A5067064486- Hepatitis B Virus Studies
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- MicroRNA in disease regulation
- Liver Disease Diagnosis and Treatment
- Spectroscopy Techniques in Biomedical and Chemical Research
- Histone Deacetylase Inhibitors Research
- HIV Research and Treatment
- Lipid metabolism and biosynthesis
- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Hepatitis C virus research
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- RNA Research and Splicing
- Pancreatic function and diabetes
- Autophagy in Disease and Therapy
- Cell death mechanisms and regulation
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
- Digital Imaging for Blood Diseases
- Gut microbiota and health
- Cholangiocarcinoma and Gallbladder Cancer Studies
Italian Institute of Technology
2019-2025
Sapienza University of Rome
2011-2024
University Gastroenterology
2013
Ospedale Maggiore
2013
Istituto Nazionale Genetica Molecolare
2013
University of Milan
2013
Singapore Institute for Clinical Sciences
2013
Inserm
2013
Gleneagles Hospital
2013
Fondazione Andrea Cesalpino
2002-2011
HBV infection remains a leading cause of death worldwide. IFN-α inhibits viral replication in vitro and vivo, pegylated is commonly administered treatment for individuals infected with HBV. The genome contains typical IFN-stimulated response element (ISRE), but the molecular mechanisms by which suppresses have not been established relevant experimental systems. Here, we show that decreasing transcription pregenomic RNA (pgRNA) subgenomic from covalently closed circular DNA (cccDNA)...
Alzheimer's disease (AD), a primary cause of dementia in the aging population, is characterized by extracellular amyloid-beta peptides aggregation, intracellular deposits hyperphosphorylated tau, neurodegeneration and glial activation brain. It commonly thought that lack early diagnostic criteria among main causes pharmacological therapy clinical trials failure; therefore, actual challenge to define new biomarkers non-invasive technologies measure neuropathological changes vivo at...
The HBV covalently closed circular DNA (cccDNA) is organized as a mini-chromosome in the nuclei of infected hepatocytes by histone and non-histone proteins. Transcription from cccDNA RNA replicative intermediate termed pre-genome (pgRNA), critical step for genome amplification ultimately determines rate replication. Multiple evidences suggest that epigenetic modifications, such modifications methylation, participate regulating transcriptional activity cccDNA. Inflammatory cytokines (TNFα,...
Abstract Metformin is a commonly prescribed type II diabetes medication that exhibits promising anticancer effects. Recently, these effects were found to be associated, at least in part, with modulation of microRNA expression. However, the mechanisms by which single modulated microRNAs mediate metformin are not entirely clear and knowledge such process could vital maximize potential therapeutic benefits this safe well-tolerated therapy. Our analysis here revealed expression miR-21-5p was...
The Hepatitis B Virus (HBV) HBx regulatory protein is required for HBV replication and involved in HBV-related carcinogenesis. interacts with chromatin modifying enzymes transcription factors to modulate histone post-translational modifications regulate viral cccDNA cellular gene expression. Aiming identify genes non-coding RNAs (ncRNAs) directly targeted by HBx, we performed a immunoprecipitation sequencing (ChIP-Seq) analyse recruitment on host cell cells replicating HBV.ChIP-Seq high...
The NAD(+)-dependent histone deacetylase hSirT1 regulates cell survival and stress responses by inhibiting p53-, NF-kappaB-, E2F1-dependent transcription. Here we show that the hSirT1/PCAF interaction controls E2F1/p73 apoptotic pathway. represses P1p73 promoter activity in untreated cells inhibits its activation response to DNA damage. hSirT1, PCAF, E2F1 are corecruited vivo on theP1p73 promoter. deacetylates PCAF vitro modulates acetylation vivo. In exposed damage, nuclear NAD(+) levels...
Spontaneous Raman microscopy reveals the chemical composition of a sample in label-free and non-invasive fashion by directly measuring vibrational spectra molecules. However, its extremely low cross section prevents application to fast imaging. Stimulated scattering (SRS) amplifies signal several orders magnitude thanks coherent nature nonlinear process, thus unlocking high-speed applications that provide analytical information elucidate biochemical mechanisms with subcellular resolution....
Introduction Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the progressive loss of motor neuron function. Although ophthalmic deficits are not considered classic symptom ALS, recent studies suggest that changes in retinal cells, similar to those spinal cord neurons, have been observed postmortem human tissues and animal models. Methods In this study, we examined immunofluorescence analysis cell layers sporadic ALS patients post-mortem slices. We...
‘Dysbiosis’ of the adult gut microbiota, in response to challenges such as infection, altered diet, stress, and antibiotics treatment has been recently linked pathological alteration brain function behavior. Moreover, microbiota composition constantly controls microglia maturation, revealed by morphological observations gene expression analysis. However, it is unclear whether functional properties crosstalk with neurons, known shape modulate synaptic development function, are influenced...
Astroblastoma is a rare glial neoplasm more frequent in young female patients, with unclear clinical behaviors and outcomes. The diagnostic molecular alteration rearrangement of the Meningioma 1 (MN1) gene. MicroRNAs (miRNAs) are important gene expression regulators strong implications biological processes. Here, we investigated microRNA expression, regulation, processes correlated to target genes deregulated miRNAs MN1-altered astroblastoma. A cohort 14 tumor samples, histologically...
TP53 belongs to a small gene family that includes, in mammals, two additional paralogs, TP63 and TP73 . The p63 p73 proteins are structurally functionally similar p53 their activity as transcription factors is regulated by wide repertoire of shared unique post‐translational modifications interactions with regulatory cofactors. have important functions embryonic development differentiation but also involved tumor suppression. biology complex since both genes transcribed into variety different...
Gene expression regulation by small interfering RNA (siRNA) holds promise in treating a wide range of diseases through selective gene silencing. However, successful clinical application nucleic acid-based therapy requires novel delivery options. Herein, to achieve efficient negatively charged siRNA duplexes, the internal cavity "humanized" chimeric Archaeal ferritin (HumAfFt) was specifically decorated with cationic piperazine-based compounds (PAs). By coupling these rigid-rod-like amines...
Abstract Modification of histones by lysine methylation plays a role in many biological processes, and it is dynamically regulated several histone methyltransferases demethylases. The polycomb repressive complex contains the H3K27 methyltransferase EZH2 controls dimethylation trimethylation (H3K27me2/3), which trigger gene suppression. JMJD3 UTX have been identified as demethylases that catalyze demethylation H3K27me2/3, turns lead to transcriptional activation. EZH2, extensively studied for...
Abstract Dietary overload of toxic, free metabolic intermediates leads to disrupted insulin signalling and fatty liver disease. However, it was recently reported that this pathway might not be universal: depletion histone deacetylase (HDAC) enhances sensitivity alongside hepatic lipid accumulation in mice, but the mechanistic role microscopic structure effect remains unclear. Here we study Entinostat, a synthetic HDAC inhibitor undergoing clinical trials, on metabolism paradigmatic HepaRG...
Abstract Non-alcoholic fatty liver disease (NAFLD) is a leading cause of chronic disease. Although genetic predisposition and epigenetic factors contribute to the development NAFLD, our understanding molecular mechanism involved in pathogenesis still emerging. Here we investigated possible role microRNAs-STAT3 pathway induction hepatic steatosis. Differentiated HepaRG cells treated with acid sodium oleate (fatty dHepaRG) recapitulated features vesicular steatosis activated cell-autonomous...
Persistent mortality rates of medulloblastoma (MB) and severe side effects the current therapies require definition molecular mechanisms that contribute to tumor progression. Using cultured MB cancer stem cells xenograft tumors generated in mice, we show low expression miR-326 its host gene β-arrestin1 (ARRB1) promotes growth enhancing E2F1 pro-survival function. Our models revealed ARRB1 are controlled by a bivalent domain, since H3K27me3 repressive mark is found at their regulatory region...