A5040988662- Diabetes and associated disorders
- Mathematical and Theoretical Epidemiology and Ecology Models
- Diabetes Management and Research
- Pancreatic function and diabetes
- T-cell and B-cell Immunology
- Growth Hormone and Insulin-like Growth Factors
- Immune Cell Function and Interaction
- COVID-19 epidemiological studies
- Evolution and Genetic Dynamics
- Cancer-related molecular mechanisms research
- Diet and metabolism studies
- Advanced Differential Equations and Dynamical Systems
- Genetic Syndromes and Imprinting
- Gut microbiota and health
- Ubiquitin and proteasome pathways
- Sexual Differentiation and Disorders
- Adipose Tissue and Metabolism
- Synthesis and Biological Evaluation
- Liver Disease Diagnosis and Treatment
- Nonlinear Dynamics and Pattern Formation
- MicroRNA in disease regulation
- Genetics and Neurodevelopmental Disorders
- Immunodeficiency and Autoimmune Disorders
- Adolescent Sexual and Reproductive Health
- Connective tissue disorders research
Children's Hospital of Fudan University
2015-2024
Guangdong Provincial People's Hospital
2024
Sun Yat-sen University
2017-2023
Key Laboratory of Guangdong Province
2017-2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2023
Peking Union Medical College Hospital
2023
The First Affiliated Hospital, Sun Yat-sen University
2017-2023
Fudan University
2013-2022
Kunming University of Science and Technology
2016-2018
China Railway Corporation
2018
Circular RNAs (circRNAs) are RNA transcripts that widespread in the eukaryotic genome. Recent evidence indicates circRNAs play important roles tissue development, gene regulation, and carcinogenesis. However, whether encode functional proteins remains elusive, although translation of several was recently reported. CircRNA deep sequencing performed by using 10 pathologically diagnosed glioblastoma samples their paired adjacent normal brain tissues. Northern blotting, Sanger sequencing,...
Abstract Gut dysbiosis has been linked to type 1 diabetes (T1D); however, microbial capacity in T1D remains unclear. Here, we integratively profiled gut functional and metabolic alterations children with new-onset independent cohorts investigated the underlying mechanisms. In T1D, microbiota was characterized by decreased butyrate production bile acid metabolism increased lipopolysaccharide biosynthesis at species, gene, metabolite levels. The combination of 18 bacterial species fecal...
Ischemia-reperfusion injury (IRI) has thus far been considered as an inevitable component of organ transplantation, compromising outcomes, and limiting availability. Ischemia-free transplantation is a novel approach designed to avoid IRI, with the potential improve outcomes.
Rationale: Macrophages play critical roles in the pathogenesis of type 1 diabetes mellitus (T1DM). Circular RNAs (circRNAs) are a novel class endogenous with covalently closed loop structures, implicated various disease processes. However, their impact on macrophage activation and T1DM remains elusive. Methods: circRNA expression profiles peripheral blood mononuclear cells (PBMCs) from children were determined by whole transcriptome microarray. Bioinformatics, quantitative real-time PCR,...
OBJECTIVE Type 1 diabetes (T1D) is a highly heritable disease with much lower incidence but more adult-onset cases in the Chinese population. Although genome-wide association studies (GWAS) have identified >60 T1D loci Caucasians, less known Asians. RESEARCH DESIGN AND METHODS We performed first two-stage GWAS of using 2,596 autoantibody-positive case subjects and 5,082 control Han population evaluated associations between risk age fasting C-peptide levels at diagnosis. RESULTS...
Circular RNAs (circRNAs) constitute a class of covalently circular non-coding RNA molecules formed by 5′ and 3′ end back-splicing. The rapid development bioinformatics large-scale sequencing has led to the identification functional circRNAs. Despite an overall upward trend, studies focusing on roles circRNAs in immune diseases remain relatively scarce. In present study, we obtained differential circRNA expression profile based microarray analysis peripheral blood mononuclear cells (PBMCs)...
Blood microbiome signatures in patients with type 1 diabetes (T1D) remain unclear. We profile blood using 16S rRNA gene sequencing 77 controls and 64 children new-onset T1D, compared it the gut oral microbiomes. The of T1D is characterized by increased diversity perturbed microbial features, a significant increase potentially pathogenic bacteria controls. Thirty-six representative genera were identified random forest analysis, providing strong discriminatory power for an AUC 0.82. PICRUSt...
Fatty liver disease is one of the leading causes chronic damage in western countries. Approximately 25% adults United States have fatty livers absence excessive alcohol consumption, a condition termed nonalcoholic (NAFLD). Little known about prevalence and genetic background NAFLD or factors that determine its development. In this study, we used Gene-Cloud Biotechnology Information bioinformatics platform to carry out comprehensive analysis identifying differentially expressed genes (DEGs),...
Lifestyle changes including COVID-19 lockdown cause weight gain and may change obesity trends; however, timely are largely unknown monitoring measures usually lack. This first large-scale study aimed to analyze the real-world national trends of prevalence Chinese children in past five years, impact pandemic on pediatric development through both mobile- hospital-based data. included aged 3 19 years old all over China from January 2017 April 2021. Hospital-measured parent-reported cases XIGAO...
Abstract Objective Killer cell immunoglobulin‐like receptor ( KIR ) genes and their putative ligands human leukocyte antigen (HLA)‐C have been associated with type 1 diabetes (T1D). We hypothesize that HLA‐C are important in T1D aetiology. Research design methods ligand genotyping was performed 259 patients 262 healthy children. Results No significant difference observed the distribution of between controls. However, frequency HLA‐C1 gene HLA‐C2 (marginal association) higher patient group....
Objective Putative changes in the prevalence of obesity Chinese children and adolescents Shanghai, one most urbanized areas China, were analyzed a cross-sectional manner 2003–2008. Methods One urban suburban district randomly selected. 70 582 students 2003 86 355 2008 from schools within two districts examined. Anthropometric parameters measured all. Adiposity status was estimated using body mass index according to International Obesity Task Force standards. The analyzed. Results...
To investigate the function of cytokines, chemokines, and regulatory T cells (Tregs) in pathogenesis type 1 diabetes mellitus (T1DM) children.A total 35 children with T1DM 30 healthy controls were enrolled this study. Levels serum cytokines (IL-1α, IL-6, IL-10, IL-12, TNF-α) chemokines (MIP-1α, MIP-1α MCP-1) detected by enzyme-linked immunosorbent assay. Peripheral blood mononuclear (PBMCs) isolated culture supernatant phytohaemagglutinin (PHA)-stimulated PBMCs was subjected to ELISA for...
Mandibulofacial dysostosis with microcephaly (MFDM) is characteristic of multiple skeletal anomalies comprising craniofacial anomalies/dysplasia, microcephaly, dysplastic ears, choanal atresia, and short stature. Heterozygous loss function variants EFTUD2 was previously reported in MFDM; however, the mechanism underlying EFTUD2-associated dysplasia remains unclear.We identified a novel frameshift variant (c.1030_1031delTG, p.Trp344fs*2) an MFDM Chinese patient dysmorphism including ear canal...