A5000222532- Vector-borne infectious diseases
- Viral Infections and Vectors
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Bird parasitology and diseases
- Mosquito-borne diseases and control
- Study of Mite Species
- Insect and Pesticide Research
- Long-Term Effects of COVID-19
- Vector-Borne Animal Diseases
- Computational Drug Discovery Methods
- Molecular Biology Techniques and Applications
- Bacillus and Francisella bacterial research
- Insect symbiosis and bacterial influences
- Virology and Viral Diseases
- Malaria Research and Control
- Viral Infections and Outbreaks Research
- Rabies epidemiology and control
Hokkaido University
2021-2024
In parallel with vaccination, oral antiviral agents are highly anticipated to act as countermeasures for the treatment of coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome 2 (SARS-CoV-2). Oral medication demands not only high activity but also target specificity, favorable bioavailability, and metabolic stability. Although a large number compounds have been identified potential inhibitors SARS-CoV-2 infection in vitro, few proven be effective vivo....
Viral infections produce double-stranded RNA (dsRNA) during replication, which trigger host innate immune responses. Immunoassays using anti-dsRNA antibodies have been widely employed to detect viral dsRNA. In this study, we used a luciferase-based dsRNA biosensor consists of protein kinase R (PKR)-derived binding domains fused split luciferase subunits. Here, demonstrate the use measure in specimens extracted from cells infected with Japanese encephalitis virus (JEV). Moreover, reacts...
Pulmonary infection with SARS-CoV-2 stimulates host immune responses and can also result in the progression of dysregulated critical inflammation. Throughout pandemic, management treatment COVID-19 has been continuously updated a range antiviral drugs immunomodulators. Monotherapy oral antivirals proven to be effective COVID-19. However, should initiated early stages ensure beneficial therapeutic outcomes, there is still room for further consideration on strategies using antivirals.
Emerging and reemerging tick-borne virus infections caused by orthonairoviruses (family Nairoviridae ), which are genetically distinct from Crimean-Congo hemorrhagic fever virus, have been recently reported in East Asia. Here, we established a mouse infection model using type-I/II interferon receptor-knockout mice (AG129 mice) both for better understanding of the pathogenesis these validation antiviral agents Yezo (YEZV), novel orthonairovirus causing febrile illnesses associated with tick...
Abstract In parallel with vaccination, oral antiviral agents are highly anticipated to act as countermeasures for the treatment of coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Oral medication demands not only high activity but also target specificity, favorable bioavailability, and metabolic stability. Although a large number compounds have been identified potential inhibitors SARS-CoV-2 infection in vitro , few proven...
Poultry red mites (PRMs, Dermanyssus gallinae) are harmful ectoparasites that affect farmed chickens and cause serious economic losses in the poultry industry worldwide. Acaricides used for PRM control; however, some PRMs have developed acaricide-resistant properties, which indicated need different approaches control. Therefore, it is necessary to elucidate biological status of develop alternative control strategies. Quantitative polymerase chain reaction (qPCR) allows analysis at transcript...
Poultry red mite (PRM; Dermanyssus gallinae) is a hazardous, blood-sucking ectoparasite of birds that constitutes threat to poultry farming worldwide. Acaricides, commonly used in farms prevent PRMs, are not effective because the rapid emergence acaricide-resistant PRMs. However, vaccination may be promising strategy control PRM. We identified novel cystatin-like molecule PRMs: Dg-Cys. Dg-Cys mRNA expression was detected midgut and ovaries, all stages life. The PRM nymphs were artificially...
Poultry red mites (PRMs, Dermanyssus gallinae) are hematophagous ectoparasites that negatively affect egg production, which causes serious economic losses to the poultry industry worldwide. Currently, emergence of acaricide-resistant PRMs has impeded PRM control in farms. Several alternatives for acaricide use have been described managing PRM-caused problems. Vaccination is among methods controlling houses. several candidates vaccine antigens identified. This study identified a cysteine...
Abstract The most conserved fusion loop (FL) domain present in the flavivirus envelope protein has been reported as a dominant epitope for cross-reactive antibodies to mosquito-borne flaviviruses (MBFVs). As result, establishing accurate serodiagnosis MBFV infections difficult anti-FL are induced by both natural infection and following vaccination. In this study, we modified FL overcome cross-reactivity. We showed that of lineage I insect-specific (ISFV) differences antigenicity from those...
Poultry red mites (Dermanyssus gallinae, PRM) are dangerous ectoparasites that infest chickens and threaten the poultry industry worldwide. PRMs usually develop resistance to chemical acaricides, necessitating development of more effective preventive agents, vaccination could be an alternative strategy for controlling PRMs. The suitability plasma membrane proteins expressed in midguts as vaccine antigens was evaluated because these molecules exposed antibodies ingested blood binding...
Rabies virus (RABV) is the causative agent of rabies, a lethal neurological disease in mammals. RABV strains can be classified into fixed (laboratory strains) and street (field/clinical strains), which have different properties including cell tropism neuroinvasiveness. Toyohashi strain isolated Japan from an imported case had been bitten by rabid dog Philippines. In order to facilitate molecular studies RABV, we established reverse genetics (RG) system for study strain. The recombinant was...
West Nile virus (WNV) is a mosquito-borne flavivirus that causes encephalitis in humans and infects crocodiles, resulting rashes neurological signs. In Zambia, two distinct lineages of WNV have been detected neighbouring areas: lineage 2 mosquitoes 1a farmed crocodiles. Considering the risk direct or vector-mediated transmission from crocodiles to mammals, it necessary elucidate pathogenicity strains derived this study, was successfully isolated naturally infected (Croc110/2019/1/ZM,...
Migratory birds carry ticks harboring various pathogens, including the zoonotic Yezo virus. In Hokkaido, Japan, we collected from migratory during 2020-2021. Eight of 385 pools, comprising 2,534 ticks, tested positive for virus RNA, suggesting might be spread through flyways birds.
Abstract Pulmonary infection with SARS-CoV-2 stimulates host immune responses and can also result in the progression of dysregulated critical inflammation. Throughout pandemic, management treatment COVID-19 has been continuously updated a range antiviral drugs immunomodulators. Monotherapy oral antivirals proven to be effective COVID-19. However, should initiated early stages ensure beneficial therapeutic outcomes, there is still room for further consideration on strategies using antivirals....