize CF patients still experienced lipid fecal loss, nutritional deficiencies, abnormalities serum profile, suggesting presence intrinsic defects intestinal handling nutrients. The...
Escherichia coli β-galactosidase (β-gal), encoded by the lacZ gene, has become an essential tool in studies of gene expression and function higher eukaryotes. lac-Z is widely used as a marker to detect transgenes or Cre recombinase driven tissue-specific promoters. The timing location promoter activity easily visualized whole embryos specific tissues using cleavable, chromogenic substrate, 5-bromo-4-chloro-3-indolyl-D-galactopyranoside (X-gal). tissue specificity promoters transgenic...
Obesity and underweight are associated with a higher postlung transplantation (LTx) mortality. This study aims to assess the impact of changes in body mass index (BMI) during waiting period for LTx on early postoperative outcomes.Medical records 502 consecutive cases performed at our institution between 1999 2015 were reviewed. Patients stratified per change BMI category pre-LTx assessment (candidate BMI) transplant as follows: A-candidate BMI, less than 18.5 or 29.9 18.5; B-candidate 29.9;...