A5043213519- Cancer Genomics and Diagnostics
- Gut microbiota and health
- Colorectal Cancer Treatments and Studies
- Single-cell and spatial transcriptomics
- Advanced biosensing and bioanalysis techniques
- Molecular Biology Techniques and Applications
- Electrochemical Analysis and Applications
- Lung Cancer Treatments and Mutations
- Economic and Financial Impacts of Cancer
- Fatty Acid Research and Health
- Genomics and Phylogenetic Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Biosensors and Analytical Detection
- Environmental DNA in Biodiversity Studies
- Genomic variations and chromosomal abnormalities
- Analytical Chemistry and Sensors
- Breast Cancer Treatment Studies
- Mycobacterium research and diagnosis
- Radiomics and Machine Learning in Medical Imaging
- Colorectal Cancer Surgical Treatments
- Salivary Gland Tumors Diagnosis and Treatment
- Marine animal studies overview
- Nosocomial Infections in ICU
- Child and Adolescent Health
- Neuroblastoma Research and Treatments
University of Leeds
2015-2024
St James's University Hospital
2016-2024
Wellcome Trust
2016-2023
Kelowna General Hospital
2013
Objective Omega-3 polyunsaturated fatty acids (PUFAs) have anticolorectal cancer (CRC) activity. The intestinal microbiota has been implicated in colorectal carcinogenesis. Dietary omega-3 PUFAs alter the mouse microbiome compatible with antineoplastic Therefore, we investigated effect of PUFA supplements on faecal middle-aged, healthy volunteers (n=22). Design A randomised, open-label, cross-over trial 8 weeks’ treatment 4 g mixed eicosapentaenoic acid/docosahexaenoic acid two formulations...
Abstract HER2 overexpression/amplification is linked to trastuzumab response in breast/gastric cancers. One suggested anti‐ EGFR resistance mechanism colorectal cancer ( CRC ) aberrant MEK–AKT pathway activation through up‐regulation. We assessed ‐amplification/overexpression stage II–III and IV patients, assessing relationships KRAS / BRAF outcome. Pathological material was obtained from 1914 patients the QUASAR trial 1342 trials FOCUS PICCOLO ). Tissue microarrays were created for...
Single-cell RNA sequencing has revolutionized our understanding of cellular heterogeneity, but routine methods require cell lysis and fail to probe the dynamic trajectories responsible for state transitions, which can only be inferred. Here, we present a nanobiopsy platform that enables injection exogenous molecules multigenerational longitudinal cytoplasmic sampling from single its progeny. The technique is based on scanning ion conductance microscopy (SICM) and, as proof concept, was...
Adoption of new technology in both basic research and clinical settings requires rigorous validation analytical performance. The OncoScan® FFPE Assay is a multiplexing tool that offers genome-wide copy number loss heterozygosity detection, as well identification frequently tested somatic mutations. In this study, 162 formalin fixed paraffin embedded samples, representing six different tumour types, were profiled triplicate across three independent laboratories. assay data was then analysed...
The microbiome is implicated in the pathogenesis of inflammatory bowel disease (IBD) and irritable syndrome (IBS). Whether a distinct profile associated with reporting IBS-type symptoms IBD patients uncertain. We aimed to resolve this issue using cross-sectional study design. Using clinical activity indices, Rome III criteria for IBS fecal calprotectin levels, we divided into 4 groups: symptoms, quiescent disease, occult inflammation, active disease. A16S rRNA analysis was performed...
Abstract Marine mammal foraging grounds are popular focal points for marine protected area (MPA) implementation, despite being temporally dynamic, requiring continuous monitoring to infer prey availability and abundance. distributions assumed be driven by their in areas, but limited understanding of often prevents us from exploring how shifting impacts both seasonal long‐term distributions. Environmental DNA (eDNA) metabarcoding could enhance the habitat use relation through simultaneous...
The influence of EGFR pathway mutations on cetuximab-containing rectal cancer preoperative chemoradiation (CRT) is uncertain. In a prospective phase II trial (EXCITE), patients with magnetic resonance imaging (MRI)-defined non-metastatic adenocarinoma threatening/involving the surgical resection plane received pelvic radiotherapy concurrent capecitabine, irinotecan and cetuximab. Resection was recommended 8 weeks later. primary endpoint histopathologically clear (R0) margin. Pre-planned...
Aims With a growing interest in the influence gut microbiome has on development of colorectal cancer (CRC), we investigated feasibility and stability isolating typing microbial DNA from guaiac faecal occult blood test (gFOBt) cards. This future potential to screen populations present confirmed neoplasia cases with aims predict presence CRC. Methods Fresh stool samples three healthy volunteers were applied gFOBt was extracted both cards fresh samples. A series additional prepared one...
RPGR ORF15 is an exon present almost exclusively in the retinal transcript of RPGR. It purine-rich, repetitive and notoriously hard to sequence, but a hotspot for mutations causing X-linked retinitis pigmentosa. Long-read nanopore sequencing on MinION Flongle flow cells was used sequence genomic DNA from patients with inherited dystrophy. A cell wash kit increase yield. Findings were confirmed by PacBio SMRT long-read sequencing. We showed that successfully reads through 2 kb PCR-amplified...
The importance of accurate and affordable mutation calling in fixed pathology samples is becoming increasingly important as we move into the era personalised medicine. Affymetrix OncoScan® Array platform designed to produce actionable calls archival material. We compared made using OncoScan with a custom PCR panel followed by next-generation sequencing (NGS), order benchmark sensitivity specificity large cohort tumour samples. 392 fixed, clinical were sequenced, encompassing 641 regions, 403...
Clostridium difficile infection (CDI) is a major cause of nosocomial diarrhea associated with antimicrobial-mediated dysbiosis. Dysbiosis may be perpetuated by antibiotic (AB) CDI therapy, leading to recurrent CDI. Ridinilazole (RIDI) has very narrow activity against certain clostridia. We measured faecal microbiomes Phase 2 subjects randomised 10 days RIDI or fidaxomicin (FDX) for Fecal samples (27 patients) were obtained at study entry (DM1-D1), Day (D2-D3), 5 (D4–5), 7 (D5–7), (D9–10), 12...
ABSTRACT Single-cell RNA sequencing has revolutionised our understanding of cellular heterogeneity, but whether using isolated cells or more recent spatial transcriptomics approaches, these methods require isolation and lysis the cell under investigation. This provides a snapshot transcriptome from which dynamic trajectories, such as those that trigger state transitions, can only be inferred. Here, we present nanobiopsy: platform enables simultaneous labelling sampling single without killing...
Abstract Objectives Copy number (CN) and somatic mutation (SM) analysis in tumors is rapidly gaining importance cancer management as a tool for differential diagnosis, determination of prognosis, selection therapeutic. Genome-wide copy LOH detection well panel frequently tested mutations can be detected with OncoScan® FFPE Assay Kit. We report on validation study OncoScan Kit CN SM data by orthogonal technologies (FISH NGS, respectively) to estimate the sensitivity specificity parameters...