A5025483224- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Fungal Biology and Applications
- Bryophyte Studies and Records
- Microbial Natural Products and Biosynthesis
- Photosynthetic Processes and Mechanisms
- Lichen and fungal ecology
- Plant Molecular Biology Research
- Natural product bioactivities and synthesis
- Antifungal resistance and susceptibility
- Wheat and Barley Genetics and Pathology
- Biocrusts and Microbial Ecology
- Plant tissue culture and regeneration
- Light effects on plants
- Botany and Plant Ecology Studies
- Biochemical and biochemical processes
- Plant-Microbe Interactions and Immunity
- Essential Oils and Antimicrobial Activity
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Plant Parasitism and Resistance
- Cell death mechanisms and regulation
- Potato Plant Research
- Plant Pathogens and Resistance
- Plant and animal studies
Shandong University
2016-2025
Feldan (Canada)
2024
Brookhaven National Laboratory
2013
Shanghai Institutes for Biological Sciences
2007
Chinese Academy of Sciences
2007
Center for Excellence in Molecular Plant Sciences
2007
Zero to Three
2006
Abstract Among plant secondary metabolites terpenoids are a structurally most diverse group; they function as phytoalex‐ins in direct defense, or signals indirect defense responses which involves herbivores and their natural enemies. In recent years, more attention has been paid to the investigation of ecological role terpenoids. The biosynthesis pathways monoterpenes, sesquiterpenes, diterpenes include synthesis C5 precursor isopentenyl diphosphate (IPP) its allylic isomer dimethylallyl...
Flavonoids ubiquitously distribute to the terrestrial plants and chalcone isomerase (CHI)-catalyzed intramolecular stereospecific cyclization of chalcones is a committed step in production flavonoids. However, so far bona fide CHIs are found only vascular plants, their origin evolution remains elusive. We conducted transcriptomic and/or genomic sequence search, subsequent phylogenetic analysis, detailed biochemical genetic characterization explore potential existence CHI proteins basal...
During the course of evolution land plants, different classes flavonoids, including flavonols and anthocyanins, sequentially emerged, facilitating adaptation to harsh terrestrial environment. Flavanone 3β-hydroxylase (F3H), an enzyme functioning in flavonol anthocyanin biosynthesis a member 2-oxoglutarate-dependent dioxygenase (2-ODD) family, catalyzes hydroxylation (2S)-flavanones dihydroflavonols, but its origin remain elusive. Here, we demonstrate that functional flavone synthase Is (FNS...
Phenylpropanoids play important roles in plant physiology and the enzyme 4-coumarate: coenzyme A ligase (4CL) catalyzes formation of thioesters. Despite extensive characterization various plants, functions 4CLs liverwort Marchantia paleacea remain unknown. Here, four from M. were isolated functionally analyzed. Heterologous expression Escherichia coli indicated presence different enzymatic activities enzymes. Mp4CL1 Mp4CL2 able to convert caffeic, p-coumaric, cinnamic, ferulic,...
The distribution of type I and II chalcone isomerases (CHIs) in plants is highly family specific. We have previously reported that ancient land plants, such as the liverworts Selaginella moellendorffii, harbor CHIs. To better understand function evolution CHI-fold proteins, transcriptomic data obtained from 52 pteridophyte species were subjected to sequence alignment phylogenetic analysis. residues determining I/II CHI identity CHIs identical those enzymatic characterization a sample 24...
The vitro antifungal activity of retigeric acid B (RAB), a pentacyclic triterpenoid from the lichen species Lobaria kurokawae, was evaluated alone and in combination with fluconazole, ketoconazole, itraconazole against Candida albicans using checkerboard microdilution time-killing tests. MICs for RAB 10 different C. isolates ranged 8 to 16 microg/ml. A synergistic action azole observed azole-resistant strains, whereas or indifferent effects were azole-sensitive strains when interpreted by...
Candida albicans, the most prevalent fungal pathogen, undergoes yeast-to-hyphal switch which has long been identified as a key virulence factor. We showed here that lichen-derived small molecule retigeric acid B (RAB) acted an inhibitor significantly inhibited filamentation of C. leading to prolonged survival nematodes infected by albicans. Quantitative real-time PCR analysis and intracellular cAMP measurement revealed RAB regulated Ras1-cAMP-Efg1 pathway reducing level inhibit hyphae...
FNS I is a 2‐oxoglutarate dependent dioxygenase (2‐ODD) found mainly in species of the Apiaceae family. Here, an cDNA sequence was isolated from liverwort Plagiochasma appendiculatum (Aytoniaceae) and characterized. The recombinant protein exhibited high activity catalyzing conversion naringenin to apigenin 2‐hydroxynaringenin. critical residue for flavanone‐2‐hydroxylation Tyr240, as identified homology modeling site‐directed mutagenesis. also showed some flavonol synthase activity, it can...
Abstract Background The basic helix-loop-helix (bHLH) transcription factors (TFs), as one of the largest families TFs, play important roles in regulation many secondary metabolites including flavonoids. Their involvement flavonoids synthesis is well established vascular plants, but not yet bryophytes. In liverworts, both bisbibenzyls and are derived through phenylpropanoids pathway share several upstream enzymes. Results this study, we cloned characterized function PabHLH1, a bHLH family...
Isoflavones are rich natural compounds present in legumes and essential for plant growth development. Moreover, they beneficial animals humans. primarily found as glycoconjugates, including calycosin-7-O-β-d-glucoside (CG) Astragalus membranaceus, a legume. However, the glycosylation mechanism of isoflavones A. membranaceus remains unclear. In study, three uridine diphosphate (UDP)-glycosyltransferases (UGTs) that may be involved biosynthesis isoflavone were identified transcriptome...
Biofilm formation plays a key role in the life cycles and subsistence of many microorganisms. The human fungal pathogen Candida albicans has high propensity to develop biofilms resulted resistant traditional antifungal agents. Biofilms are composed mixture cell types, including yeast, pseudohyphal hyphal cells, hyphae prominent feature biofilms. Riccardin D is macrocyclic bisbibenzyl isolated from liverwort Dumortiera hirsute our laboratory. In present investigation, XTT...