A5103207044- CRISPR and Genetic Engineering
- Genetically Modified Organisms Research
- Genomics and Phylogenetic Studies
- Insect Resistance and Genetics
- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Microbial Metabolic Engineering and Bioproduction
- Crustacean biology and ecology
- Cancer-related molecular mechanisms research
- Plant biochemistry and biosynthesis
- Rheumatoid Arthritis Research and Therapies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- MicroRNA in disease regulation
- Enzyme Production and Characterization
- Invertebrate Immune Response Mechanisms
- Photosynthetic Processes and Mechanisms
- Medicinal Plant Pharmacodynamics Research
- Enzyme Catalysis and Immobilization
- Protein Hydrolysis and Bioactive Peptides
- Microbial Metabolism and Applications
- RNA Research and Splicing
- Chromosomal and Genetic Variations
- Photochromic and Fluorescence Chemistry
- Dyeing and Modifying Textile Fibers
- Fish biology, ecology, and behavior
Beijing Forestry University
2023-2025
Northeast Normal University
2025
Anhui University of Traditional Chinese Medicine
2020-2025
Zhejiang University
2022-2024
Shihezi University
2024
Sichuan Agricultural University
2024
Gannan Normal University
2021-2023
Jiangxi Agricultural University
2020-2023
Jilin Medical University
2022-2023
Jilin University
2022-2023
Comprehensive utilization of cottonseeds is limited by the presence pigment glands and its inclusion gossypol. The ideal cotton has glandless seeds but a glanded plant, trait found in only few Australian wild species, including Gossypium bickii. Introgression this into cultivated species proved to be difficult. Understanding biological processes toward gland morphogenesis associated underlying molecular mechanisms will facilitate breeding varieties with plant. In study, single-cell RNA...
Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by synovial inflammation and progressive joint destruction. Neutrophil extracellular traps (NETs), microreticular structure formed after neutrophil death, have recently been implicated in RA pathogenesis pathological mechanisms. However, the underlying molecular mechanisms key genes involved NET formation remain largely unknown. We obtained single-cell RNA sequencing data of tissues from Gene Expression Omnibus (GEO)...
Abstract Background The swimming crab, Portunus trituberculatus, is an important commercial species in China and widely distributed the coastal waters of Asia-Pacific countries. Despite increasing interest crab research, a high-quality chromosome-level genome still lacking. Findings Here, we assembled first reference P. trituberculatus by combining short reads, Nanopore long Hi-C data. assembly size was 1.00 Gb with contig N50 length 4.12 Mb. In addition, BUSCO assessment indicated that...
Circular RNAs (circRNAs) are a significant class of molecules involved in wide range diverse biological functions that abnormally expressed many types diseases. The present study aimed to determine the circRNAs specifically peripheral blood mononuclear cells (PBMCs) from rheumatoid arthritis (RA) patients identify their possible molecular mechanisms.To RA, we started by sequencing PBMCs circRNA and microRNAs (miRNAs) RA group (n = 3) control 3). We constructed network differentially miRNAs....
As an important freshwater aquaculture species in China, the Chinese mitten crab (Eriocheir japonica sinensis) has high economic and nutritional value. However, limited genomic information is currently available for studying its basic development genetic diversity. Here, we performed whole-genome sequencing on Oxford Nanopore Technologies Limited's platform using promethION. The assembled size of E. j.sinensis genome was approximately 1.27 Gb, which close to estimated (1.19 Gb). Furthermore,...
<title>Abstract</title> Crabs encompass the infra-orders Brachyura and Anomura, collectively constitute clade Meiura within order Decapoda. Despite their considerable diversity, genomic resources for crabs remain scarce, hindering our understanding of phylogeny genetic mechanisms underlying such unique traits as carcinization. To address these questions, here we sequenced genomes 10 crab species covering all currently controversial taxonomies at section level. Our whole-genome phylogenetic...
Light-dependent enzymes are a rare type of biocatalyst with high potential for research and biotechnology. A recently discovered fatty acid photo-decarboxylase from Chlorella variabilis NC64A (CvFAP) converts acids to the corresponding hydrocarbons only when irradiated blue light (400 520 nm). To expand available catalytic diversity decarboxylation, we reconstructed possible ancestral decarboxylases set 12 extant sequences that were classified under clade within glucose-methanol choline...
Golden leaf in autumn is a prominent feature of deciduous tree species like Ginkgo biloba L., landscape widely cultivated worldwide. However, little was known about the molecular mechanisms yellowing, especially its dynamic regulatory network. Here, we performed suite comparative physiological and transcriptional analyses on golden-leaf cultivar wild type (WT) ginkgo to investigate underlying yellowing across different seasons.In present study, used natural bud mutant with yellow leaves...
Abstract The blue king crab, Paralithodes platypus , which belongs to the family Lithodidae, is a commercially and ecologically important species. However, high‐quality reference genome for crab has not yet been reported. Here, we assembled first chromosome‐level genome, contains 104 chromosomes an N50 length of 51.15 Mb. Furthermore, determined that large size can be attributed insertion long interspersed nuclear elements tandem repeats. Genome assembly assessment showed 96.54% transcripts...
Paeonia ludlowii (Stern & G. Taylor D.Y. Hong) belongs to the peony group of genus in Paeoniaceae family and is now classified as a "critically endangered species" China. Reproduction important for this species, its low fruiting rate has become critical factor limiting both expansion wild population domestic cultivation.In study, we investigated possible causes ovule abortion ludlowii. We clarified characteristics specific time ludlowii, used transcriptome sequencing investigate mechanism...
Abstract τ-Cadinol is a sesquiterpene that widely used in perfume, fine chemicals and medicines industry. In this study, we established biosynthetic pathway for the first time engineered Escherichia coli production of τ-cadinol from simple carbon sources. Subsequently, further improved to 35.9 ± 4.3 mg/L by optimizing overproduction rate-limiting enzyme IdI. Finally, titer was increased 133.5 11.2 with two-phase organic overlay-culture medium system. This study shows an efficient method...
Abstract Benzylisoquinoline alkaloids (BIAs) are an important class of plant secondary metabolites with a variety pharmacological activities. Although they widely used, traditionally these compounds extracted from natural sources because their structure is too complicated to achieve economically feasible chemical synthesis. Thus, microbial biosynthesis BIAs expected reduce dependence on extracts. ( S )‐Reticuline precursor for biosynthesis. Therefore, it attractive engineering target. In...
Alzheimer's disease (AD) is a neurodegenerative disorder characterized by progressive memory loss and cognitive dysfunction. Quinone oxidoreductase 1 (NQO1) an antioxidant enzyme that plays important role in controlling cellular redox state, whose expression altered the brain tissues of AD patients. In addition to its traditional effects, NQO1 also acts as multifunctional RNA-binding protein involved posttranscriptional regulation. Whether activity influences pathology has not been...
Abstract Blood orange (BO) is a rare red-fleshed sweet (SWO) with high anthocyanin content and associated numerous health-related benefits. Here, we reported high-quality chromosome-scale genome assembly for Neixiu (NX) BO, reaching 336.63 Mb in length contig scaffold N50 values of 30.6 Mb. Furthermore, 96% the assembled sequences were successfully anchored to 9 pseudo-chromosomes. The also revealed presence 37.87% transposon elements 7.64% tandem repeats, annotation 30,395 protein-coding...
Parkinson's disease (PD) involves multiple forms of neuronal cell death, but the dominant pathway involved in progression remains unclear. This study employed RNA sequencing (RNA-seq) brain tissue to explore gene expression patterns across different stages PD. Using Scaden deep learning algorithm, we predicted neurocyte subtypes and modelled dynamic interactions for five classic death pathways identify predominant routes during PD progression. Our type-specific analysis revealed an...
Twenty-one RNA minihelices, resembling the coaxially stacked acceptor- /T-stems and T-loop found along top of a tRNA's three-dimensional structure, were synthesized used as substrates for ATP/CTP:tRNA nucleotidyltransferases from Escherichia coli Saccharomyces cerevisiae. The sequence nucleotides in loop varied at positions corresponding to residues 56, 57 58 tRNA. All minihelices both enzymes, identity bases affected interaction. In general, RNAs with purines better than those pyrimidines,...
In order to enrich the Anser cygnoides genome and identify gene expression profiles of primary secondary feather follicles development, de novo transcriptome assembly skin tissues was established by analyzing three developmental stages at embryonic day 14, 18, 28 (E14, E18, E28). Sequencing output generated 436,730,608 clean reads from nine libraries assembled into 56,301 unigenes. There were 2,298, 9,423 12,559 unigenes showing differential in respectively. Furthermore, differentially...