A5062577479- Liver physiology and pathology
- Gastric Cancer Management and Outcomes
- Inflammatory mediators and NSAID effects
- NF-κB Signaling Pathways
- Cell death mechanisms and regulation
- Esophageal Cancer Research and Treatment
- Organ Transplantation Techniques and Outcomes
- Helicobacter pylori-related gastroenterology studies
- Cell Adhesion Molecules Research
- Gastrointestinal Tumor Research and Treatment
- Cancer Immunotherapy and Biomarkers
- Glycosylation and Glycoproteins Research
- Immune Response and Inflammation
- Estrogen and related hormone effects
- Cancer, Hypoxia, and Metabolism
- Metastasis and carcinoma case studies
- Oral and Maxillofacial Pathology
- Esophageal and GI Pathology
- Virus-based gene therapy research
- Immune Cell Function and Interaction
- Cancer-related Molecular Pathways
- Phagocytosis and Immune Regulation
- Renin-Angiotensin System Studies
- Erosion and Abrasive Machining
- Cancer Mechanisms and Therapy
Osaka University
2002-2024
Shimane University
2024
Center for Global Health
2014-2024
National Center for Global Health and Medicine
2014-2024
Tokyo Dental College
2021-2024
Minoh City Hospital
2023-2024
Tokushima University
1996-2023
Osaka Medical Center for Cancer and Cardiovascular Diseases
1996-2020
Suita Municipal Hospital
2020
Osaka National Hospital
2016
Background Plasma uric acid has been associated with hypertension in a variety of disorders, and shown to be predictive hypertension. The mechanistic role the development is not known however. Method We tested hypothesis that stimulates vascular smooth muscle cell (VSMC) proliferation oxidative stress by stimulating renin–angiotensin system (RAS). Rat VSMC were exposed 0–300 μmol for 48 h. Results Uric (200 300 μmol) stimulated as measured thymidine uptake. This effect was prevented 10−6 mol...
Injury of endothelial cells has been assumed to be an initial trigger the development atherosclerosis. In this study, we investigated molecular mechanisms cell death induced by hypoxia, which leads oxidative stress. To study relation between hypoxia-induced and activation nuclear factor-κB (NF-κB) in a hypoxic state, evaluated effect 2 antioxidant drugs, probucol pyrrolidine dithiocarbamate (PDTC), on human apoptosis. Although treatment aortic resulted significant decrease number increase...
Objective In both rheumatoid arthritis and collagen-induced (CIA), the nuclear factor κB (NF-κB) transcription plays a pivotal role in coordinated transactivation of many cytokines related to pathogenesis. This study investigated whether synthetic double-stranded DNA that show high affinity for NF-κB could be introduced vivo as “decoy” cis elements bind block activation such proinflammatory cytokine genes interleukin-1 (IL-1) tumor necrosis α (TNFα), thus suppress severity joint destruction....
Abstract —The transcription factor nuclear factor-κB (NF-κB) plays a pivotal role in the coordinated transactivation of cytokine and adhesion molecule genes involved endothelial activation. Although recent reports have documented contribution NF-κB to apoptosis, it is still controversial. Especially, apoptosis largely unknown. Hypoxia significantly induced human aortic cell death time-dependent manner ( P <0.01), accompanied by Decrease total number increase apoptotic cells hypoxia were...
Previous studies showed that the epidermal growth factor receptor (EGFR) can be transactivated by platelet-derived (PDGF) stimulation and EGFR transactivation is required for PDGF-stimulated cell migration. To investigate mechanism cross talk between PDGF β (PDGFβR) EGFR, we stimulated rat aortic vascular smooth muscle cells (VSMC) with 20 ng of PDGF/ml. Transactivation defined tyrosine phosphorylation, occurred same time course as PDGFβR activation. Basal formation PDGFβR-EGFR heterodimers...
Hepatocyte growth factor (HGF), a member of the angiogenic factors, may play pivotal role in regulation endothelial cells, inasmuch as HGF shows mitogenic and antiapoptotic actions cells. Because mechanism these is still unclear, we examined signal transduction system human aortic Treatment cells with recombinant (rHGF) resulted significant increase DNA synthesis assessed by thymidine incorporation. Importantly, phosphorylation extracellular signal–related kinase (ERK) Akt rHGF was clearly...
Although hepatocyte growth factor (HGF), a novel angiogenic factor, plays an important role in angiogenesis, regulation of local HGF production under hypoxia has not yet been clarified vascular smooth muscle cells (VSMC) and endothelial (EC). Thus, we have studied the hypoxia-induced injury expression response to hypoxia.
The association between the tumor microenvironment (TME) and treatment response or survival has been a recent focus in several types of cancer. However, most study materials are resected specimens that were completely modified by prior chemotherapy; therefore, unmodified host immune condition not yet clarified. aim present was to evaluate relationship TME assessed pre-therapeutic biopsy samples chemoresistance esophageal cancer (EC). A total 86 endoscopic from EC patients who received...
Abstract. Glomerular expression of cytokines, interleukin-1 (IL-1), and tumor necrosis factor-α (TNF-α), together with leukocytic infiltration, are prominent features in crescentic glomerulonephritis. Because these cytokines targets for nuclear transcription factor-κB (NF-κB), the use NF-κB decoy oligodeoxynucleotide (ODN) treatment was evaluated an experimental disease model. Crescentic glomerulonephritis induced primed Wistar rats by injection sheep antiglomerular basement membrane serum....
Abstract —Although cAMP is an important second messenger that plays a pivotal role in the regulation of platelet aggregation and dilatation blood vessels, little known about action on growth vascular smooth muscle cells (VSMCs). Thus, we initially studied effects accumulation by using various stimulants, including phosphodiesterase type 3 inhibitor (cilostazol) human aortic VSMC growth. Accumulation inhibited platelet-derived factor (PDGF)-stimulated dose-dependent manner ( P <0.01),...
Abstract Background No reliable marker has been identified to predict postoperative recurrence of gastric cancer. We designed a clinical trial investigate the utility serum NY-ESO-1 antibody responses as predictive for in Methods A multicenter prospective study was conducted between 2012 and 2021. Patients with resectable cT3-4 cancer were included. Postoperative p53 serially evaluated every 3 months 1 year patients positive preoperative responses. The rate assessed by positivity at 12...
Small bowel duplication in adults is an uncommon congenital anomaly that often presents with nonspecific symptoms, such as abdominal pain, vomiting, or constipation, which complicates diagnosis. Imaging techniques computed tomography (CT) and ultrasonography are commonly used, cine magnetic resonance imaging (MRI) has emerged a promising modality for diagnosing cysts by capturing peristaltic movements. Surgical resection the definitive treatment preventing complications obstruction,...
Background —Because the mechanisms of atherosclerosis or restenosis after angioplasty have been postulated to involve an increase in transforming growth factor (TGF)-β, a selective decrease TGF-β may therapeutic value. Thus, we used ribozyme strategy actively cleave targeted gene selectively inhibit expression. Methods and Results —We constructed oligonucleotides (ONs) sequence that shows 100% homology among human, rat, mouse species. The specificity against was confirmed by inhibition mRNA...
PG production in uterine tissues is important for many physiological processes late pregnancy, including parturition. We examined the expression of PGH2 synthases, cyclooxygenase-1 (COX-1) and COX-2, during using PGF receptor-deficient (FP-/-) mice. Female FP-/- mice are unable to deliver normal fetuses at term, as they do not undergo luteolysis necessary In wild-type mice, COX-1 messenger RNA (mRNA) was expressed endometrial epithelium, myometrium, decidua throughout pregnancy. The mRNA...
Objective: Although interest in diastolic heart failure is growing because of its clinical frequency, little known about this type failure. Our laboratory recently developed a model using Dahl salt-sensitive rat. In model, gene expression angiotensin-converting enzyme and endothelin (ET) system the left ventricle was enhanced at stage without downregulation angiotensin 1a receptor mRNA level. However, roles these humoral systems transition to remain unclear. Methods: Subdepressor doses II 1...
Background— Although lipoprotein(a) (Lp[a]) is a risk factor for atherosclerosis, no study has documented the effects of Lp(a) on angiogenesis. In this study, we examined collateral formation in peripheral arterial disease (PAD) model transgenic mice. addition, feasibility gene therapy by using an angiogenic growth factor, hepatocyte (HGF), to treat PAD presence high Lp(a). Methods and Results— mice, degree natural recovery blood flow after operation was significantly lower than that...
Abstract —Angiogenic growth factors play important roles in angiogenic responses, such as vasculogenesis and angiogenesis response to hypoxia. A novel factor, hepatocyte factor (HGF), has been reported inhibit endothelial cell death. However, its molecular mechanisms are largely unknown. Thus, we studied (1) the effects of HGF on hypoxia-induced apoptosis (2) antiapoptotic actions cells. Severe hypoxia increased death rate human aortic cells, whereas significantly attenuated In addition,...
Persistent exposure to tumor antigens results in exhausted tumor-infiltrating T cells (TILs) that express the immune checkpoint molecules, PD-1 and Tim3, lack anti-tumor immunity. To examine status of TILs ovarian cancer, potential for cytokine production, proliferation cytotoxicity by purified PD-1+ Tim3+ CD8 was assessed. The production IFN-γ TNF-α remained same an intracellular staining assay higher a catch than PD-1- Tim3- TILs. %Ki67+ However, patients with high had poor prognosis. then...
Abstract Purpose Liquid-based cytology is highly useful in oral cytology. However, there are only few reports on the accuracy of this method. The current study aimed to compare liquid-based cytological and histological diagnoses evaluate items that should be considered diagnosis for squamous cell carcinoma. Methods We included 653 patients who underwent both examinations. Data sex, specimen collection region, diagnoses, images were reviewed. Results overall male-to-female ratio was 1:1.18....
In murine osteoblastic MC3T3‐E1 cells which produced prostaglandin E2 as a bone resorption factor, the cyclooxygenase‐2 induction by tumor necrosis factor α (TNFα) was suppressed dexamethasone with an IC 50 of 1 nM. Humulon isolated from hop extract for beer brewing reported previously inhibitor [Tobe, H. et al. (1997) Biosci. Biotech. Biochem. 61, 158–159]. We showed that compound TNFα‐dependent low about 30 nM demonstrated experimentally catalytic activity assay, Northern blot analysis and...