Dietary medicinal plants are among the most sought-after topics in alternative medicine today due to their preventive and healing properties against many diseases.This study aimed extract determine polyphenols from indigenous extracts, i.e., Mentha longifolia, M. arvensis, Tinospora cordifolia, Cymbopogon citratus, Foeniculum vulgare, Cassia absus, Camellia sinensis, Trachyspermum ammi, C. sinensis then evaluate antioxidant, cytotoxicity, antimicrobial properties, besides enzyme inhibition...

Phenols are very soluble in water; as a result, they can pollute massive volume of fresh water, wastewater, groundwater, oceans, and soil, negatively affecting plant germination animal human health. For the detoxification bioremediation phenol biodegradation using novel bacteria isolated from sewage sludge was investigated. Twenty samples (SS) were collected, SS contents cultured mineral salt agar (MSA) containing (500 mg/L). colonies (S1 up to S20) recovered all tested samples. The...

Purslane (Portulaca oleracea L.) is rich in phenolic compounds, protein, and iron. This study aims to produce functional yogurt with enhanced antioxidant, anticancer, antiviral, antimicrobial properties by including safe purslane extract formulation; the was preserved for 30 days at 4 °C, then biochemical fluctuations were monitored. The (PuE) had high compounds flavonoids of 250 56 mg/mL, respectively. Therefore, PuE considerable antioxidant activity, which scavenged 93% DPPH˙, inhibited...

Heavy metals are the major concern of modern age. Among heavy metals, chromium (Cr(VI)) is regarded as a highly toxic metal released largely from leather tanning operations. To remove such high concentrations Cr(VI), an advanced method required urgently. Thus, biosorption using biochar, which organic material produced various sources walnut shell, can be applied successfully for Cr(VI) abatement. The objectives this experiment were remediation shell biochar and checking effect pH, dosage, Cr...

The Agrobacterium-mediated floral dip protocol is the most extensively used transformation method for a model plant Arabidopsis thaliana. Several useful methods Agrobacterium tumefaciens-mediated transformations of are existing, but they time consuming and with low efficiency. Here, we developed transgenic lines TET12p::TET12-RFP in short period enhanced efficiency by using modified applying drought stress after dip. In this protocol, cells carrying recombinant vectors were resuspended...

Abstract Groundwater is an excellent alternative to freshwater for drinking, irrigation, and developing arid regions. Agricultural, commercial, industrial, residential, municipal activities may affect groundwater quantity quality. Therefore, we aimed use advanced methods/techniques monitor the piezometric levels collect samples test their physicochemical biological characteristics. Our results using software programs showed two main types of groundwater: most prevalent was Na–Cl type, which...

Highly pathogenic avian influenza (HPAI) is a serious viral infection that causes massive economic losses in poultry. The current study investigated the HPAI virus prevalence commercial broiler chicken flocks from 2019 to 2022. Organ samples, including trachea, cecal tonsils, spleen, brain, as well tracheal and cloacal swabs, were harvested 111 problematic suffered variable mortalities accompanied with respiratory signs (103 H5-vaccinated 8 nonvaccinated flocks) Egypt during observation...

The purpose of this study is to detect the impact covalent immobilization β-glucosidase (β-Glu) from Thermatoga maritima on sodium alginate-based magnetically recyclable iron nanoparticles (SA-Fe3O4 MNPs) for boosting robustness, facile retrieval, and recycling enzyme. We developed a novel green method amend transformation lactose galacto-oligosaccharides (GOS). interaction Immobilized β-Glu SA-Fe3O4 MNPs were characterized by SDS-PAGE, Transmission electron microscope (TEM) FT-IR...

Valorizing the wastes of food industry sector as additives in foods and beverages enhances human health preserves environment. In this study, pomegranate pomace (PP) was obtained from company Schweppes exposed to production polyphenols fiber-enriched fractions, which were subsequently included a strawberry-yogurt smoothie (SYS). The PP is rich carbohydrates fibers has high water-absorption capacity (WAC) oil-absorption (OAC) values. LC/MS phenolic profile extract indicated that punicalagin...

Selenium is one of the necessary micronutrients needed for enhanced gut microbiota and oxidative stress poultry, so it improves their performance. In this study, Bacillus subtilus DA20 isolate that identified at gene level by PCR was employed to produce eco-friendly selenium nanoparticles (BSeNPs) investigate effects on growth performance, carcass characteristics, blood parameters, Indian River (IR) broiler chickens. The obtained were spherical with size 56 nm net negative charge -22.36 mV;...

This study aimed to isolate, purify, and identify some bacteria from different sources known be contaminated with pesticides evaluate their ability degrade two important pesticides, chlorantraniliprole (CAP), flubendiamide (FBD). In our study, six isolates showed maximum growth in the presence of CAP FBD media as a sole carbon source. The were purified then identified by biochemical morphological tests, MALD-TOF-MS, 16S rRNA techniques, Bacillus subtilis subsp. AZFS3, pumilus AZFS5,...

Industrial pomaces are cheap sources of phenolic compounds and fibers but dumping them in landfills has negative environmental health consequences. Therefore, valorizing these wastes the food industry as additives significantly enhances final product. In this study, citrus pomaces, orange pomace (OP), mandarin (MP), lemon (LP) were collected by a juice company subjected to producing polyphenols fiber-enriched fractions, which included functional yogurt; powder with different levels (1, 3,...

Natural rubber (NR) powder wastes contribute to the pollution of environment and pose a risk human health. Therefore, Escherichia coli AY1 Aspergillus oryzae were used degrade NR in present investigation. The biodegradation was further confirmed using E. A. oryzae’s ability create biofilm, which grew on surface NR. Additionally, biodegraded examined by scanning electron microscopy (SEM), attenuated total reflection–Fourier transform infrared (ATR–FTIR) spectroscopy, gas chromatography–mass...

It is of interest to study the rhizobacteria associated with two different desert wild plants, e.g., Calotropis procera and Senna alexandrina compared bulk soil sample in order identify signatures microbes rhizospheres plants detect influence microbiome drawing architecture. Analysis deep sequencing microbial dataset indicated occurrence 296,642 sequence tags assigned 5,210 OTUs (operational taxonomic units). Species richness control was higher than those either plant's rhizosphere, while...

Biological-based conversion of agricultural residues into bioactive compounds may be considered to the basis for various vital industries. However, finding a suitable microorganism is challenge in bioconversion process. Therefore, this study was conducted find local fungal isolates able convert combination plant biomass organic acids (OAs). Based on their cellulase and phytase activities rock phosphate (RP) solubilization potential, an efficient 15 (named F1 F15) were selected identified by...

Green nanotechnology has attracted attention worldwide, especially in treating cancer and drug-resistant section 6 microbes. This work aims to investigate the anticancer activity of green silver nanoparticles synthesized by Spirulina platensis phycocyanin (SPAgNPs) on two cell lines: Lung line (A-549) breast (MCF-7), compared normal human lung (A138). We also aimed bactericidal against Staphylococcus aureus ATCC29737, Bacillus cereus ATCC11778, Escherichia coli ATCC8379, Klebsiella...

Staphylococcus aureus is one of the high-threat pathogens equipped with a repertoire virulence factors making it responsible for many infections in humans, including foodborne diseases. The present study aims to characterize antibiotic resistance and S. isolates, investigate their cytotoxic effects human intestinal cells (HCT-116). Our results revealed methicillin phenotypes (MRSA) along detection mecA gene (20%) among tested strains. Furthermore, 40% isolates showed strong ability adhesion...

The present study aims at detecting Programmed Cell Death (PCD) related genes that were triggered by exogenous oxalic acid (OA) in tomato (Solanum lycopersicum L.). Reverse transcription polymerase chain reaction (RT-PCR) for the relative abundance of transcripts four PCD-related wild type (WT) plants resulted increased expression levels during time course treatment. results virus induced gene silencing (VIGS) lines corresponding to two MEK2 and SGT1 retarded cell death under OA treatment 24...

The influence of Miswak (Salvadora persica L.) that is made from arak on the oral microbiome signatures 6-to 12-year-old Saudi children was studied.Deep sequencing done for V3-V4 regions bacterial 16S rRNA.Sequence tags were assigned to 291 OTUs (operational taxonomic units) across samples with ≥ 97% similarity.The results indicated factors like ethnic background and/or geographic origin can differences in healthy signatures.The some changes overall structure and diversity microbiome.At...