A5080580927- Stress Responses and Cortisol
- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- Neuroendocrine regulation and behavior
- Adipose Tissue and Metabolism
- Tryptophan and brain disorders
- Cell Image Analysis Techniques
- Memory and Neural Mechanisms
- Optical Imaging and Spectroscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Cellular Mechanics and Interactions
- Neuroscience and Neural Engineering
- Adrenal Hormones and Disorders
- Advanced Radiotherapy Techniques
- Retinoids in leukemia and cellular processes
- Neuroinflammation and Neurodegeneration Mechanisms
- Retinal Development and Disorders
- Growth Hormone and Insulin-like Growth Factors
- Single-cell and spatial transcriptomics
- Circadian rhythm and melatonin
- Photoreceptor and optogenetics research
- Sirtuins and Resveratrol in Medicine
Anhui Medical University
2023-2025
First Affiliated Hospital of Anhui Medical University
2023-2025
University of Science and Technology of China
2016-2022
Chinese Academy of Sciences
2020-2022
Hefei National Center for Physical Sciences at Nanoscale
2019-2022
Center for Excellence in Brain Science and Intelligence Technology
2020
The speed of high-resolution optical imaging has been a rate-limiting factor for meso-scale mapping brain structures and functional circuits, which is fundamental importance neuroscience research. Here, we describe new microscopy method Volumetric Imaging with Synchronized on-the-fly-scan Readout (VISoR) high-throughput, high-quality mapping. Combining synchronized scanning beam illumination oblique over cleared tissue sections in smooth motion, the VISoR system effectively eliminates motion...
The Golgi-Cox staining technique is renowned for its ability to delineate neuronal architecture with remarkable precision. However, the traditional protocol's lengthy processing timeline and limited compatibility immunostaining transgenic labeling have hindered widespread adoption in modern neuroscience research. In current study, we found that adjusting incubation temperature 55°C significantly reduced duration a mere 24 h 100 µm-thick sections of mouse brain tissue. Importantly, our...
Abstract Background Corticotropin-releasing hormone (CRH) is an important neuromodulator that widely distributed in the brain and plays a key role mediating stress responses autonomic functions. While distribution pattern of fluorescently labeled CRH-expressing neurons has been studied different transgenic mouse lines, full appreciation broad diversity this population local neural connectivity can only come from integration single-cell morphological information as defining feature. However,...
Abstract Background Tissue-clearing techniques have recently been developed to make tissues transparent for three-dimensional (3D) imaging at different scales, including single-cell resolution. However, current tissue-clearing workflows several disadvantages, complex protocols, time-consuming application, and fluorescence quenching. Additionally, they can be used mainly clearing larger-volume samples, preventing wide easy applicability in conventional experimental approaches. In this study,...
Abstract Stress is increasingly present in everyday life our fast-paced society and involved the pathogenesis of many psychiatric diseases. Corticotrophin-releasing-hormone (CRH) plays a pivotal role regulating stress responses. The tree shrews are highly vulnerable to which makes them promising animal models for studying However, mechanisms underlying their high stress-susceptibility remained unknown. Here we confirmed that cortisol was dominate corticosteroid shrew significantly increased...
Clinical reports suggest a potential link between excess retinoids and development of depression. Although it has been shown that all-trans retinoic acid (ATRA) administration induces behavioral changes, further insight into how ATRA is involved lacking. The hippocampus seems to be major target retinoids, abnormal synaptic plasticity the in We examined two genes associated with function, discs large homolog 2 (DLG2), synapse differentiation-inducing gene protein 1 (SynDIG1) terms hippocampal...
Abstract Corticotropin-releasing factor (CRF) neurons are one of the most densely distributed cell types in central amygdala (CeA), and involved a wide range behaviors including anxiety learning. However, fundamental input circuits patterns CeA-CRF still unclear. Here, we generate monosynaptic-input map onto at single-cell resolution via retrograde rabies-virus system. We find all inputs located 44 nested subregions that directly innervate neurons; them top-down convergent expressing Ca 2+...
In response to stressors, individuals adopt different coping strategies that are essential for survival and form the basis of differential susceptibility stress-related disorders. The corticotropin-releasing factor (CRF) or medial prefrontal cortex (mPFC) has predominantly been studied in stress-coping respectively, while role mPFC CRF neurons is scarcely understood. Here, using morphological electrophysiological approaches, we characterized as a unique subtype inhibitory interneurons...
We describe a new light-sheet microscopy method for fast, large-scale volumetric imaging. Combining synchronized scanning illumination and oblique imaging over cleared, thick tissue sections in smooth motion, our approach achieves high-speed 3D image acquisition of an entire mouse brain within 2 hours, at resolution capable resolving synaptic spines. It is compatible with immunofluorescence labeling, enabling flexible cell-type specific mapping, readily scalable large biological samples such...
Obtaining fine neuron morphology and connections data is extraordinarily useful in understanding the brain's functionality. Golgi staining a widely used method for revealing neuronal morphology. However, Golgi-Cox-stained tissue difficult to image three dimensions lacks cell-type specificity, limiting its use circuit studies. Here, we describe an expansion-based rapidly clearing tissue. The results show that 1 mm thick can be cleared within 6 hours with well preserved signal. At same time,...
Abstract Corticotropin-releasing hormone (CRH) is an important neuromodulator with wide distribution in the brain. Here, we screened CRH-IRES-Cre;Ai32 mouse line to reveal morphologies of individual CRH neurons throughout brain by using fluorescence micro-optical sectioning tomography (fMOST) system. Diverse dendritic and projection fibers were found various regions. Reconstructions showed hypothalamic had smallest somatic volumes simplest branches, several regions shared a bipolar...