A5020092026- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Aquaculture Nutrition and Growth
- Crustacean biology and ecology
- Reproductive biology and impacts on aquatic species
- Neurobiology and Insect Physiology Research
- Animal Genetics and Reproduction
- Genetic diversity and population structure
- Invertebrate Immune Response Mechanisms
- MicroRNA in disease regulation
- Fish Biology and Ecology Studies
- Epigenetics and DNA Methylation
- Chromosomal and Genetic Variations
- Reproductive Biology and Fertility
- Hypothalamic control of reproductive hormones
- Insect Resistance and Genetics
- Genetics, Bioinformatics, and Biomedical Research
- Protist diversity and phylogeny
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Microtubule and mitosis dynamics
- Genomics and Phylogenetic Studies
- Genetics, Aging, and Longevity in Model Organisms
- Aquaculture disease management and microbiota
- Identification and Quantification in Food
- Meat and Animal Product Quality
Shanghai Ocean University
2014-2024
Ministry of Agriculture and Rural Affairs
2020-2023
Shanghai Municipal Education Commission
2011
Ministère de l'Agriculture et de la Souveraineté alimentaire
2011
Ministry of Agriculture & Farmers Welfare
2011
Japan Fisheries Research and Education Agency
2003-2009
West Virginia University
2007
National Center for Cool and Cold Water Aquaculture
2007
Feng Chia University
1997
Background The oriental river prawn, Macrobrachium nipponense, is an economically and nutritionally important species of the Palaemonidae family decapod crustaceans. To date, sequencing its whole genome unavailable as a non-model organism. Transcriptomic information also scarce for this species. In study, we performed de novo transcriptome to produce first comprehensive expressed sequence tag (EST) dataset M. nipponense using high-throughput technologies. Methodology Principal Findings Total...
MicroRNAs (miRNAs) are small non-coding RNA molecules that downregulate gene expression by base pairing to the 3′-untranslated region (UTR) of target messenger RNAs (mRNAs). Up now, rare information for miRNAs is available in decapod crustaceans. Our previous studies showed many miRNA-binding sites present 3′-UTR cyclin B Chinese mitten crab Eriocheir sinensis, suggesting translation or post-transcription might be regulated during meiosis oocyte. To identify ovarian crab, were subjected...
As an important freshwater aquaculture species in China, the Chinese mitten crab (Eriocheir japonica sinensis) has high economic and nutritional value. However, limited genomic information is currently available for studying its basic development genetic diversity. Here, we performed whole-genome sequencing on Oxford Nanopore Technologies Limited's platform using promethION. The assembled size of E. j.sinensis genome was approximately 1.27 Gb, which close to estimated (1.19 Gb). Furthermore,...
During recent years, China has become a hotspot for the domestication of mandarin fish, and this is great commercial value. Although food preference domesticated fish been studied, little known about genes regulating their growth. We raised hybrid on artificial feed 3 months, results showed that survival rate was 60.00%. Their total length body weight were 18.34 ±0.43 cm 100.44 ±4.87 g. The absolute gain rates 0.14 cm/d 1.08 g/d, respectively. Finally, RNA sequencing (RNA-Seq) performed to...
In penaeid shrimp, cortical rods (CRs) are formed in peripheral crypts of the oocyte after completion yolk accumulation; subsequently CRs utilized as a source jelly materials that surround fertilized eggs. our previous study, five major components, three CR proteins displayed quite similar immunological characteristics. this cDNA sequences and developmental expression profiles at both transcriptional protein levels were examined to elucidate molecular characteristics process formation....
Summary Sex determination mechanisms in many crustacean species are complex and poorly documented. In the giant freshwater prawn, Macrobrachium rosenbergii , a ZW / ZZ sex system was previously proposed based on ratio data obtained by crosses of sex‐reversed females (neomales). To provide molecular evidence for system, novel sex‐linked markers were isolated this species. Amplified fragment length polymorphism ( AFLP ) using 64 primer combinations employed to screen prawn genomes DNA linked...
Microsatellites are simple sequence repeats with a high degree of polymorphism in the genome; they used as DNA markers many molecular genetic studies. Using traditional methods such magnetic beads enrichment method, only few microsatellite have been isolated from Chinese mitten crab Eriocheir sinensis, genome information is unavailable. Here, we identified large number microsatellites by taking advantage Solexa genomic surveying. A total 141,737 SSR (simple repeats) motifs were via analysis...
Abstract SRY -related HMG box ( Sox ) genes are characterized by the presence of a DNA-binding domain and involved in diverse range developmental processes. In this study, we identified novel gene, designated as EsSoxB2-1 , from Chinese mitten crab Eriocheir sinensis . The encodes protein 259 amino acids, sharing highest identity with beetle Tribolium castaneum SOX21b. Unlike insect Sox21b however, is intronless exhibits gonad-specific expression pattern at both mRNA level. Two core...
As post-transcriptional regulators, microRNAs (miRNAs) play an important role in growth and reproductive processes. So far, there is limited information regarding crustacean miRNAs. To explore the potential of miRNAs gonadal development prawn Macrobrachium rosenbergii, we constructed seven small RNA libraries from ovarian testicular tissues at various stages using somatic tissue as control. A total 1,954 known 129 novel were retrieved. By comparing differentially expressed (DEMs) between...