DNA topoisomerases are essential to modulate topology during various cellular genetic processes. The expression and distinct prognostic value of topoisomerase isoforms in non-small-cell lung cancer (NSCLC) is not well established. In the current study, we have examined mRNA by using Oncomine analysis investigated their via Kaplan-Meier plotter database NSCLC patients. Our indicated that level was higher compared with normal tissues. Especially, high two isoforms, TOP2A TOP3A, found be...

Abstract Cancer side-population (SP) represents a sub-population of stem-like cancer cells that have an important role in drug resistance due to their high expression the ATP-binding cassette transporter ABCG2 involved export. Auranofin (AF), clinical gold complex is used treatment rheumatoid arthritis, has been reported inducing tumor antiproliferation. However, whether AF can impact SP remains unclear. Our study showed caused depletion and downregulation stem cell markers, impaired ability...

Cancer stem cells (CSCs) are thought to play an important role in tumor recurrence and drug resistance, present a major challenge cancer therapy. The microenvironment such as growth factors, nutrients oxygen affect CSC generation proliferation by providing the necessary energy sources signals. side population (SP) analysis has been used detect stem-like cell populations based on their high expression of ABCG2 that exports Hoechst-33342 certain cytotoxic drugs from cells. purpose this...

Nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (NOX) are frequently deregulated in several human malignancies, including gastric cancer (GC). NOX-derived reactive oxygen species have been reported to contribute carcinogenesis and progression. However, the expression prognostic role of individual NOX GC patients remain elusive.We investigated genetic alteration mRNA family via cBioPortal, Human Protein Atlas, Oncomine databases. Furthermore, we evaluated value distinct through...

Abstract Many circRNAs are involved in the carcinogenesis of breast cancer (BCa) through transcription microRNAs (miRNAs) and mRNAs. This study investigated circRBM33 regulation miR-542-3p/hypoxia-inducible factor-1α (HIF-1α) axis BCa. BCa clinical tissue samples were collected to test differential expressions circRBM33, miR-542-3p, HIF-1α. MCF-7 cells subjected normoxia or hypoxia transfected with plasmids that regulated CircRBM33, HIF-1α expression levels. Glycolysis was evaluated by...

Abstract Thrombospondin-2 (THBS2) is a secreted protein overexpressed in numerous cancers and may function as diagnostic tumor marker. The objective of the present study was to investigate performance serum THBS2 early stage non-small-cell lung cancer (NSCLC). Serum Cyfra21-1 level were evaluated blood samples 112 patients from NSCLC groups 51 healthy control (HC) groups. Receiver operator characteristic (ROC) curves used evaluate significance. significantly up-regulated compared with...

Abstract Purpose The underlying mechanisms of hepatocellular carcinoma (HCC) have not been fully investigated, and effective biomarkers for HCC are still needed to be explored. Therefore, our study sought thoroughly examine the clinical significance biological functions ribosomal protein L32 (RPL32) in by coupling bioinformatic methods with experimental analysis. Methods To determine RPL32 , analyses were performed expression patient samples correlate survival rates, genetic alterations,...

The ubiquitin‑proteasome pathway is an important protein degradation regulatory system in cells. This also a reversible process that strictly regulated, and the regulation of deubiquitinating enzymes (DUBs) represents facet process. Ovarian tumor‑associated proteases domain‑containing proteins (OTUDs), as subfamily within DUB family, serve role mechanisms several biological processes, through gene transcription, cell cycle, immune response, inflammation tumor growth may be diagnosis various...

To investigate the protective effects and mechanism of antioxidant TBHQ on renal damage caused by doxorubicin chemotherapy in mice with hepatic cancer.Cell H22 cancer which was subcultured for three times subcutaneously transplanted to groin right lower limb 45 SPF Kunming establish tumor model. The group intervention received intraperitoneal injection ADM (1 mg/kg·0.2 mL/2 d). model control normal saline (NS) same volume at time. 1% added into diet group. Seven weeks later, morning urines...

There is a need for efficient, convenient, and inexpensive methods to accurately diagnose the clinical stage of lung cancer evaluate efficacy chemotherapy in patients with cancer. Although growth/differentiation factor 15 (GDF)-15 has great potential as tumor marker, supporting evidence still lacking. In this study, we aimed analyze relationship between serum GDF15 concentration characteristics cancer, assess value diagnosis curative effect chemotherapy.The study comprised 160 participants...

Lung adenocarcinoma (LUAD) is the most common subtype of non-small cell lung cancer (NSCLC) and characterized by its heterogeneity poor prognosis. The role ribosomal proteins RPLP0, RPLP1 RPLP2 in multiple cancers has been implicated. However, their function LUAD correlation with prognosis remains elusive. In this study, we performed a comprehensive bioinformatic analysis impact these on LUAD. Our findings reveal that are overexpressed LUAD, which likely attributed to abnormal copy number...

Both V79 and As/R28A cells (an arsenite-resistant Chinese hamster cell variant) show increased resistance to toxic concentrations of arsenite after pretreatment with a nontoxic concentration. The induced tolerance can be completely inhibited by actinomycin D or cycloheximide. Pretreatment heat shock (45 degrees C, 10 min) resulted in clear thermotolerance both lines but failed induce either line. not cells. These results are consistent model whereby the signal for induction involves binding...

Backgrounds: Hepatocellular carcinoma (HCC) is a major type of death-causing cancer whose pathological mechanisms are not fully understood. In addition, the identification effective biomarkers for HCC prognosis in emergency. Although variety studies have shown that Complement C11 binding protein (C1QBP) may play tumor-promoting or tumor-suppressive role cancer, functions and C1QBP progression under-investigating. Methods results: Bioinformatic approaches were employed checking expression...

CerSs (ceramide synthases), a group of enzymes that catalyze the formation ceramides from sphingoid base and acyl-CoA substrates. As far, six types (CerS1-CerS6) have been found in mammals. Each these unique characteristics, but maybe more noteworthy is ability individual CerS isoform to produce ceramide with characteristic acyl chain distribution. key regulators sphingolipid metabolism, highlight their characteristics emerging roles regulating programmed cell death, cancer many other...

Glioma is a common tumor originating from the glial cells of brain. Immune checkpoint inhibitors can potentially be used to treat gliomas, although no drug currently approved.The expression levels immune genes in glioma and normal tissues, their correlation with IDH mutation status complete 1p/19q codeletion, were analyzed using The Cancer Genome Atlas (TCGA) Chinese (CGGA) databases. Survival analyses conducted CGGA database. Protein-protein interaction functional enrichment performed via...

Excision repair cross-complementation group 6 like (ERCC6L), a polo-like kinase 1 (PLK1)-interacting checkpoint helicase, confers high risk of cancer and enhances the progression variety cancers. The present investigation aimed to elucidate pan-cancer expression patterns ERCC6L examine possibility using this gene for patient diagnosis prognosis.The in normal patients at various clinical stages were explored based on TCGA datasets. Subsequently, Bioinformatics techniques then used analyze...

Hepatocellular carcinoma (HCC) ranks third on the list of leading cause for cancer death globally. The treatment HCC patients is unsatisfactory. However, traditional Chinese medicine Chebulae Fructus has potential efficacy in HCC.We mined active ingredients and its main targets from Traditional Medicine Systems Pharmacology database. HCC-related datasets were downloaded Cancer Genome Atlas database differentially expressed genes (DEGs) obtained by differential expression analysis. Top10...

Recent studies have indicated that dysregulation of the Hippo/Yes-associated protein (YAP) axis is associated with tumor progression and therapy resistance in various cancer types, including lung adenocarcinoma (LUAD). Understanding regulation Hippo signaling LUAD great significance. Elevated levels TRIB3, a pseudo kinase, been observed certain malignancies are an unfavorable prognosis. Our research aims to investigate whether increased TRIB3 enhance malignant characteristics cells through...