A5010293109- Pancreatic function and diabetes
- Diabetes and associated disorders
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Diet, Metabolism, and Disease
- Immune Cell Function and Interaction
- Diabetes Management and Research
- Liver Disease Diagnosis and Treatment
- T-cell and B-cell Immunology
- Genetic Associations and Epidemiology
- Adipose Tissue and Metabolism
- Neurotransmitter Receptor Influence on Behavior
- Cancer-related molecular mechanisms research
- Single-cell and spatial transcriptomics
- Diabetes Treatment and Management
- Nuclear Receptors and Signaling
- Metabolism, Diabetes, and Cancer
- GDF15 and Related Biomarkers
- Craniofacial Disorders and Treatments
- Nutrition and Health in Aging
- Receptor Mechanisms and Signaling
- Pain Mechanisms and Treatments
- Cleft Lip and Palate Research
- Renal Diseases and Glomerulopathies
- Birth, Development, and Health
Nanjing Medical University
2016-2025
Jiangsu Province Hospital
2017-2025
OBJECTIVE Type 1 diabetes (T1D) is a highly heritable disease with much lower incidence but more adult-onset cases in the Chinese population. Although genome-wide association studies (GWAS) have identified >60 T1D loci Caucasians, less known Asians. RESEARCH DESIGN AND METHODS We performed first two-stage GWAS of using 2,596 autoantibody-positive case subjects and 5,082 control Han population evaluated associations between risk age fasting C-peptide levels at diagnosis. RESULTS...
This study aims to investigate the associations between MTNR1B rs724030 A>G variant and prediabetes risk, along with their correlations clinical features, including plasma glucose serum insulin levels during oral tolerance test (OGTT), islet function, resistance, lipid levels. In particular, we investigated whether there are sex dimorphisms in impact of this on function/insulin resistance. We included 3415 glucose-tolerant healthy 1744 individuals based OGTT. Binary logistic regression was...
Islets damage is a major abnormality underling diabetes. Recent studies suggested the value of exosomes in diagnosis. This study aimed to investigate impact injury factors on miRNA profiles islet and determine whether circulating exosomal miRNAs suitable as biomarkers islets damage. were isolated from ICR mice induced vitro by mixed cytokines (Tumor Necrosis Factor-α, Interleukin -1β Interferon-γ) or streptozotocin (STZ), derived cultural supernatant. Using microarray analysis, we found 22...
Non‑alcoholic fatty liver disease (NAFLD) is a widespread threat to human health. However, the present screening methods for NAFLD are time‑consuming or invasive. The study aimed assess potential of microRNAs (miRNAs/miRs) in serum extracellular vesicles (EVs) as biomarker NAFLD. C57BL/6J mice were fed either 12‑week high‑fat diet (HFD) standard chow establish and control groups, respectively. Serum samples obtained from mouse model NAFLD, well 50 patients with healthy individuals, EVs...
Type 1 diabetes (T1D) is a T cell-mediated autoimmune disease. Increased incidence of T1D was reported in patients receiving IFN-α treatment. However, the exact mechanisms that facilitate pathogenesis are not fully understood. To explore mechanism on immune system and islets, non-obese diabetic (NOD) mice were injected with progression insulitis assessed by haematoxylin eosin (HE) staining, immunohistochemical flow cytometry analysis. Transcriptional profiling islets treated explored...
To investigate the molecular mechanisms underlying islet dysfunction and insulin resistance in diet-induced diabetes, we conducted temporal RNA sequencing of tissues responsible for secretion (islets) action (liver) every 4 weeks mice on high-fat (HFD) or chow diet 24 weeks, linking to longitudinal profile metabolic characteristics. The diverse responses α, β, δ cells glucose palmitate indicated HFD-induced dynamic deterioration function from dysregulation failure. Insulin developed with...
Abstract Signaling abnormalities play important roles during podocyte injury and have been indicated as crucial events for triggering many glomerular diseases. There is emerging evidence demonstrating significant improvements in preventing renal restoring podocytes after islet transplantation. However, whether signaling affect the therapeutic efficacy of transplantation remain unclear. This study was established to investigate impact Notch-1 activation on restoration Experiments were...
MicroRNAs (miRNAs) have been implicated in β cells dysfunction. Previous studies indicated that miR-127 was specifically abundant and one of its target genes, Kif3b, promoted cell proliferation. However, the impact miR-127-Kif3b axis on remains unknown. In this study, we revealed level declined both islets from mice with a high-fat diet MIN6 elevated glucose treatment. The attenuated proliferation by repressing Kif3b expression without affecting apoptosis cycle, it dampened insulin...
Impaired follicular regulatory T (Tfr) cells enhance helper activity, resulting in the expansion of autoreactive B and autoantibody production. However, role Tfr pathogenesis type 1 diabetes (T1D) is unclear.We evaluated expression changes function circulating by studying patients with T1D alongside those 2 (T2D), first-degree relatives patients, healthy controls. We also investigated effects on disease development nonobese diabetic (NOD) mice an adoptive transfer model.Tfr were...
Abstract Previous studies exploring the influence of glycemic variability (GV) on pathogenesis distal symmetrical polyneuropathy (DSPN) in type 1 diabetes (T1DM) produced conflicting results. The aim this study was to assess relationship between GV and DSPN T1DM. Adults with T1DM were included cross-sectional asked undergo 3-day CGM. quantified by coefficient variation (CV) mean amplitude glucose excursions (MAGE) obtained from Clinical characteristics biochemical assessments collected for...
Acetylcholine (ACh) and norepinephrine (NE) are representative neurotransmitters of parasympathetic sympathetic nerves, respectively, that antagonize each other to coregulate internal body functions. This also includes the control different kinds hormone secretion from pancreatic islets. However, molecular mechanisms have not been fully elucidated, whether innervation in islets is abnormal diabetes mellitus remains unclear.Immunofluorescence colocalization islet perfusion were performed...
Previous genomewide association studies (GWAS) identified a region near MAFB at chr20q12 associated with non-syndromic orofacial clefts (NSOC) susceptibility. However, whether other SNPs in this area could independently contribute to Chinese populations remained obscure.We selected 24 based on haplotype-tagging SNP strategy and evaluated their associations risk of large-scale two-stage case-control study 1278 cases 1295 controls. Genotyping was performed Sequenom TaqMan assay. Associations...
Aims Growth differentiation factor-15 (GDF-15) and adiponectin are adipokines that regulate metabolism. This study aimed to evaluate the roles of GDF-15, adiponectin, GDF-15/adiponectin ratio (G/A ratio) as biomarkers for detecting metabolic syndrome (MS). Materials methods cross-sectional included 676 participants aged 20–70 years in Jurong, China. The were divided into four groups based on sex age (<40 ≥40 years). MS was defined according modified National Cholesterol Education...
Objective This study aims to reveal the association between JAZF1 rs864745 A>G variant and type 2 diabetes (T2D), 1 (T1D) risk, their correlation with clinical features, including islet function, autoimmunity, plasma lipid levels. Methods We included 2505 healthy controls based on oral glucose tolerance test (OGTT), 1736 unrelated T2D, 1003 autoantibody-positive T1D individuals. Binary logistic regression was performed evaluate relationships in T1D, islet-specific autoantibody status...
This study aims to examine the alterations and clinical significance of CD8+ regulatory T cell subsets in peripheral blood individuals with type 1 diabetes mellitus (T1DM). From January 2020 December 2023, a was conducted involving 40 T1DM, who visited Department Endocrinology at First Affiliated Hospital Nanjing Medical University (T1DM group). For comparison, healthy underwent routine physical examinations same hospital during this period were selected as control group. Peripheral...
Islets damage is a major abnormality underling diabetes, thus timely monitoring islets destruction critically needed. Recent studies discovered the biological functions of exosomes and suggested value in disease diagnosis prognosis. This study aimed to investigate impact injury factors on miRNA profiles islet determine whether circulating exosomal miRNAs would be suitable as biomarkers cell damage. were isolated from ICR mice induced vitro by mixed cytokines (TNFα, IL-1β IFNγ) streptozotocin...