A5023300293- Neuroinflammation and Neurodegeneration Mechanisms
- Ion Channels and Receptors
- Alzheimer's disease research and treatments
- Signaling Pathways in Disease
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Colorectal Cancer Treatments and Studies
- Ion channel regulation and function
- Cancer Treatment and Pharmacology
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Nanoparticle-Based Drug Delivery
- Nanoparticles: synthesis and applications
- Bioinformatics and Genomic Networks
- Cancer, Hypoxia, and Metabolism
- Bone Tissue Engineering Materials
- Axon Guidance and Neuronal Signaling
- Medicinal Plants and Neuroprotection
- Cutaneous Melanoma Detection and Management
- Melanoma and MAPK Pathways
- Gene expression and cancer classification
- Molecular Biology Techniques and Applications
- Anesthesia and Neurotoxicity Research
- Wnt/β-catenin signaling in development and cancer
- RNA and protein synthesis mechanisms
University of Turin
2011-2025
Accademia Albertina delle Belle Arti
2014-2024
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2017-2022
Abstract The pathogenesis of Alzheimer’s disease (AD), a slowly-developing age-related neurodegenerative disorder, is result the action multiple factors including deregulation Ca 2+ homeostasis, mitochondrial dysfunction, and dysproteostasis. Interaction these in astrocytes, principal homeostatic cells central nervous system, still poorly understood. Here we report that immortalized hippocampal astrocytes from 3xTg-AD mice (3Tg-iAstro cells) bioenergetics impaired, reduced glycolysis oxygen...
Peripheral neurotoxicity is one of the most distressing side effects oxaliplatin therapy for cancer. Indeed, patients that received experience acute and/or chronic severe sensory peripheral neuropathy. However, despite similar co-morbidities, cancer stage, demographics and treatment schedule, develop oxaliplatin-induced with remarkably different severity. This suggests individual genetic variability, which might be used to glean mechanistic insights into neurotoxicity. We characterized...
An amendment to this paper has been published and can be accessed via a link at the top of paper.
Structuring data analysis projects, that is, defining the layout of files and folders needed to analyze using existing tools novel code, largely follows personal preferences. Open Science calls for more accessible, transparent understandable research. We believe principles can be applied way projects are structured. examine structure several project templates by analyzing template repositories present in GitHub. Through visualization resulting consensus structure, we draw observations...
Endothelial colony forming cells (ECFCs) represent a population of truly endothelial precursors that promote the angiogenic switch in solid tumors, such as breast cancer (BC). The intracellular Ca2+ toolkit, which drives pro-angiogenic response to VEGF, is remodelled tumor-associated ECFCs they are seemingly insensitive this growth factor. This feature could underlie relative failure anti-VEGF therapies patients. Herein, we investigated whether and how VEGF uses signalling control...
Abstract Evidence is rapidly growing regarding a role of astroglial cells in the pathogenesis Alzheimer’s disease (AD), and hippocampus one important brain regions affected AD. While primary cultures, both from wild-type mice rodent models AD, have been useful for studying astrocyte-specific alterations, limited cell number short culture lifetime use hippocampal astrocytes. To overcome these limitations, we now established immortalized lines 3xTg-AD control (3Tg-iAstro WT-iAstro,...
The last decades have seen an explosive increase in the development of nanoparticles and their use consumer, industrial medical applications. Their fast diffusion has also raised widespread concern about potential toxic effects on living organisms, including humans: at nanoscale, they can interact with subcellular components such as membranes, proteins, lipids, nucleic acids, thus inducing unpredicted functional perturbations cells tissues. nervous tissue is a particular sensitive target,...
Astrocytes perform important housekeeping functions in the nervous system including maintenance of adequate neuronal excitability, although regulatory mechanisms are currently poorly understood. The astrocytic Ca
ABSTRACT STIM and ORAI proteins play a fundamental role in calcium signaling, allowing for influx through the plasma membrane upon depletion of intracellular stores, process known as store-operated Ca2+ entry. Point mutations that lead to gain-of-function activity either STIM1 or ORAI1 are responsible cluster ultra-rare syndromes characterized by motor disturbances platelet dysfunction. The prevalence these disorders is at present unknown. In this study, we describe generation...
Mesenchymal stem cells (MSCs) can be harvested from different sites in the oral cavity, representing a reservoir of useful for regenerative purposes. As direct comparisons between at least two types MSCs deriving same patient are surprisingly rare scientific literature, we isolated and investigated osteoinductive potential dental pulp (DPSCs) buccal fat pad (BFPSCs). were third molar pads 12 patients. The number viable was quantified through manual count. Proliferation osteodifferentiation...
Emerging evidence indicates that the TRPM8 channel plays an important role in prostate cancer (PCa) progression, by impairing motility of these cells. Here, we reveal a novel facet PCa control via direct protein-protein interaction (PPI) with small GTPase Rap1A. The functional two proteins was assessed active Rap1 pull-down assays and live-cell imaging experiments. Molecular modeling analysis allowed identification four putative residues involved TRPM8-Rap1A interaction. Point mutations...
Pancreatic Ductal Adenocarcinoma (PDAC) is characterized by an acidic microenvironment, which contributes to therapeutic failure. So far there a lack of knowledge with respect the role microenvironment in invasive process. This work aimed study phenotypic and genetic response PDAC cells stress along different stages selection. To this end, we subjected short- long-term pressure recovery pHe 7.4. treatment at mimicking edges consequent cancer cell escape from tumor. The impact acidosis was...
Store-operated Ca2+-entry is a cellular mechanism that governs the replenishment of intracellular stores Ca2+ upon depletion caused by opening Ca2+-channels. Gain-of-function mutations 2 key proteins store-operated Ca2+-entry, STIM1 and ORAI1, are associated with several ultra-rare diseases clustered as tubular aggregate myopathies. Our group has previously demonstrated mouse model bearing p.I115F mutation recapitulates main features gain-of-function disorders: muscle weakness...
The mechanical and biological behaviors of PMMA/Al2O3 composites incorporating 30 wt.%, 40 50 wt.% Al2O3 were thoroughly characterized as regards to their possible application in implant-supported prostheses. particles accounted for an increase the flexural modulus PMMA. highest value was recorded composite containing (4.50 GPa), which about 18% higher than that its unfilled counterpart (3.86 GPa). caused a decrease strength composites, due presence filler aggregates voids, though it still...
Background: It is well known that alterations in astrocytes occur Alzheimer’s disease and reactive astrogliosis one of the hallmarks disease. Recently, data has emerged suggests may also early pathogenesis Objective: The aim present work was to characterize transcriptional occurring cultured from 3xTg-AD mouse pups compared control non-transgenic mice. Furthermore, we these changes those reported by others symptomatic AD Method: We conducted a whole-genome microarray study on primary...
Pancreatic ductal adenocarcinoma (PDAC) is a deadly disease with high mortality due to early metastatic dissemination and chemoresistance. All these factors are favored by its extracellular matrix (ECM)-rich microenvironment, which also highly hypoxic acidic. Gemcitabine (GEM) still the first-line therapy in PDAC. However, it quickly deaminated inactive metabolite. Several GEM prodrugs have emerged improve cytotoxicity. Here, we analyzed how acidic/hypoxic tumor microenvironment (TME)...