Courtney Tucker
A5030566634- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- HIV Research and Treatment
- Immunotherapy and Immune Responses
- Malaria Research and Control
- SARS-CoV-2 detection and testing
- Mosquito-borne diseases and control
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Immunology Research
- Influenza Virus Research Studies
- COVID-19 Clinical Research Studies
- Complement system in diseases
- Bacteriophages and microbial interactions
- Trypanosoma species research and implications
- Immune cells in cancer
- Youth Substance Use and School Attendance
- Respiratory viral infections research
- Invertebrate Immune Response Mechanisms
- Child and Adolescent Psychosocial and Emotional Development
National Institute of Allergy and Infectious Diseases
2021-2025
National Institutes of Health
2021-2025
University of America
2022-2025
Catholic University of America
2022-2025
Henry M. Jackson Foundation
2018-2022
Jackson Foundation
2019
Walter Reed Army Institute of Research
2018
The potential for future coronavirus outbreaks highlights the need to broadly target this group of pathogens. We used an epitope-agnostic approach identify six monoclonal antibodies that bind spike proteins from all seven human-infecting coronaviruses. All conserved fusion peptide region adjacent S2' cleavage site. COV44-62 and COV44-79 neutralize alpha- betacoronaviruses, including severe acute respiratory syndrome 2 (SARS-CoV-2) Omicron subvariants BA.2 BA.4/5, albeit with lower potency...
The most advanced monoclonal antibodies (mAbs) and vaccines against malaria target the central repeat region or closely related sequences within Plasmodium falciparum circumsporozoite protein (PfCSP). Here, using an antigen-agnostic strategy to investigate human antibody responses whole sporozoites, we identified a class of mAbs that cryptic PfCSP epitope is only exposed after cleavage subsequent pyroglutamylation (pGlu) newly formed N terminus. This pGlu-CSP not targeted by current...
A look at variant-specific boosters The evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants concern (VOCs) has raised the question whether current COVID-19 vaccines protect against VOCs and if a variant specific vaccine may be needed. Of currently identified VOCs, Delta is believed to most transmissible, whereas Beta appears resistant. Corbett et al . looked effect boosting using either original WA-1 strain or variant–specific booster. Around 6 months after...
Vaccination of nonhuman primates with SARS-CoV-2 spike trimers and AS03 adjuvant induces neutralizing antibodies sufficient to protect from disease.
Humanity has faced three recent outbreaks of novel betacoronaviruses, emphasizing the need to develop approaches that broadly target coronaviruses. Here, we identify 55 monoclonal antibodies from COVID-19 convalescent donors bind diverse betacoronavirus spike proteins. Most targeted an S2 epitope included K814 residue and were non-neutralizing. However, 11 targeting stem helix neutralized betacoronaviruses different lineages. Eight in this group, including six broadest most potent...
Plasmodium falciparum reticulocyte-binding protein homolog 5 (RH5) is the most advanced blood-stage malaria vaccine candidate and being evaluated for efficacy in endemic regions, emphasizing need to study underlying antibody response RH5 during natural infection, which could augment or counteract responses vaccination. Here, we found that RH5-reactive B cells were rare, circulating immunoglobulin G (IgG) short-lived malaria-exposed Malian individuals, despite repeated infections over...
Abstract Adjuvanted soluble protein vaccines have been used extensively in humans for protection against various viral infections based on their robust induction of antibody responses. Here, prefusion-stabilized spike trimers (preS dTM) from the severe acute respiratory syndrome coronavirus (SARS-CoV-2) were formulated with adjuvant AS03 and administered twice to nonhuman primates (NHP). Binding functional neutralization assays systems serology revealed that NHP developed AS03-dependent...
The potential for future coronavirus outbreaks highlights the need to develop strategies and tools broadly target this group of pathogens. Here, using an epitope-agnostic approach, we identified six monoclonal antibodies that bound spike proteins from all seven human-infecting coronaviruses. Epitope mapping revealed conserved fusion peptide region adjacent S2' cleavage site. Two antibodies, COV44-62 COV44-79, neutralize a range alpha beta coronaviruses, including SARS-CoV-2 Omicron...
Neutralizing antibody responses gradually wane after vaccination with mRNA-1273 against several variants of concern (VOC), and additional boost vaccinations may be required to sustain immunity protection. Here, we evaluated the immune in nonhuman primates that received 100 µg vaccine at 0 4 weeks were boosted week 29 (homologous) or mRNA-1273.β (heterologous), which encompasses spike sequence B.1.351 (beta β) variant. Reciprocal ID 50 pseudovirus neutralizing geometric mean titers (GMT) live...
ABSTRACT Plasmodium falciparum RH5 is the most advanced blood-stage malaria vaccine candidate and under evaluation for efficacy in endemic regions, emphasizing need to study underlying antibody response during natural infection. Here, we found that RH5-reactive B cells were rare malaria-exposed individuals despite repeated infections over multiple years. RH5-specific monoclonal antibodies isolated from these extensively mutated but mostly targeted non-neutralizing epitopes, contrast...
ABSTRACT mRNA-1273 vaccine efficacy against SARS-CoV-2 Delta wanes over time; however, there are limited data on the impact of durability immune responses protection. We immunized rhesus macaques at weeks 0 and 4 assessed one year in blood, upper lower airways. Serum neutralizing titers to were 280 34 reciprocal ID 50 6 (peak) 48 (challenge), respectively. Antibody binding also decreased bronchoalveolar lavage (BAL). Four days after challenge, virus was unculturable BAL subgenomic RNA...
Abstract Monocyte-derived macrophages (MDM) are highly permissive to HIV-1 infection potentially due the downregulation of innate factors during differentiation process. The environmental milieu and anti-viral which modulated macrophage differentiation, have been associated with their increased permissiveness infection. Here, we demonstrate that Army Liposome Formulation containing MPLA, QS-21 (ALFQ) activated MDM normally generate a proinflammatory environment upregulated notably APOBEC3A....