A5050912576- Streptococcal Infections and Treatments
- Neonatal and Maternal Infections
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Blood Coagulation and Thrombosis Mechanisms
- Antimicrobial Resistance in Staphylococcus
- Complement system in diseases
- Infective Endocarditis Diagnosis and Management
- Sepsis Diagnosis and Treatment
- Advanced Breast Cancer Therapies
- Pneumonia and Respiratory Infections
- Extracellular vesicles in disease
- Neuroendocrine Tumor Research Advances
- Rabies epidemiology and control
- Ocular Infections and Treatments
- Urticaria and Related Conditions
- Peptidase Inhibition and Analysis
- Retinal Diseases and Treatments
- Bacterial Infections and Vaccines
- Cell Adhesion Molecules Research
- Retinal and Optic Conditions
- Lung Cancer Research Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Tannin, Tannase and Anticancer Activities
- Cancer Cells and Metastasis
- Cancer Research and Treatments
University of Rostock
2016-2025
Institute of Medical Microbiology and Hygiene
2017-2019
Small regulatory RNAs (sRNAs) play a role in the control of bacterial virulence gene expression. In this study, we investigated an sRNA that was identified Streptococcus pyogenes (group A Streptococcus, GAS) but is conserved throughout various streptococci. deletion strain, expression mga, encoding multiple regulator, reduced. Accordingly, transcript and proteome analyses revealed decreased several Mga-activated genes. Therefore, because shown to interact with 5' UTR mga gel-shift assay,...
Severe invasive infectious diseases remain a major and life-threatening health problem. In serious cases, systemic activation of the coagulation cascade is critical complication that associated with high mortality rates. We report here streptokinase, group A streptococcal plasminogen activator, triggers human contact system. Activation system factors at surface Streptococcus pyogenes serotype M49 dependent on streptokinase plasminogen. Our results also show secreted an efficient independent...
Abstract The tannase-producing Gram-positive bacterial species Streptococcus gallolyticus subsp. ( Sgg ) is an opportunistic pathogen of the human gut and strongly associated with colorectal cancer (CRC). A unique feature its ability to degrade tannic acids (TA). TA constitute important part diet known anti-tumorigenic properties. Here, we examined whether able protect tumor cells from toxic effect thus drive tumorigenesis indirectly. Human CRC cell lines (n = 8) were treated increasing...
Streptococcus gallolyticus subsp. gallolyticus, formerly classified as S. bovis biotype I, is an increasing cause of bacteremia and infective endocarditis in the elderly. The physiopathology poorly understood involves immune coagulation systems. In this study, we found that activates human contact system, which turn has two consequences: cleavage high-molecular-weight kininogen (HK) resulting release potent pro-inflammatory peptide bradykinin, initiation intrinsic pathway coagulation. was to...
Histones are small basic proteins and highly conserved among eukaryotes. Their main function is binding, packaging organizing of DNA in the nucleus, but extracellular histones also potent antimicrobial proteins. Here we found that <i>Streptococcus pyogenes - </i>an important human pathogen protects itself from histone-killing by acquisition plasminogen. Plasminogen, bound to streptococcal surface, efficiently prevents histone-mediated killing. Moreover, streptokinase/plasminogen...
Sepsis is associated with coagulation abnormalities, and a high content of intravascular tissue factor (TF) may contribute to the development multisystem organ failure. Circulating microvesicles (MVs) are increased during sepsis characterized by their phosphatidylserine content. It unclear whether MVs—as part host response infection—are beneficial or rather systemic complications in sepsis. In present prospective clinical pilot study, we investigated plasma TF MVs risk multiple failure...
A prominent feature of severe streptococcal infections is the profound inflammatory response that contributes to systemic toxicity. In sepsis dysregulated host involves both immunological and nonimmunological pathways. Here, we report a fatal case an immunocompetent healthy female presenting with toxic shock purpura fulminans caused by group B streptococcus (GBS; serotype III, CC19). The strain (LUMC16) was pigmented hyperhemolytic. Stimulation human primary cells hyperhemolytic LUMC16...
BackgroundHigh-molecular-weight kininogen is a cofactor of the human contact system, an inflammatory response mechanism that activated during sepsis. It has been shown high-molecular-weight contributes to endotoxemia, but not critical for local host defense pneumonia by Gram-negative bacteria. However, some important pathogens, such as Streptococcus pyogenes, can cleave system activation. Whether causally affects antibacterial in S. pyogenes infection, remains unknown.MethodsKininogen...
Sepsis causes an activation of the human contact system, inflammatory response mechanism against foreign surfaces, proteins and pathogens. The serine proteases factor XII plasma kallikrein, are decreased in septic patients, which was previously associated with unfavorable outcome. However, precise mechanisms roles system factors bacterial sepsis poorly understood. We, therefore, studied physiological relevance kallikrein a mouse model experimental sepsis. We show that concentration mice is...
Streptococcus pyogenes uses lactic acid fermentation for the generation of ATP. Here, we analyzed impact a deletion L-lactate dehydrogenase gene ldh on virulence S. M49. While does not cause general growth deficiency in laboratory media, human blood and plasma is significantly hampered. The strain furthermore less virulent Galleria mellonella infection model. We show that leads to decrease activity cysteine protease SpeB, an important secreted factor pyogenes. reduced SpeB caused by hampered...
Abstract Sepsis and septic shock are life-threatening conditions remain an important medical problem, emphasizing the need to identify novel therapeutic approaches. Coagulation dysfunction, hypotension, disturbed microcirculation multiorgan failure occur frequently. These severe result from overwhelming inflammatory response, induced by pathogen damage associated molecular patterns (PAMPs DAMPs) released into bloodstream. In present study, we demonstrated that synthetic Lipopolysaccharid...
Abstract Thromboembolic events are complications in cancer patients and hypercoagulability has been linked to the tissue factor (TF) pathway, making this an attractive target. Here, we investigated effects of chemotherapeutics CDK inhibitors (CDKI) abemaciclib/palbociclib (CDK4/6), THZ-1 (CDK7/12/13), dinaciclib (CDK1/2/5/9) alone combination regimens on TF abundance coagulation. The human colorectal (CRC) cell line HROC173 was treated with 5-FU or gemcitabine stimulate expression. + cells...
The coagulation and contact systems are parts of the innate immune system as they prevent bleeding dissemination pathogens also contribute to microbial killing by inflammatory reactions release antimicrobial peptides. Here, we investigated influence <i>Streptococcus pneumoniae</i> on system. <i>S. (pneumococci), but no other streptococcal species, impairs blood autolysis pneumolysin. Defective results from lysis tissue factor-producing mononuclear cells their procoagulant...
Infections originating from subcutaneous tissues are among the most common invasive infections caused by group A streptococcus (GAS) and associated with systemic coagulation activation. The role of intrinsic factors on GAS virulence has recently been determined, but extrinsic factor VII is unknown. Using a mouse model, in which GAS-sepsis emerges infection, we show that FVII negative acute phase protein. F7 knockdown using antisense oligonucleotides resulted an attenuated activation...