A5007770913- Ion channel regulation and function
- Ion Transport and Channel Regulation
- Cardiac electrophysiology and arrhythmias
- Enzyme Structure and Function
- Neuroscience and Neuropharmacology Research
- Protein Kinase Regulation and GTPase Signaling
- Drug Transport and Resistance Mechanisms
- Protein Structure and Dynamics
- Membrane-based Ion Separation Techniques
- Cell Image Analysis Techniques
- Computational Drug Discovery Methods
- RNA and protein synthesis mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Bacterial Genetics and Biotechnology
- SARS-CoV-2 and COVID-19 Research
- Advanced Proteomics Techniques and Applications
- Electron and X-Ray Spectroscopy Techniques
- Metalloenzymes and iron-sulfur proteins
- SARS-CoV-2 detection and testing
- Gene expression and cancer classification
- Nanopore and Nanochannel Transport Studies
- Cancer-related molecular mechanisms research
- Ferroptosis and cancer prognosis
- Click Chemistry and Applications
- Bacillus and Francisella bacterial research
Westlake University
2019-2024
Shantou University
2024
Shantou University Medical College
2024
Xiamen University
2024
Tsinghua University
2018-2019
Center for Life Sciences
2018-2019
Abstract Plasma membrane Ca 2+ -ATPases (PMCAs) are key regulators of global homeostasis and local intracellular dynamics. Recently, Neuroplastin (NPTN) basigin were identified as previously unrecognized obligatory subunits PMCAs that dramatically increase the efficiency PMCA-mediated clearance. Here, we report cryo-EM structure human PMCA1 (hPMCA1) in complex with NPTN at a resolution 4.1 Å for overall 3.9 transmembrane domain. The single helix interacts TM 8-9 -linker TM10 hPMCA1. required...
Abstract The human SLC26 transporter family exhibits various transport characteristics, and member SLC26A9 performs multiple roles, including acting as Cl – /HCO 3 exchangers, channels, Na + transporters. Some mutations of are correlated with abnormalities in respiration digestion systems. As a potential target colocalizing CFTR cystic fibrosis patients, is great value drug development. Here, we present cryo-EM structure the dimer at 2.6 Å resolution. A segment C-terminal end bound to entry...
Gamma delta (γδ) T cells, a unique cell subgroup, are crucial in various immune responses and immunopathology
Abstract In Gram-negative bacteria, phospholipids are major components of the inner membrane and leaflet outer membrane, playing an essential role in forming unique dual-membrane barrier to exclude entry most antibiotics. Understanding mechanisms phospholipid translocation between represents one challenges surrounding bacterial homeostasis. The conserved MlaFEDB complex functions as ABC transporter drive periplasmic protein MlaC. However, mechanism remains elusive. Here we determined three...
The type 2 ryanodine receptor (RyR2) is responsible for releasing Ca 2+ from the sarcoplasmic reticulum of cardiomyocytes, subsequently leading to muscle contraction. Here, we report 4 cryo-electron microscopy (cryo-EM) structures porcine RyR2 bound distinct modulators that, together with our published structures, provide mechanistic insight into regulation. alone induces a contraction central domain that facilitates dilation S6 bundle but insufficient open pore. small-molecule agonist PCB95...
Abstract The pandemic of COVID-19 caused by SARS-CoV-2 continues to spread around the world. Mutant strains are constantly emerging. At present, Omicron variants have become mainstream. In this work, we carried out a systematic and comprehensive analysis reported spike protein antibodies, counting epitopes genotypes these antibodies. We further comprehensively analyzed impact mutations on antibody classified antibodies according their binding patterns. found that H-RBD class were...
The solute carrier 13 (SLC13) family comprises electrogenic sodium ion-coupled anion cotransporters, segregating into ion-sulfate cotransporters (NaSs) and ion-di- and-tricarboxylate (NaDCs). NaS1 NaDC1 regulate sulfate homeostasis oxidative metabolism, respectively. deficiency affects murine growth fertility, while urinary citrate calcium nephrolithiasis. Despite their importance, the mechanisms of substrate recognition transport remain insufficiently characterized. In this study, we...
Abstract Sodium-Potassium Pump (Na + /K -ATPase, NKA) is an ion pump that generates electrochemical gradient of sodium and potassium ions across the plasma membrane by hydrolyzing ATP. During each Post-Albers cycle, NKA exchanges three cytoplasmic for two extracellular through alternating changes between E1 E2 states. Hitherto, several steps remained unknown during complete working cycle NKA. Here, we report cryo-electron microscopy (cryo-EM) structures recombinant human (hNKA) in distinct...
Acinetobacter baumannii is a prevalent nosocomial pathogen that causes serious threat in health care institutions 1 .A. has demonstrated resistance to wide array of antibiotics, including the last-resort colistin or polymyxin B 2-4 .The MlaFEDB-mediated glycerophospholipid (PL) transport was reported play an important role maintaining integrity lipid membrane 2,3 MlaFEDB complex ATP-binding cassette transporter actively translocate phospholipids between inner and periplasmic protein MlaC 4,5...
Abstract K-Ras mutations represent a most prevalent oncogenic alteration in human cancers. Despite of tremendous efforts, it remains big challenge to develop inhibitors that can target the mutants, especially mutants without specific active or charged side chains such as G12V . Here, taking advantage our previous finding Nedd4-1 is bona fide E3 ubiquitin ligase for wild-type Ras proteins, we developed compound XMU-MP-9 promote ubiquitination and degradation various including , significantly...
Abstract The Solute Carrier Family 12 (SLC12) encodes electroneutral cation-chloride cotransporters (CCCs) that are fundamental in cell volume regulation and chloride homeostasis. Dysfunction of CCCs engenders abnormality renal function neuro-system development. Here we presented structure the full length human potassium-chloride co-transporter 2 (KCC2) 3 (KCC3), KCC3 mutants phosphorylation mimic (P-mimic) dephosphorylation (DP-mimic) status, complex with [(DihydroIndenyl)Oxy] Alkanoic acid...
Abstract The type-2 ryanodine receptor (RyR2) is responsible for releasing Ca 2+ from the sarcoplasmic reticulum of cardiomyocytes, subsequently leading to muscle contraction. Here, we report four cryo-EM structures porcine RyR2 bound distinct modulators that collectively provide mechanistic insight into regulation. alone induces a contraction Central domain facilitates dilation S6 bundle, but insufficient open pore. small molecule agonist PCB95 helps overcome barrier opening. FKBP12.6...