A5108049284- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- RNA Interference and Gene Delivery
- Neuroinflammation and Neurodegeneration Mechanisms
- Acupuncture Treatment Research Studies
- Tryptophan and brain disorders
- Neural dynamics and brain function
- Genetics and Neurodevelopmental Disorders
- Stress Responses and Cortisol
- Nerve injury and regeneration
- Signaling Pathways in Disease
- Parkinson's Disease Mechanisms and Treatments
- Virus-based gene therapy research
- Neuroscience and Neural Engineering
- Ion channel regulation and function
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Neurological Disorders and Treatments
- Neuroendocrine regulation and behavior
- Histone Deacetylase Inhibitors Research
- Calcium signaling and nucleotide metabolism
- Nuclear Receptors and Signaling
- Neurological Disease Mechanisms and Treatments
- Calpain Protease Function and Regulation
- Sirtuins and Resveratrol in Medicine
Shanghai Jiao Tong University
2014-2024
Bridge University
2023
Development Research Center
2023
BioMed X Institute
2022
Shanghai Mental Health Center
2022
The University of Tokyo
1999-2005
Okayama University
2002-2004
Japan Science and Technology Agency
2000-2002
Tokyo University of Science
1999-2000
RIKEN
1999
Calcineurin and calpain, a Ca2+/calmodulin-dependent protein phosphatase Ca2+-dependent cysteine protease, respectively, mediate neuronal cell death through independent cascades. Here, we report that during neuroexcitotoxicity, calcineurin A (CnA) is directly cleaved by calpain in vitro vivo, resulting the enzyme being converted to an active form. Mass spectrometry identified three cleavage sites CnA, two of which were constitutively forms. Overexpression CnA induced caspase activity death....
Cyclin-dependent kinase 5 (Cdk5) is a proline-directed serine/threonine with close structural homology to the mitotic Cdks. The complex of Cdk5 and p35, neuron-specific regulatory subunit Cdk5, plays important roles in brain development, such as neuronal migration neurite outgrowth. Moreover, thought be involved promotion neurodegeneration Alzheimer's disease. abundant mature neurons; however, its physiological functions adult are unknown. Here we show that Cdk5/p35 regulates...
It has been thought that clathrin-mediated endocytosis is regulated by phosphorylation and dephosphorylation of many endocytic proteins, including amphiphysin I dynamin I. Here, we show Cdk5/p35-dependent cophosphorylation plays a critical role in such processes. Cdk5 inhibitors enhanced the electric stimulation–induced hippocampal neurons, was also neurons p35-deficient mice. phosphorylated proline-rich domain both vitro vivo. Cdk5-dependent inhibited association with β-adaptin....
Obesity is associated with a high prevalence of mood disorders such as anxiety and depression. Both stress fat diet can alter the gut microbiota contribute to obesity. To examine interrelationships between obesity, stress, disorders, obesity was induced in mice using diet, were subsequently stressed chronic unpredictable mild protocol. During experiment, composition analyzed by 16 S rRNA gene high-throughput sequencing, anxiety-like behaviors measured. The results revealed distinct gender...
Proteins and peptides have been demonstrated to penetrate across the plasma membrane of eukaryotic cells by protein transduction domains. We show that 11 arginine (<i>11R</i>) is an efficient method delivering proteins into neurons brain slices. Here, we demonstrate PKA inhibitory peptide, fused with <i>11R</i> nuclear localization signal, delivers peptide exclusively compartment in This blocked both cAMP responsive element-binding phosphorylation long-lasting long-term potentiation (LTP)...
Objective: Transcranial ultrasound stimulation (TUS) is a noninvasive neuromodulation technique with good spatial resolution and deep penetration. This study aims to investigate whether TUS has antidepressant-like effect depressed rats. Methods: Rats were divided into five groups, including two groups (ST-Ctr ST-Res) for evaluating the short-term impact of restraint stress three (LT-Ctr-ShamTUS, LT-Res-ShamTUS LT-Res-TUS) studying long-term effects stimulation. The TUS-treated rats subjected...
Alzheimer's disease (AD) is the most common neurodegenerative characterized by accumulation of amyloid β peptides (Aβ) and impaired glucose metabolism in brain. Osteocalcin (OCN), an osteoblast-derived protein, has been shown to modulate brain functions but whether it any effect on AD undetermined. In this study, daily intraperitoneal injection OCN for 4 weeks ameliorated anxiety-like behaviors cognitive dysfunctions APP/PS1 transgenic mice model, as increased entries into central area open...
This study focused on a promising drug candidate, N-[2-(3,4-dimethoxyphenyl)ethyl]-3-phenyl-acrylamide (gx-50), compound extracted from Sichuan pepper (Zanthoxylum Bungeanum), to determine whether it would be an effective therapeutic for Alzheimer's disease (AD) via biological experiments. In vivo, we determined the pharmacokinetic profile of gx-50 and evaluated effect cognitive abilities amyloid-β protein precursor transgenic (AβPP-Tg) mice by Morris water maze testing. addition, examined...
Epigenomic abnormalities caused by genetic mutation in epigenetic regulators can result neurodevelopmental disorders, deficiency neural plasticity and mental retardation. As a histone demethylase, plant homeodomain finger protein 8 (Phf8) is candidate gene for syndromal non-specific forms of X-chromosome-linked intellectual disability (XLID). Here we report that Phf8 knockout mice displayed impaired learning memory, hippocampal long-term potentiation (LTP) without gross morphological...
The protein transduction system has been employed for delivery of bioactive proteins into cells via an endocytotic mechanism. However, trapping endocytosed in the endosome may significantly attenuate biological actions cells. present investigation demonstrated that endosomal release transduced could be artificially accelerated by exposure to fluorescent light. Exposure light at 480 nm stimulated FITC‐11 arginine‐protein domain (11R‐PTD), TAT‐PTD and Antennapedia‐PTD. Moreover, FITC‐11R‐p53...
Osteoblasts derived osteocalcin (OCN) is recently reported to be involved in dopaminergic neuronal development. As injury the substantia nigra (SN) a pathological hallmark of Parkinson's disease (PD), we investigated whether OCN could exert protective effects on 6-hydroxydopamine (6-OHDA)-induced PD rat model. Our data showed that level cerebrospinal fluid models was significantly lower than controls. Intervention with improve behavioral dysfunction and reduce tyrosine hydroxylase loss...
Abstract Although memory deficits have been clearly documented in patients with human immunodeficiency virus type‐1 (HIV‐1) infection, the physiological basis of this dysfunction is poorly understood. We focused on Tat, a viral protein released from HIV‐1–infected cells and investigated its effect spatial learning adult mice. An intracerebroventricular injection Tat leads to attenuation accompanied by suppression long‐term potentiation (LTP), cellular learning, hippocampal cornu ammonis 1...
Abstract In the adult mouse hippocampus, NMDA receptors (NMDARs) of CA1 neurons play an important role in synaptic plasticity. The location NMDARs can determine their roles induction long-term potentiation (LTP). However, extrasynaptic (ES-NMDARs) dependent LTP haven’t been reported. Here, through use a 5-Hz stimulation and MK-801 (an irreversible antagonist NMDARs) mice hippocampal slices, were selectively inhibited NMDAR-mediated excitatory postsynaptic currents not recovered. We found...
N6-methyladenosine (m6A) modification controls cell fate determination. Here, we show that liquid-liquid phase separation (LLPS) of YTH RNA binding protein 1 (YTHDF1), a pivotal m6A "reader" protein, promotes the transdifferentiation spermatogonial stem cells (SSCs) into neural cell-like by activating IκB-nuclear factor κB (NF-κB)-CCND1 axis. The inhibition IκBα/β mRNA translation mediated YTHDF1 LLPS is key to activation IκB-NF-κB-CCND1 Disrupting either or NF-κB inhibits efficiency....
We investigated metabotropic glutamate receptor (mGluR)-dependent long-term depression (LTD) in hippocampal CA1 pyramidal neurons of 6- to 8-d-old [postnatal days 6-8 (P6-P8)] and 21- 25-d-old (P21-P25) rats. In P6-P8 rats, induction LTD depended on the activity group II mGluRs. P21-P25 however, this disappeared, instead, NMDA (NMDAR)-dependent appeared. A bath containing a specific calcineurin (CaN) inhibitor restored mGluR-dependent Although postsynaptic injection CaN inhibitors suppressed...