A5013623451- Respiratory viral infections research
- Neonatal Respiratory Health Research
- Pneumonia and Respiratory Infections
- Congenital Diaphragmatic Hernia Studies
- Microbial infections and disease research
- Bacterial Infections and Vaccines
- Tracheal and airway disorders
- Respiratory and Cough-Related Research
- Streptococcal Infections and Treatments
- Pediatric health and respiratory diseases
- Influenza Virus Research Studies
- Immune Cell Function and Interaction
- Transplantation: Methods and Outcomes
- Genetic Syndromes and Imprinting
- Bacteriophages and microbial interactions
- Dermatology and Skin Diseases
- Heparin-Induced Thrombocytopenia and Thrombosis
- Nitric Oxide and Endothelin Effects
- Infectious Encephalopathies and Encephalitis
- Medical Imaging and Pathology Studies
- Asthma and respiratory diseases
- Genetics and Neurodevelopmental Disorders
- Parvovirus B19 Infection Studies
- interferon and immune responses
- Platelet Disorders and Treatments
Jinshan Hospital of Fudan University
2025
Children's Hospital of Fudan University
2016-2025
University of Hong Kong
2016
Fudan University
2014-2015
<h3>BACKGROUND:</h3> Corticosteroids have been used for refractory <i>Mycoplasma pneumoniae</i> pneumonia and beneficial effects. The aim of this study was to identify the biomarkers predicting <i>M. in a timely fashion initiate steroid therapy. <h3>METHODS:</h3> This prospective cohort children with admitted Children9s Hospital Fudan University from September 2012 August 2013. Lactate dehydrogenase (LDH) other laboratory tests, including complete blood counts, C-reactive protein,...
Introduction Little was known about the characteristics of low respiratory tract (LRT) microbiota refractory M. pneumoniae pneumonia (RMPP) in children before and after COVID-19 pandemic. Methods Forty-two diagnosed with RMPP 2019 (Y2019 group) 33 2023 (Y2023 group), entered into study. The clinical findings were examined, LRT analyzed by metagenomic next generation sequencing. Results ratio consolidate, atelectasis, lung necrosis, erythema multiforme Y2023 group significantly higher than...
ABSTRACT Pneumonia is the leading cause of morbidity and mortality in children needs rapid accurate pathogenic diagnosis. The aim this study was to evaluate diagnostic value bronchoalveolar lavage fluid (BALF) metagenomic next-generation sequencing (mNGS) conventional microbiological tests (CMTs) for pathogen detection with severe or refractory pneumonia. In retrospective study, clinical data 127 pneumonia admitted respiratory department from June 2021 March 2022 were analyzed. BALF mNGS...
There is a recent global surge in Mycoplasma pneumoniae pneumonia (MPP). However, the key immune factors that contribute to advancement of disease remain unknown. Hence, we conducted this study uncover immunological profile children affected by MPP. This enrolled visiting Children's Hospital Fudan University from December 2023 April 2024, including 34 healthy controls, 51 severe MPP (S-MPP), 27 non-severe (NS-MPP), and non-MPP (NMP) cases. Their blood samples were analyzed using flow...
To evaluate the efficacy of combined treatment ciprofloxacin and glucocorticoid for child refractory Mycoplasma pneumoniae (M. pneumoniae) pneumonia.Clinical laboratory characteristics six pediatric M. pneumonia cases treated with glucocorticoids were reported.Five complicated pleural effusion. The average febrile period prior to admission was 8.5 ± 2.0 days, total 14.6 7.6 after 3.3 3.0 days. time in hospital patients 23.6 4.8 initial mean WBC count 10,100 2,400/mm(3) . erythrocyte...
The role of endothelial nitric oxide synthase (eNOS) in the activities progenitor cells (EPCs) including migration, proliferation, and tube formation vitro was investigated. EPCs were obtained from rat bone mononuclear by culturing for 7-10 days EGM-2MV identified their capacity FITC-UEA-1 binding acetylated low-density lipoprotein (Dil-ac-LDL) intake using fluorescence microscopy. Migration, proliferation assessed presence or absence N(ω)-nitro-L-argininemethylester (L-NAME), an eNOS...
Background: Paracrine factors secreted by endothelial progenitor cells (EPCs) are suggested to be responsible, in part, for the improved microvascular development bronchopulmonary dysplasia (BPD) models. This study aims investigate potential role of exosomes derived from EPCs (EPC-EXOs), a component paracrine secretion, angiogenesis mediating activity PMVECs exposed hyperoxia. Methods: were isolated bone marrow rats. EPC-EXOs ExoQuick-TC kits conditioned media EPCs. The divided into three...
BackgroundThe incidence and mortality of influenza in children had risen, but data are limited on with severe virus infection China.MethodsWe conducted a retrospective case–control study collected the patients' clinical data. Clinical including demography, presentation, laboratory findings, radiologic treatment outcomes were collected. Children clinically confirmed to have Shanghai three hospitals from June 2014 2019.ResultsDuring study, 36,047 enrolled. Among them, 118 met criteria for flu....
Background. Both M. pneumoniae and human adenovirus (HAdV) are common causative agents of lower respiratory tract infection in children; nonetheless, the lung microbiota patients with coinfection HAdV remain unexplored. Methods. Thirty-two children, diagnosed refractory pneumonia (RMPP), entered into one-year study from July 1, 2019 to June 30, 2020. Among them, twenty-one monoinfection (MP) group eleven (MP&ADV) group. The characteristics clinical findings were examined, was analyzed by...
Background: The accuracy of nasopharyngeal aspirate (NPA) specimens in detecting lower respiratory pathogens remains controversial. objective this study was to evaluate the diagnostic aspirates (NPAs) specimen tract infections (LRTIs) children. Methods: prospective designed collect data paired NPAs and bronchoalveolar lavage fluids from children with acute LRTIs January 2013 December 2015. All were subjected pathogen detection: bacterial detection by culture, Mycoplasma pneumoniae (Mp)...
Sleep-related breathing disorders are common in individuals with Prader-Willi syndrome (PWS), and can include hypersomnolence obstructive sleep apnea, as well central abnormalities that present from infancy. Here we describe the sleep-disordered (SDB) genetic findings patients PWS China.In all, 48 confirmed by tests were enrolled, 32 under 2 years of age 16 older children. There 37 (77.1%) paternal 15q11-13 deletions, 11 (22.9%) maternal uniparental disomy (mUPD), no imprinting defect...
Highly homogeneous microbial communities dominated by Mycoplasma pneumoniae instead of increased resistance to macrolide antibiotics is the characteristic lower respiratory tract microbiome children with refractory pneumonia
Aim Respiratory syncytial virus ( RSV ) infection is a major health problem in young children. This study aimed to investigate whether the interleukin IL )‐4 −590 C/T and −33 polymorphisms were associated with susceptibility Chinese Han children severity of infection. Methods The ‐4 SNPs evaluated 218 inpatients bronchiolitis 303 healthy controls. was using respiratory scoring system. Results frequencies CC , CT TT patients versus controls 1.4% 1.7%, 21.1% 34% 77.5% 64.4%, respectively....
IFN-γ, an essential cytokine in the viral cell-mediated immune response, has been associated with pathogenesis of respiratory syncytial virus (RSV) bronchiolitis and to severity infection. The aim this study was investigate whether IFN-γ CA microsatellite (rs3138557) polymorphism susceptibility RSV Chinese Han children infection.The tested 218 inpatients 303 healthy controls, evaluated using a standardised scoring system.The frequencies CA12+/CA12+, CA12+/CA12- CA12-/CA12- patients controls...
It is well documented that vibration techniques and positive expiratory pressure (PEP) can improve airway clearance, however, few clinical trials have studied the efficacy of vibrating PEP therapy on refractory Mycoplasma pneumoniae (M. pneumoniae) pneumonia (RMPP) in children. This study aimed to explore using children with RMPP.Sixty participants remission stage RMPP were recruited into this randomized divided two groups. The Acapella group (n=30) used Acapella® Choice twice daily for 2...
Objective To study the effect of transplanted endothelial progenitor cell (EPC) on hyperoxia-induced lung injury in neonatal rats. Methods Rat bone marrow mononuclear cells were cultured growth medium to obtain EPCs, which identified by morphology, phagocytosis and CD34+ analyses. Sixty Sprague-Dawley rats allowed acclimate room air for 24 h after birth, then divided into four groups (15 per group), including group, hyperoxia EPCs transplantation group Nω-nitro-L-arginine methyl ester...
Objective To analyze the clinical, laboratory and radiological features of children with antineutrophil cytoplasmic antibody-associated vasculitis(AAV) respiratory system. Methods Twelve AAV who were hospitalized from December 2005 to February 2016 in Children′s Hospital Fudan University, descriptive analyzed outpatient clinical data retrospectively analyzed. Results Of 12 children, 9 cases girls 3 boys a mean age 10.35 years old.Eleven categorized as microscopic...