A5060598034- Marine Toxins and Detection Methods
- Graphene and Nanomaterials Applications
- Nanoparticles: synthesis and applications
- Marine Biology and Environmental Chemistry
- Environmental Toxicology and Ecotoxicology
- Nicotinic Acetylcholine Receptors Study
- Graphene research and applications
- Marine Sponges and Natural Products
- Ion channel regulation and function
- Protist diversity and phylogeny
- Acute Lymphoblastic Leukemia research
- Carbon and Quantum Dots Applications
- Environmental Chemistry and Analysis
- Advancements in Transdermal Drug Delivery
- Pluripotent Stem Cells Research
- Redox biology and oxidative stress
- 3D Printing in Biomedical Research
- Microplastics and Plastic Pollution
- Heavy Metal Exposure and Toxicity
- Mycotoxins in Agriculture and Food
- Advanced Nanomaterials in Catalysis
- CRISPR and Genetic Engineering
- Gold and Silver Nanoparticles Synthesis and Applications
- Glutathione Transferases and Polymorphisms
- Reproductive System and Pregnancy
University of Trieste
2016-2025
IRCCS Materno Infantile Burlo Garofolo
2019
Creative Research Enterprises (United States)
2019
Nara Medical University
2019
GTx (United States)
2016
Two-dimensional (2D) materials have attracted tremendous interest ever since the isolation of atomically thin sheets graphene in 2004 due to specific and versatile properties these materials. However, increasing production use 2D necessitate a thorough evaluation potential impact on human health environment. Furthermore, harmonized test protocols are needed with which assess safety The Graphene Flagship project (2013-2023), funded by European Commission, addressed identification possible...
Abstract Impressive properties make graphene-based materials (GBMs) promising tools for nanoelectronics and biomedicine. However, safety concerns need to be cleared before mass production of GBMs starts. As skin, together with lungs, displays the highest exposure GBMs, it is fundamental importance understand what happens when get in contact skin cells. The present study was carried out on HaCaT keratinocytes, an vitro model toxicity, which effects four were evaluated: a few layer graphene,...
Titanium dioxide nanoparticles (TiO2NPs) suspensions (concentration 1.0 g/L) in synthetic sweat solution were applied on Franz cells for 24 h using intact and needle-abraded human skin. content into skin receiving phases was determined. Cytotoxicity (MTT, AlamarBlue® propidium iodide, PI, uptake assays) evaluated HaCat keratinocytes after h, 48 seven days of exposure. After exposure, no titanium detectable solutions both damaged found the epidermal layer exposure (0.47 ± 0.33 μg/cm2) while...
Graphene based nanomaterials induce a reactive oxygen species-mediated mitochondrial depolarization, caused by the activation of NADH dehydrogenase and xanthine oxidase.
Graphene is the first 2D atomic crystal, and its isolation heralded a new era in materials science with emergence of several other atomically thin displaying multifunctional properties. The safety assessment often something an afterthought, but case graphene, initial characterization material was soon followed by potential impact on living systems. Flagship project addressed health environmental aspects graphene materials, providing instructive lesson interdisciplinarity - from to biology....
Abstract The Graphene Flagship aimed at demonstrating the viability and performance of graphene in e-textile applications where mechanical properties such as flexibility stretchability well sustainability have a crucial role. In this perspective we will discuss work on GRM-containing conductive textile fibres, yarns, fabrics for e-textiles GRM-based functionalities applications. Particular focus is put fabrication textiles through two different methods, namely printing direct incorporation...
Graphene-related materials (GRMs) are subject to intensive investigations and considerable progress has been made in recent years terms of safety assessment. However, limited information is available concerning the hazard potential GRM-containing products such as graphene-reinforced composites. In present study, we conducted a comprehensive investigation biological effects particles released through an abrasion process from reduced graphene oxide (rGO)-reinforced composites polyamide 6...
We assessed the immunomodulatory and anti-inflammatory effects of 9,11-dihydrogracilin A (DHG), a molecule derived from Antarctic marine sponge Dendrilla membranosa. used in vitro vivo approaches to establish DHG properties. Human peripheral blood mononuclear cells (PBMC) human keratinocytes cell line (HaCaT cells) were as system, whereas model murine cutaneous irritation was adopted for studies. observed that reduces dose dependently proliferative response viability mitogen stimulated PBMC....
Besides inhalation, skin contact may be considered one of the most relevant exposure routes to graphene-based materials (GBMs). However, very few data on cutaneous toxicity these are available, so far. This study is focused irritation potential a panel GBMs: few-layer graphene (FLG), exfoliated by ball milling graphite, FLG ultrasonication using sodium dodecyl sulfate (FLG-SDS) or dodecylbenzenesulfonate (FLG-SDBS), CVD-graphene, obtained chemical vapor deposition, oxide (GO) and reduced GO...
Harmful algal blooms are an expanding phenomenon negatively impacting human health, socio-economic welfare, and ecosystems. Such events increase the risk of marine organisms' exposure to toxins with consequent ecological effects. In this frame, objective study was investigate ecotoxicological potential three globally distributed dinoflagellate (okadaic acid, OA; dinophysistoxin-1, DTX-1; dinophysistoxin-2, DTX-2) using Artemia franciscana as a model organism zooplankton. Each toxin (0.1-100...
Skin absorption and toxicity on keratinocytes of cobalt oxide nanoparticles (Co3O4NPs) have been investigated. Co3O4NPs are commonly used in industrial products biomedicine. There is evidence that these can cause membrane damage genotoxicity vitro, but no data available their skin cytotoxicity keratinocytes. Two independent 24 h vitro experiments were performed using Franz diffusion cells, intact (experiment 1) needle-abraded human 2). at a concentration 1000 mg/L physiological solution as...
Glucocorticoids (GCs) exert their effects through regulation of gene expression after activation in the cytoplasm glucocorticoid receptor (GR) encoded by NR3C1 gene. A negative feedback mechanism resulting GR autoregulation has been demonstrated binding activated to intragenic sequences called GRE-like elements, contained The long noncoding RNA growth arrest–specific transcript 5 (GAS5) interacts with suppressing its transcriptional activity. aim this study was evaluate possible role GAS5...
This study provides the first evaluation of cytotoxic effects recently identified palytoxin (PLTX) analog, ovatoxin-a (OVTX-a), major toxin produced by Ostreopsis cf. ovata in Mediterranean Sea. Its increasing detection during blooms and seafood highlights need to characterize its toxic set up appropriate methods. OVTX-a is about 100 fold less potent than PLTX reducing HaCaT cells viability (EC50 = 1.1 × 10(-9) M vs 1.8 10(-11) M, MTT test) agreement with a reduced binding affinity (Kd 1.2...
In September 2015, a massive occurrence of the Ostreopsis species was recorded in central Adriatic Kaštela Bay. order to taxonomically identify responsible for this event and determine their toxin profile, cells collected seawater from benthic macroalgae were analyzed. Conservative taxonomic methods (light microscopy SEM) molecular (PCR-based assay) allowed identification cf. ovata associated with Coolia monotis. The abundance O. reached 2.9 × 104 L−1 seawater, while on macroalgae, it...
Pinnatoxin G (PnTx-G) is a marine cyclic imine toxin produced by the dinoflagellate Vulcanodinium rugosum, frequently detected in edible shellfish from Ingril Lagoon (France). As other pinnatoxins, to date, no human poisonings ascribed consumption of PnTx-G contaminated seafood have been reported, despite its potent antagonism at nicotinic acetylcholine receptors and high fast-acting toxicity after intraperitoneal or oral administration mice. The hazard characterization exposure limited...
Various reducing agents and reaction times are systematically investigated to enhance the performance of chemically reduced GO (CrGO) in electronics electrochemical applications.