A5103012306- Inflammasome and immune disorders
- MicroRNA in disease regulation
- interferon and immune responses
- Autophagy in Disease and Therapy
- Signaling Pathways in Disease
- Circular RNAs in diseases
- Immune Response and Inflammation
- Berberine and alkaloids research
- Cancer-related molecular mechanisms research
- Heme Oxygenase-1 and Carbon Monoxide
- Curcumin's Biomedical Applications
- Retinoids in leukemia and cellular processes
- Cardiovascular Disease and Adiposity
- Gout, Hyperuricemia, Uric Acid
- Calpain Protease Function and Regulation
- Peptidase Inhibition and Analysis
- Protease and Inhibitor Mechanisms
- Sirtuins and Resveratrol in Medicine
- Neuropeptides and Animal Physiology
- 3D Printing in Biomedical Research
- Galectins and Cancer Biology
- Mesenchymal stem cell research
- Nuclear Receptors and Signaling
- NF-κB Signaling Pathways
- Toxin Mechanisms and Immunotoxins
First Affiliated Hospital of Wenzhou Medical University
2015-2024
Wenzhou Medical University
2015-2024
XinHua Hospital
2008-2010
Shanghai Jiao Tong University
2009
The inflammatory responses of monocytes/macrophages and the stimulation lipid uptake into these cells by oxidized low density lipoprotein (oxLDL) are critical to initiation development atherosclerosis. Increasing evidence has demonstrated that many microRNAs play important roles in cell proliferation, apoptosis, differentiation accompany responses. However, whether associated with monocyte/macrophage or oxLDL is not yet known. aim present study investigate their potential role...
Emodin has recently been reported to have a powerful antiinflammatory effect, protecting the myocardium against ischemia/reperfusion (I/R) injury. Pyroptosis is proinflammatory programmed cell death that related many diseases. The present study investigated effect of emodin on pyroptosis in cardiomyocytes.Sprague Dawley rats were randomly divided into sham, I/R, and I/R+Emodin groups. I/R model was subjected 30 minutes' ligation left anterior descending coronary artery, followed by 2 hours...
Aims: In the NOD-like receptor (NLR) family, pyrin domain containing 3 (NLRP3) inflammasome is closely related to progression of atherosclerosis. This study aimed assess effects curcumin on NLRP3 in phorbol 12-myristate 13-acetate (PMA)-induced macrophages and explore its underlying mechanism. Methods: Human monocytic THP-1 cells were pretreated with for 1 h subsequently induced PMA 48 h. Total protein was collected Western blot analysis. Cytokine interleukin (IL)-1β release nuclear factor...
MicroRNAs and autophagy play critical roles in cardiac hypoxia/reoxygenation (H/R)-induced injury. Here, we investigated the function of miR-21 regulating identified potential molecular mechanisms involved. To determine role autophagy, H9c2 cells were divided into following six groups: control group, H/R (miR-21+ H/R) (miR-21-negative + (BEZ235+ group BEZ235+ group. The underwent hypoxia for 1 hr reoxygenation 3 hrs. Cell count kit-8 was used to evaluate cell apoptosis analysed by Western...
In coronary arteries, plaque disruption, the major acute clinical manifestations of atherosclerosis, leads to a subsequent cardiac event, such as myocardial infarction (AMI) and unstable angina pectoris (UA). Numerous reports have shown that high expression MMP-9 (matrix metalloproteinase-9), MMP-13 metalloproteinase-13) EMMPRIN (extracellular matrix metalloproteinase induce) in monocyte/macrophage results progression destabilization. Curcumin exerts well-known anti-inflammatory antioxidant...
Primary percutaneous coronary intervention, or thombolytic therapy, provides effective myocardial blood reconstruction in patients with acute infarction to reduce ischemic injury. However, reperfusion can itself induce cardiomyocyte death, termed injury (I/R). Hypoxia/reoxygenation (H/R) induces apoptosis and excessive autophagy among cardiomyocytes, leading cell death. The present study investigated the effect of curcumin, a natural extract from Curcuma longa, on these two cellular...
Sterile inflammation, characterized by a persistent chronic inflammatory state, significantly contributes to the progression of various diseases such as autoimmune, metabolic, neurodegenerative, and cardiovascular disorders. Recent evidence has increasingly highlighted intricate connection between responses diseases, underscoring pivotal role Stimulator Interferon Genes (STING). STING is crucial for secretion type I interferon (IFN) proinflammatory cytokines in response cytosolic nucleic...
Vascular smooth muscle cells (VSMCs) are primarily responsible for vasoconstriction and the regulation of blood pressure1. Pyroptosis, a particular form regulated cell death, is involved in multiple vascular injuries, including hypertensive dysfunction. This pyroptotic death mediated by pore-forming protein Gasdermin D (GSDMD). study was designed to examine direct effect GSDMD on pyroptosis remodeling. Findings revealed that activated Angiotensin (Ang) II- treated aortas. We then showed...
Several kinds of sesquiterpene lactones have been proven to inhibit NF-κB and retard atherosclerosis by reducing lesion size changing plaque composition. The anti-malarial artemisinin (Art) is a pure lactone extracted from the Chinese herb Artemisia annua (qinghao, sweet wormwood). In present study, we demonstrate that inhibits secretion mRNA levels tumor necrosis factor (TNF)-α, interleukin (IL)-1ß, IL-6 in dose-dependent manner phorbol 12-myristate 13-acetate (PMA)-induced THP-1 human...
Rupture of vulnerable atherosclerotic plaques is the leading cause acute myocardial infarction (AMI) and unstable angina pectoris (UA). However, it still lacks an effective therapy to stabilize plaques. Numerous reports have shown that upregulation MMP-9 (matrix metalloproteinase-9) EMMPRIN (extracellular matrix metalloproteinase inducer) in macrophages involved progression development Here we evaluated impact curcumin on expression macrophages. Macrophages were pretreated with or specific...
Coronary heart disease can be effectively prevented by alleviating atherosclerotic plaque progression. Ox-LDL-induced inflammatory response in macrophages is a critical factor the pathophysiology of atherosclerosis. It well known that circular RNAs (circRNAs) are associated with progression several human diseases, such as coronary artery sponging microRNAs (miRNAs), but function and hidden mechanisms circRNAs macrophage inflammation lipid metabolism remain unclear. In our study, we...
1. Overexpression of extracellular matrix metalloproteinase inducer (EMMPRIN) and metalloproteinases (MMPs) by monocytes/macrophages has been proposed to play a significant role in atherosclerotic plaque progression rupture. The aim the present study was explore whether artemisinin, natural extract from Artemisia annua, could decrease EMMPRIN MMP-9 expression phorbol myristate acetate (PMA)-induced macrophages regulating protein kinase (PK) Cδ/c-Jun N-terminal (JNK)/p38/extracellular...
Backgrund/Aims: Ischemia reperfusion (I/R) promotes the severity of cardiomyocyte injury. Long noncoding RNAs (LncRNAs) are key regulators in cardiovascular diseases. However, association between LncRNAs and myocardial I/R injury has not been thoroughly characterized to date. We attempted clarify potential biological role a LncRNA (E230034O05Rik), which we named hypoxia/reoxygenation (H/R) injury-related factor myocytes (HRIM), by investigating differential expression groups exposed either...
Myocardial remodeling caused by angiotensin II (Ang II) is essential for the pathological process of heart failure. Netrin-1, which an axonal guidance cue, has been shown to be involved in inflammatory response, tumorigenesis, and angiogenesis non-neuronal tissues. However, role Netrin-1 cardiac not fully elucidated. The rat cardiomyocyte cell line H9c2 primary neonatal cardiomyocytes were treated with Ang II. Cells transfected siRNA silence expression. Real-time polymerase chain reaction...
Elevated extracellular matrix metalloproteinase inducer (EMMPRIN) and metalloproteinase-9 (MMP-9) in oxidized low density lipoprotein (oxLDL)-induced macrophages leads to the progression of vulnerable plaques by degradation matrix. Our previous report showed that berberine regulates expression both EMMPRIN MMP-9. In addition, P2X7 receptor (P2X7R) upregulation plays a crucial role development atherosclerosis. However, it is unclear whether regulated P2X7R level inhibit MMP-9 expession...
Retinoid X receptor (RXR) plays a central role in the regulation of intracellular signaling pathways. The activation RXR has protective effect on H2O2-induced apoptosis H9c2 ventricular cells rats. But and mechanism activating cardiomyocytes against hypoxia/reoxygenation (H/R)-induced oxidative iniury are still unclear.The model H/R injury was established through hypoxia for 2 hours reoxygenation 4 9-cis-retinoic acid (9-cis RA) obtained as an agonist, HX531 antagonist. Cultured were...