A5079741475- Iron Metabolism and Disorders
- Hemoglobinopathies and Related Disorders
- Immune cells in cancer
- Tryptophan and brain disorders
- Phagocytosis and Immune Regulation
- Erythropoietin and Anemia Treatment
- Immune Response and Inflammation
- Trace Elements in Health
- Long-Term Effects of COVID-19
- Vitamin C and Antioxidants Research
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Cancer Research and Treatments
- Erythrocyte Function and Pathophysiology
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Heme Oxygenase-1 and Carbon Monoxide
- Peroxisome Proliferator-Activated Receptors
- COVID-19 Clinical Research Studies
- Superconductivity in MgB2 and Alloys
- Autophagy in Disease and Therapy
- Immunotherapy and Immune Responses
- Antibiotic Resistance in Bacteria
- Neuroinflammation and Neurodegeneration Mechanisms
- Child Nutrition and Water Access
- Peptidase Inhibition and Analysis
University Hospital Cologne
2024-2025
University of Cologne
2024-2025
Innsbruck Medical University
2018-2024
Universität Innsbruck
2018-2024
Abstract Aims Imbalances of iron metabolism have been linked to the development atherosclerosis. However, subjects with hereditary haemochromatosis a lower prevalence cardiovascular disease. The aim our study was understand underlying mechanisms by combining data from genome-wide association analyses in humans, CRISPR/Cas9 genome editing, and loss-of-function studies mice. Methods results Our analysis Global Lipids Genetics Consortium (GLGC) dataset revealed that single nucleotide...
Anemia is a major health issue and associated with increased morbidity. Iron deficiency anemia (IDA) the most prevalent, followed by of chronic disease (ACD). IDA ACD often co-exist, challenging diagnosis treatment. While iron supplementation first-line therapy for IDA, its optimal route administration efficacy different repletion strategies in are elusive. Female Lewis rats were injected group A streptococcal peptidoglycan-polysaccharide (PG-APS) to induce inflammatory arthritis and/or...
Here we show that dopamine increases bacterial iron incorporation and promotes Salmonella Typhimurium growth both in vitro vivo . These observations suggest the potential hazards of pharmacological catecholamine administration patients with sepsis but also inhibition acquisition might provide a useful approach to antimicrobial therapy.
Iron plays an important role in host–pathogen interactions, being essential element for both pathogen and host metabolism, but also by impacting immune cell differentiation anti-microbial effector pathways. has been implicated to affect the of T lymphocytes during inflammation, however, so far underlying mechanism remained elusive. In order study iron we here investigated how dietary supplementation affects function outcome a model chronic infection with intracellular bacterium Salmonella...
Post-infectious fatigue is a common complication that can lead to decreased physical efficiency, depression, and impaired quality of life. Dysbiosis the gut microbiota has been proposed as contributing factor, gut-brain axis plays an important role in regulating mental health. This pilot study aimed investigate severity well life 70 patients with post-infectious who received multi-strain probiotic preparation or placebo double-blind, placebo-controlled trial. Patients completed...
Arginase 1 (ARG1) is a cytosolic enzyme that cleaves L-arginine, the substrate of inducible nitric oxide synthase (iNOS), and thereby impairs control various intracellular pathogens. Herein, we investigated role ARG1 during infection with Salmonella enterica serovar Typhimurium (S.tm). To study impact on infections in vitro, bone marrow-derived macrophages (BMDM) from C57BL/6N wild-type, ARG1-deficient Tie2Cre+/−ARG1fl/fl NRAMPG169 mice were infected S.tm. In wild-type BMDM, was induced by...
Around 10% of people who had COVID-9 infection suffer from persistent symptoms such as fatigue, dyspnoea, chest pain, arthralgia/myalgia, sleep disturbances, cognitive dysfunction and impairment mental health. Different underlying pathomechanisms appear to be involved, in particular inflammation, alterations amino acid metabolism, autonomic gut dysbiosis.As routine tests are often inconspicuous patients with Long COVID (LC), similarly suffering myalgic encephalomyelitis/chronic fatigue...
Abstract Background NR2F6 has been proposed as an alternative cancer immune checkpoint in the effector T cell compartment. However, a realistic assessment of vivo therapeutic potential requires acute depletion. Methods Employing primary cells isolated from Cas9-transgenic mice for electroporation chemically synthesized sgRNA, we established CRISPR/Cas9-mediated knockout protocol Nr2f6 mouse cells. Results Analyzing these CRISPR/Cas9 cells, reproducibly observed hyper-reactive phenotype upon...
Iron is both, an essential compound for many metabolic processes, and iron deficiency can impact on the proliferation of cells including lymphocytes but also tumor cells. On other hand, excess iron-catalyzed radical formation induce cellular toxicity which has been previously demonstrated T in hereditary overload. Despite these interconnections, little known effects clinically approved intravenous supplements curing cancer-related anemia, cell differentiation, proliferation, anti-tumor...
Iron is an essential nutrient for immune cells and microbes, therefore the control of its homeostasis plays a decisive role infections. Moreover, iron affects metabolic pathways by modulating translational expression key tricarboxylic acid cycle (TCA) enzyme mitochondrial aconitase energy formation mitochondria. Recent data provide evidence re-programming including macrophages during infection which centrally controlled mTOR. We herein studied effects perturbations on profiles in upon with...
Background Klebsiella pneumoniae (KP) is a major cause of hospital-acquired infections, such as pneumonia. Moreover, it classified pathogen concern due to sprawling anti-microbial resistance. During infection, the gram-negative capable establishing an intracellular niche in macrophages by altering cellular metabolism. One factor critically affecting host-pathogen interaction availability essential nutrients, like iron, which required for KP proliferate but also modulates immune effector...
Macrophages are at the center of innate immunity and iron metabolism. In case an infection, macrophages adapt their cellular metabolism to deprive from invading bacteria combat intracellular bacterial proliferation. A concise evaluation content upon infection with pathogens diverse stimuli is necessary identify underlying mechanisms concerning homeostasis in macrophages. For characterization levels during we established vitro model where murine macrophage cell line J774A.1 infected
Iron is an essential component for metabolic processes, including oxygen transport within hemoglobin, tricarboxylic acid (TCA) cycle activity, and mitochondrial energy transformation. deficiency can thus lead to dysfunction eventually result in iron anemia (IDA), which affects approximately 1.5 billion people worldwide. Using a rat model of IDA induced by phlebotomy, we studied the effects on respiration peripheral blood mononuclear cells (PBMCs) liver. Furthermore, evaluated whether...
Priming of macrophages with interferon-gamma (IFNγ) or interleukin-4 (IL-4) leads to polarisation into pro-inflammatory anti-inflammatory subtypes, which produce key enzymes such as inducible nitric oxide synthase (iNOS) and arginase 1 (ARG1), respectively, in this way determine host responses infection. Importantly, L-arginine is the substrate for both enzymes. ARG1 upregulation associated increased pathogen load different infection models. However, while differentiation IL-4 impairs...
Salmonella enterica serovar Typhimurium (S. Typhimurium) is a Gram-negative, facultative intracellular bacterium, which causes gastrointestinal disorders in humans, and systemic, typhoid fever-like infections mice. Our current knowledge regarding the involvement of cellular humoral immunity defense from S. largely based on animal models with attenuated strains. Cells innate immune system act as one first barriers bacteria. We established robust experimental model for characterization these...
Macrophages are important for host defense against intracellular pathogens like Salmonella and can be differentiated into two major subtypes. M1 macrophages, which pro-inflammatory induce antimicrobial immune effector mechanisms, including the expression of inducible nitric oxide synthase (iNOS), M2 exert anti-inflammatory functions express arginase 1 (ARG1). Through process phagocytosis, macrophages contain, engulf, eliminate bacteria. Therefore, they one first lines Salmonella. Infection...
Abstract Research into the molecular basis of disease trajectory and Long-COVID is important to get insights toward underlying pathophysiological processes. The objective this study was investigate inflammation-mediated changes metabolism in patients with acute COVID-19 infection throughout a one-year follow up period. enrolled 34 moderate severe admitted University Clinic Innsbruck early 2020. dynamics multiple laboratory parameters (including inflammatory markers [C-reactive protein (CRP),...
Macrophages are at the center of innate immunity and main target cells intracellular pathogen
Alcohol-related liver disease (ALD) is a global healthcare challenge with limited treatment options. 24-Norursodeoxycholic acid (NorUDCA) synthetic bile anti-inflammatory properties in experimental and human cholestatic diseases. In the present study, we explored efficacy of norUDCA ALD.