A5030824795- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- Circular RNAs in diseases
- Adrenal and Paraganglionic Tumors
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- Mosquito-borne diseases and control
- Neuroblastoma Research and Treatments
- Cancer, Lipids, and Metabolism
- Neuroendocrine Tumor Research Advances
- Lung Cancer Research Studies
- Glioma Diagnosis and Treatment
- HIV Research and Treatment
- Molecular Biology Techniques and Applications
- Extracellular vesicles in disease
- RNA and protein synthesis mechanisms
- Algorithms and Data Compression
- Data Mining Algorithms and Applications
- Transplantation: Methods and Outcomes
- Big Data and Business Intelligence
- RNA regulation and disease
- Hormonal Regulation and Hypertension
National Institute of Allergy and Infectious Diseases
2023-2025
National Institutes of Health
2019-2025
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2019-2024
Indian Association for the Cultivation of Science
2011-2017
National Institute of Arthritis and Musculoskeletal and Skin Diseases
2017
Circular RNAs are new players in regulation of post transcriptional gene expression. Animal genomes express many circular from diverse genomic locations. A recent study has validated a fairly large number human, mouse, and nematode. play crucial role fine tuning the level miRNA mediated expression by sequestering miRNAs. Their interaction with disease associated miRNAs indicates that important for regulation. In this paper we studied potential association (circRNA) human diseases two...
The most advanced monoclonal antibodies (mAbs) and vaccines against malaria target the central repeat region or closely related sequences within Plasmodium falciparum circumsporozoite protein (PfCSP). Here, using an antigen-agnostic strategy to investigate human antibody responses whole sporozoites, we identified a class of mAbs that cryptic PfCSP epitope is only exposed after cleavage subsequent pyroglutamylation (pGlu) newly formed N terminus. This pGlu-CSP not targeted by current...
Long noncoding RNA (lncRNA) influences post-transcriptional regulation by interfering with the microRNA (miRNA) pathways, acting as competing endogenous (ceRNA). These lncRNAs have miRNA responsive elements (MRE) in them, and control miRNAs available for binding their target mRNAs, thus reducing repression of these mRNAs. lnCeDB provides a database human (from GENCODE 19 version) that can potentially act ceRNAs. The putative mRNA targets mapped to AGO clipped regions are collected from...
The CCHC-type zinc finger nucleic acid-binding protein (CNBP/ZNF9) is conserved in eukaryotes and essential for embryonic development mammals. It has been implicated transcriptional, as well post-transcriptional, gene regulation; however, its acid ligands molecular function remain elusive. Here, we use multiple systems-wide approaches to identify CNBP targets function. We used photoactivatable ribonucleoside-enhanced crosslinking immunoprecipitation (PAR-CLIP) 8,420 binding sites on 4,178...
Tumour evolution with acquisition of more aggressive disease characteristics is a hallmark disseminated cancer. Metastatic pancreatic neuroendocrine tumours (PanNETs) in particular may progress from low/intermediate to high-grade disease. The aim this work was understand the molecular mechanisms underlying metastatic progression as well PanNET transformation We performed multi-omics analysis (genome/exome sequencing, total RNA-sequencing and methylation array) 32 longitudinal samples six...
Abstract Microglia cells in the brain play essential role during Japanese Encephalitis Virus (JEV) infection and may lead to change microRNA (miRNA) mRNA profile. These changes together control disease outcome. Using Affymetrix microarray platform, we profiled cellular miRNA expression at multiple time points viral human microglial (CHME3) cells. In silico analysis of data revealed a phased pattern miRNAs expression, associated with JEV replication provided unique signatures infection....
Host-virus interaction via host cellular components has been an important field of research in recent times. RNA interference mediated by short interfering RNAs and microRNAs (miRNA), is a widespread anti-viral defense strategy. Importantly, viruses also encode their own miRNAs. In times miRNAs were identified as key players host-virus interaction. Furthermore, shown to exploit the miRNA networks suite need. The complex cross-talk between viral targets forms environment for pathogenesis....
Many long intergenic noncoding RNAs (lincRNAs) serve as cancer biomarkers for diagnosis or prognostication. To understand the role of lincRNAs in rare neuroendocrine tumors pheochromocytoma and paraganglioma (PCPG), we performed first time in‐depth characterization lincRNA expression profiles correlated findings to clinical outcomes disease. RNA‐Seq data from patients with PCPGs 17 other tumor types The Cancer Genome Atlas published sources were obtained. Differential analysis a...
Detection of potential cross-reaction between a short oligonucleotide sequence and longer (unintended) is crucial for many biological applications, such as high content screening (HCS), microarray nucleotide probes, or interfering RNAs (siRNAs). However, owing to tolerance mismatches gaps in base-pairing with target transcripts, siRNAs could have up hundreds sequences genome, some small mammalian systems been shown affect the levels messenger (off-targets) besides their intended transcripts...
Abstract Some earlier studies have reported an alternative mode of microRNA-target interaction. We detected target regions within mRNA transcripts from AGO PAR-CLIP that did not contain any conventional microRNA seed pairing but only had non-conventional binding sites with 3′ end. Our study 7 set data measured global protein fold change after transfection pointed towards the association 6-mer and 7-mer involving developed a model to predict degree regulation in terms changes number different...
Investigations have revealed that silencing unwanted transcripts or off-targeting can induce false positive phenotype during RNA interference (RNAi)-based gene function study. But still the standard computational approaches towards small interfering (siRNA) off-target minimization fall short in terms of addressing this issue. Some these off-targets may interfere with biochemical pathway being investigated. It also inadvertently target cell's metabolic pathways unquantifiable consequences on...
Metastatic pheochromocytomas and paragangliomas (PPGLs) are rare neuroendocrine tumors associated with poor prognosis limited therapeutic options. Recent advances in oncology-related immunotherapy, specifically targeting of programmed cell death-1 (PD-1)/programmed death-ligand 1 (PD-L1) pathways, have identified a new treatment potential variety tumors, including advanced tumors. Only fraction patients being treated by immune checkpoint inhibitors shown to benefit from it, displaying need...
Abstract Tumour evolution with acquisition of more aggressive disease characteristics is a hallmark disseminated cancer. Metastatic pancreatic neuroendocrine tumours (PanNETs) in particular, show frequent progression from low/intermediate to high-grade disease. To understand the molecular mechanisms underlying this phenomenon, we performed multi-omics analysis 32 longitudinal samples six metastatic PanNET patients. Following MEN1 inactivation, PanNETs exhibit genetic heterogeneity on both...