A5028053562- Organ Transplantation Techniques and Outcomes
- Liver Disease and Transplantation
- Renal Transplantation Outcomes and Treatments
- Liver Disease Diagnosis and Treatment
- Transplantation: Methods and Outcomes
- Organ Donation and Transplantation
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Anesthesia and Neurotoxicity Research
- Viral-associated cancers and disorders
- Hepatitis B Virus Studies
- Polyomavirus and related diseases
- Phytoestrogen effects and research
- Circular RNAs in diseases
- Bladder and Urothelial Cancer Treatments
- Kidney Stones and Urolithiasis Treatments
- Cancer, Lipids, and Metabolism
- Chemokine receptors and signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- thermodynamics and calorimetric analyses
- Chemotherapy-induced organ toxicity mitigation
- Ferroptosis and cancer prognosis
- Histiocytic Disorders and Treatments
- Lipid metabolism and disorders
- Phagocytosis and Immune Regulation
Sun Yat-sen University
2017-2025
The First Affiliated Hospital, Sun Yat-sen University
2017-2025
Key Laboratory of Guangdong Province
2017-2025
China International Science and Technology Cooperation
2018-2025
Guangxi Medical University
2022
Saudi Center for Organ Transplantation
2020
Creative Commons
2017
Xiangya Hospital Central South University
2015-2016
Central South University
2015-2016
Flinders Medical Centre
1995
Ischemia-reperfusion injury (IRI) has thus far been considered as an inevitable component of organ transplantation, compromising outcomes, and limiting availability. Ischemia-free transplantation is a novel approach designed to avoid IRI, with the potential improve outcomes.
Ischemia and reperfusion injury (IRI) is an inevitable event in conventional organ transplant procedure associated with significant mortality morbidity post-transplantation. We hypothesize that IRI avoidable if the blood supply for not stopped, thus resulting optimal outcomes. Here we described first case of a novel called ischemia-free transplantation (IFOT) patients end-stage liver disease. The graft severe macrovesicular steatosis was donated from 25-year-old man. recipient 51-year-old...
Immune checkpoint inhibitors (ICIs) may lead to rejection and even graft loss of solid organ transplant recipients, making them not widely used in patients. There is insufficient clinical experience using ICIs as a bridging or downstaging therapy before transplantation. We performed retrospective review patients receiving programmed cell death 1 inhibitor (PD1) liver transplantation for HCC our center analyzed the data these with purpose investigating safety feasibility preoperative PD1...
MicroRNAs (miRNAs) play a pivotal role in hepatocellular carcinoma (HCC) progression and have been confirmed to participate the carcinogenesis development of HCC. However, relationship between miR-497-5p HCC remains unclear.Kaplan-Meier curve analysis log-rank test were used investigate efficacy on overall survival (OS) disease-free (DFS) patients with According vitro experiments, programmed cell death 4 (PDCD4) was target by dual-luciferase activity assay. The PDCD4 proliferation metastasis...
Maspin, a non-inhibitory member of the serine protease inhibitor superfamily, has been characterized as tumor suppressor gene in multiple cancer types. Chemotherapeutic insensitivity is one major obstacles to effectively treating muscle invasive bladder (MIBC). This study was conducted investigate role and probable mechanism Maspin enhancing cisplatin chemosensitivity vitro MIBC patients. expression quantified by qRT-PCR two cell lines (T24 5637). After successful established overexpression...
Fatty liver disease is one of the leading causes chronic damage in western countries. Approximately 25% adults United States have fatty livers absence excessive alcohol consumption, a condition termed nonalcoholic (NAFLD). Little known about prevalence and genetic background NAFLD or factors that determine its development. In this study, we used Gene-Cloud Biotechnology Information bioinformatics platform to carry out comprehensive analysis identifying differentially expressed genes (DEGs),...
Abstract Cholesterol metabolism plays a critical role in the progression of hepatocellular carcinoma (HCC), but it is not clear how cholesterol regulated. The tubulin beta class I genes (TUBBs) are associated with prognosis many different cancers. To confirm function TUBBs HCC, Kaplan–Meier method and Cox analyses were performed using TCGA GSE14520 datasets. A higher expression TUBB2B an independent prognostic factor for shorter over survival HCC patients. Deletion hepatocytes inhibits...
Background. Ischemia-reperfusion injury (IRI) has been considered an inevitable event in organ transplantation since the first successful kidney transplant was performed 1954. To avoid IRI, we have established a novel procedure called ischemia-free transplantation. Here, describe case of (IFKT). Meterials and methods. The graft donated by 19-year-old brain dead donor. recipient 47-year-old man with end-stage diabetic nephropathy. procured, preserved implanted without cessation blood supply...
Background/Aims: The shortage of donor liver hinders the development transplantation. This study aimed to clarify poor outcomes functionally marginal grafts (FMLs) and provide evidence for improvement ischemia-free transplantation (IFLT) after FML transplantation.Methods: Propensity score matching was used control confounding factors. group were compared demonstrate negative impact FMLs on patients. We clinical improvements different surgical types. To elucidate underlying mechanism, we...
The abnormal expression of long noncoding RNAs (lncRNAs) is associated with human carcinoma. present study aimed to investigate the mechanisms underlying function lncRNA AK002107 in progression hepatocellular carcinoma (HCC). differential lncRNAs between HCC and paired nontumor tissues was identified using microarrays, correlation clinical prognosis analyzed. We investigated role tumor biology vitro 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), colony formation,...
Aim: To explore molecular mechanisms underlying liver ischemia-reperfusion injury (IRI). Materials & methods: Four Gene Expression Omnibus datasets comprising transplantation data were collected for a comprehensive analysis. A proteomic analysis was performed and used correlations with transcriptomic. Results conclusion: Ten differentially expressed genes co-upregulated in four datasets, including ATF3, CCL4, DNAJB1, DUSP5, JUND, KLF6, NFKBIA, PLAUR, PPP1R15A TNFAIP3. The combined...
Transplant rejection remains a major barrier to graft survival and involves diversity of cell types. However, the heterogeneity each type in allograft poorly defined. In present study, we used single-cell RNA sequencing technology analyze graft-infiltrating cells describe types states associated with acute mouse heart transplant model. Unsupervised clustering analysis revealed 21 distinct populations. Macrophages formed five clusters: two resident macrophage groups, infiltrating groups one...
Background. Ischemia-free liver transplantation (IFLT) has been innovated to avoid graft ischemia during organ procurement, preservation, and implantation. However, the metabolism activity of donor livers between in situ ex normothermic machine perfusion (NMP) conditions, standard criteria extend remains unknown. Methods. During IFLT, plasma samples were collected both at portal vein hepatic procurement NMP. An ultra-high performance liquid chromatography-mass spectrometry was conducted...
It has been shown that normothermic machine perfusion (NMP), a novel preservation method, is able to assess and resuscitate liver grafts with risk factors. However, there no consistent criteria for the assessment of NMP. Ischemia-free transplantation (IFLT) includes innovative surgical techniques NMP, which can protect from ischemia throughout organ procurement, preservation, implantation. In our center, 28 human livers donation after brain death donors were subjected IFLT between July 2017...
Ischemia reperfusion injury (IRI) is an adverse factor for hepatocellular carcinoma (HCC) recurrence after liver transplantation. Ischemic-free transplantation (IFLT) a novel transplant procedure that can largely reduce or even prevent IRI, but the clinical relevance of IFLT and HCC are still unknown. This retrospective study compared survival outcomes, recurrence, perioperative data IRI severity following (LT). 30 patients received 196 conventional (CLT) were chosen entire cohort between...
Abstract Background The global incidence of liver diseases is rising, yet there remains a dearth precise research models to mimic these diseases. use normothermic machine perfusion (NMP) study diseased livers recovered from transplantation (LT) recipients presents promising avenue. Accordingly, we have developed system tailored specifically for the human whole and present our experience NMP livers. Methods Six LT with different diagnoses were collected. connected that circulated perfusate,...
Evaluating the safety and efficacy of implanting a liver with islet grafts into patients end-stage disease diabetes mellitus (DM).
It has been shown that combined liver-kidney normothermic machine perfusion (NMP) is able to better maintain the circuit's biochemical milieu. Nevertheless, whether superior liver alone in protecting livers from donation after circulatory death (DCD) unclear. We aimed test hypothesis and explored mechanisms. Livers 15 DCD pig donors were subjected either static cold storage (group A), liver-alone NMP B), or C). preserved for 6 hours reperfused ex vivo 2 simulate transplantation transplanted...
Afatinib is a highly selective, irreversible inhibitor of the epidermal growth factor receptor (EGFR) and human EGFR 2 (HER-2). Although preclinical clinical studies have indicated that afatinib has antitumor activity efficacy in non-small cell lung carcinoma, head neck squamous carcinoma breast cancer, there are few investigating its inhibitory effect on bladder cells. In this study, was investigated T24 cancer line. The line treated with at various concentrations (0, 1, 5, 10 20 µmol/l)....
To isolate, purify, sequence and characterize nephrocalcin (NC), a urinary protein that may be an important determinant of calcium oxalate (CaOx) kidney-stone disease.Proteins were isolated from human urine using cellulose resin columns sequenced Edman degradation SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Inhibition CaOx crystal growth by the proteins was assessed measuring deposition 14C-labelled CaOx.A assumed to NC on basis SDS-PAGE, inhibitory filtration properties healthy...