A5089397604- Liver Disease Diagnosis and Treatment
- Drug Transport and Resistance Mechanisms
- Drug-Induced Hepatotoxicity and Protection
- Pharmacogenetics and Drug Metabolism
- Analytical Chemistry and Chromatography
- Genomics, phytochemicals, and oxidative stress
- Mass Spectrometry Techniques and Applications
- Plant-derived Lignans Synthesis and Bioactivity
- Microfluidic and Capillary Electrophoresis Applications
- Liver physiology and pathology
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Alcohol Consumption and Health Effects
- Cancer, Hypoxia, and Metabolism
- Graphene and Nanomaterials Applications
- Parasitic infections in humans and animals
- Inflammatory Bowel Disease
- Diabetes and associated disorders
- Inflammatory mediators and NSAID effects
- Gout, Hyperuricemia, Uric Acid
- Glutathione Transferases and Polymorphisms
- Papaya Research and Applications
- Protein Degradation and Inhibitors
- Biological Activity of Diterpenoids and Biflavonoids
- Carbon and Quantum Dots Applications
Sun Yat-sen University
2011-2024
Wuhan University of Technology
2022-2024
State Key Laboratory of Pollution Control and Resource Reuse
2023-2024
Nanjing University
2023-2024
Xihua University
2024
Zhejiang University
2019-2023
First Affiliated Hospital Zhejiang University
2019-2023
Ghent University
2022
Maternal and Child Health Care Hospital of Bao'an
2016
The First Affiliated Hospital, Sun Yat-sen University
2014-2015
Allyl isothiocyanate (AITC), a classic anti-inflammatory and antitumorigenic agent, was recently identified as potential treatment for obesity insulin resistance. However, little is known about its direct impact on the liver.To investigate effect underlying mechanism of AITC in nonalcoholic fatty liver disease (commonly referred to NAFLD).To establish mouse cellular model NAFLD, C57BL/6 mice were fed high fat diet (HFD) 8 wk, AML-12 cells treated with 200 μM palmitate acid 24 h. For...
The simultaneous dosing of numerous compounds followed by multiple-component analysis using LC−MS−MS (the N-in-1 approach) has significantly improved the throughput drug-screening process. However, plasma samples still need to be extracted before analysis, which frequently limits assay. In this work, a high-throughput on-line extraction technique been developed for assays high-flow column-switching technique. assays, high sensitivity is required since dose level generally reduced minimize...
Epigenetic alterations, such as histone acetylation, regulate the signaling outcomes and phenotypic responses of fibroblasts after growth factor stimulation. The bromodomain extra-terminal domain–containing proteins (Brd) bind to acetylated residues, resulting in recruitment components transcriptional machinery subsequent gene transcription. Given central importance tissue fibrosis, this study sought determine role Brd human lung (LFs) stimulation murine bleomycin model fibrosis. Using small...
Oleanolic acid (OA) is a natural triterpenoid and has been demonstrated to protect against varieties of hepatotoxicants. Recently, however, OA at high doses was reported produce apparent cholestasis in mice. In this study, we characterized the protective effect low lithocholic (LCA)–induced mice explored further mechanisms. cotreatment (5, 10, 20 mg/kg, i.p.) significantly improved mouse survival rate, attenuated liver necrosis, decreased serum alanine aminotransferase, aspartate alkaline...
Acetaminophen (APAP) overdose is the most frequent cause of drug-induced acute liver failure. Schisandra sphenanthera a traditional hepato-protective Chinese medicine and Schisandrol B (SolB) one its major active constituents. In this study, protective effect SolB against APAP-induced hepatotoxicity in mice involved mechanisms were investigated. Morphological biochemical assessments clearly demonstrated injury. pretreatment significantly attenuated increases alanine aminotransferase...
Background and Purpose Currently, ursodeoxycholic acid obeticholic are the only two FDA‐approved drugs for cholestatic liver diseases. Thus, new therapeutic approaches need to be developed. Here we have evaluated anti‐cholestasis effects of Schisandrol B (SolB), a bioactive compound isolated from Schisandra sphenanthera . Experimental Approach Hepatoprotective effect SolB against intrahepatic cholestasis, induced by lithocholic (LCA), was in mice. Metabolomic analysis gene were used assess...
Epidemiological studies have suggested a link between vitamin D deficiency and increased risk for nonalcoholic fatty liver disease (NAFLD); however, the underlying mechanisms remained unclear. Here, using both clinical samples experimental rodent models along with several biochemical approaches, we explored specific effects of in NAFLD pathology. Serum levels were significantly lower individuals high-fat diet (HFD)-fed mice than healthy controls chow-fed mice, respectively. Vitamin...
The melanocortin 4 receptor (MC4R) plays an important role in body weight regulation and energy homeostasis. Administration of peptidic MC4R antagonists (usually by intracerebro ventricular injection) has been shown the literature to increase and/or food intake several rodent models. We report here identification a novel nonpeptidic antagonist its effects on tumor-induced loss mice following peripheral administration.
<i>Schisandra sphenanthera</i> is widely used as a tonic and restorative in many countries to enhance the function of liver other organs. Wuzhi tablet (WZ) preparation an ethanol extract sphenanthera</i>. Our previous study demonstrated that WZ exerted protective effect toward acetaminophen (APAP)-induced hepatotoxicity. However, molecular mechanisms this protection remain unclear. This aimed determine what pathways contributed hepatoprotective effects against APAP toxicity. Administration 3...
Background/Aims: To explore whether Nrf2 was associated with drug-resistance in cisplatin resistant A549 (A549/DDP) cells, and if cryptotanshinone (CTS), one of the bioactive compounds isolated from roots Salvia miltiorrhiza Bunge (Danshen), could enhance sensitivity A549/DDP cells towards cisplatin. Methods: were subjected to various treatments, then Sulforhodamine B (SRB) assay, flow cytometry analysis western immunoblotting applied determine IC50, apoptotic status expressions its...
We previously reported that the ethanol extract of <i>Schisandra sphenanthera</i> [Wuzhi (WZ) tablet] significantly protects against acetaminophen-induced hepatoxicity. However, whether WZ exerts a protective effect cholestasis remains unclear. In this study, on lithocholic acid (LCA)–induced intrahepatic in mice was characterized and involved mechanisms were investigated. pretreatment (350 mg/kg) with LCA reversed liver necrosis decreased serum alanine aminotransferase, aspartate alkaline...
Carnitine palmitoyltransferase 1C (CPT1C), an enzyme located in the outer mitochondria membrane, has a crucial role fatty acid transport and oxidation. It is also involved cell proliferation potential driver for cancer senescence. However, its upstream regulatory mechanism unknown. Peroxisome proliferator activated receptor α (PPARα) ligand-activated transcription factor that regulates lipid metabolism tumor progression. The current study aimed to elucidate whether how PPARα CPT1C then...
Aberrant expression of Nicotinamide N-methyltransferase (NNMT) has been reported in pancreatic cancer. However, the role NNMT cancer development remains elusive. Therefore, present study was to investigate impact on cell proliferation, metastatic potential and survival under metabolic stress.Pancreatic line PANC-1 transfected with plasmid or small interfering RNA overexpress knockdown intracellular expression, respectively. Rate proliferation monitored. Transwell migration matrigel invasion...
It is well known that nonalcoholic fatty liver disease (NAFLD) associated with insulin resistance (IR). LB100, a serine/threonine protein phosphatase 2A (PP2A) inhibitor, closely related to IR. However, there little data regarding its direct influence on NAFLD.To elucidate the effect and underlying mechanism of LB100 in NAFLD.After 10 wk high fat diet (HFD) feeding, male C57BL/6 mice were injected intraperitoneally vehicle or for an additional 6 (three times week). The L02 cell line was...
We previously showed that the vitamin D receptor (VDR) plays a crucial role in acute inflammatory bowel disease and intestinal fibrosis is common complication of Crohn's (CD). Epithelial–mesenchymal transition (EMT) an important hallmark fibrogenesis through which epithelial cells lose their phenotype transform into mesenchymal cells. It known VDR essential integrity mitochondrial function, but its remains unknown. Here, we investigated whether involved dysfunction results EMT fibrosis....
With the ever-increasing workload from a variety of in vitro and vivo screening procedures, new analytical methodologies to perform bioanalysis an accurate high-throughput manner are great demand. In this work, monolithic columns were used instead conventional particulate HPLC chromatographic separations. Because pressure drop on column was considerably lower than that column, high flow rate (6 mL/min) for 4.6 x 50 mm with total backpressure about 61 bar measured using acetonitrile/water...
Single-dose pharmacokinetics of 1-aminobenzotriazole (ABT), a potent nonspecific inhibitor cytochromes P450 (P450s), were characterized after oral administration to mice and guinea pigs at doses 50, 100, 150 mg/kg using serial sampling in both species. Only 30-μl blood samples drawn from jugular vein-cannulated Microvette capillary tubes containing lithium heparin. A comparison the antipyrine (AP) administered i.v. 20 followed by terminal techniques yielded similar results. The ABT...
Acetaminophen (APAP) hepatotoxicity is the most common cause of drug-induced liver injury and <i>N</i>-acetylcysteine (NAC) primary antidote APAP poisoning. Wuzhi tablet (WZ), active constituents well identified quantified, a preparation an ethanol extract <i>Schisandra sphenanthera</i> exerts protective effect toward APAP-induced in mice. However, clinical use WZ to rescue acute mechanisms involved therapeutic remain unclear. Therefore, on was compared with NAC mice, molecular pathways...