A5078643842- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Species Distribution and Climate Change
- RNA and protein synthesis mechanisms
- Viral gastroenteritis research and epidemiology
- Plant Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Gastrointestinal motility and disorders
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Advanced Statistical Process Monitoring
- CRISPR and Genetic Engineering
- Environmental DNA in Biodiversity Studies
- Genetics, Aging, and Longevity in Model Organisms
- Legionella and Acanthamoeba research
- Spaceflight effects on biology
- Probiotics and Fermented Foods
- Computability, Logic, AI Algorithms
- Respiratory viral infections research
- Infant Health and Development
- Isotope Analysis in Ecology
- Pharmaceutical studies and practices
- Biomedical and Engineering Education
- Pleistocene-Era Hominins and Archaeology
- Evolution and Paleontology Studies
APC Microbiome Institute
2022-2024
University College Cork
2022-2024
University of California, Merced
2019-2021
University of Leicester
2018-2019
Helmholtz Zentrum München
2019
Royal Brompton Hospital
2019
Imperial College London
2019
Ludwig-Maximilians-Universität München
2019
German Center for Lung Research
2019
University of Glasgow
2018
The microbiota-gut-brain axis has been shown to play an important role in the stress response, but previous work focused primarily on of bacteriome. gut virome constitutes a major portion microbiome, with bacteriophages having potential remodel bacteriome structure and activity. Here we use mouse model chronic social stress, employ 16S rRNA whole metagenomic sequencing faecal pellets determine how is modulated by contributes effects stress. We found that led behavioural, immune alterations...
Social anxiety disorder (SAD) is a crippling psychiatric characterized by intense fear or in social situations and their avoidance. However, the underlying biology of SAD unclear better treatments are needed. Recently, gut microbiota has emerged as key regulator both brain behaviour, especially those related to function. Moreover, increasing data supports role for immune function oxytocin signalling responses. To investigate whether plays causal modulating behaviours relevant SAD, we...
Hypermutable loci are widespread in bacteria as mechanisms for rapid generation of phenotypic diversity within a population that enables survival fluctuating, often antagonistic, selection pressures. Localised hypermutation can mediate phase variation and enable bacteriophage predation due to high frequency, reversible alterations the expression phage receptors. As also generate population-to-population heterogeneity, we hypothesized this phenomenon may facilitate spatially-separated...
Abstract Large-scale metagenomic and data mining efforts have uncovered an expansive diversity of bacteriophages (phages) within the human gut 1–3 . These insights include broader phage populational dynamics such as temporal stability 4 , interindividual uniqueness 5,6 potential associations to specific disease states 7,8 However, functional understanding phage-host interactions their impacts this complex ecosystem been limited due a lack cultured isolates for experimental validation. Here...
In recent years, our understanding of the importance microorganisms on and within bodies has been revolutionized by ability to characterize entire microbial communities. No more so is this true than in cases disease. Community studies have revealed strong associations between populations disease states where such concomitance was previously absent from aetiology: including cancers. The study viruses, particular, benefited development new community profiling techniques we are now realising...
A wealth of viral data sits untapped in publicly available metagenomic sets when it might be extracted to create a usable index for the virological research community. We hypothesized that work this complexity and scale could done hackathon setting. Ten teams comprised over 40 participants from six countries, assembled crowd-sourced set analysis processing pipelines complex biological three-day event on San Diego State University campus starting 9 January 2019. Prior hackathon, 141,676...
Abstract Summary Bacteriophages that have integrated their genomes into bacterial chromosomes, termed prophages, are widespread across bacteria. Prophages key components of genomes, with integration often contributing novel, beneficial, characteristics to the infected host. Likewise, induction—through production and release progeny virions surrounding environment—can considerable ramifications on communities. Yet, not all prophages can excise following integration, due genetic degradation by...
A fundamental assumption of hypothesis-driven decay experiments is that, during decay, the loss anatomy follows a sequence broadly controlled by intrinsic compositional properties tissues. Recent work investigating succession postmortem endogenous microbial communities (thanatomicrobiome) challenges this assumption. These studies suggest thanatomicrobiome exhibits predictable, clock-like in response to physical and chemical environmental changes within carcass. Therefore, it possible that...
Here, we report the 3,426,844-bp draft genome sequence of Legionella pneumophila subsp. strain DSM 25199, a serogroup 1 L. pneumophila. The assembly consists 24 contigs with an N50 300,843 bp.
de niveau recherche, publiés ou non, émanant des établissements d'enseignement et recherche français étrangers, laboratoires publics privés.
Abstract Hypermutable loci are widespread in bacteria as mechanisms for rapid generation of phenotypic diversity, enabling individual populations to survive fluctuating, often antagonistic, selection pressures. As observed adaptive immunity, hypermutation may facilitate survival multiple, spatially-separated bacterial populations. We developed an ‘oscillating prey assay’ examine bacteriophage (phage) spread through Haemophilus influenzae whose phage receptor gene, lic2A , is switched ‘ON’...
Jejunal fluid absorption in vivo was reduced by distension and hydrostatic pressure further declined on adding E. coli STa enterotoxin but no net secretion detected. Luminal atropine mediated reductions to normal values intravenous hexamethonium without effect. A neural component inhibition of (though not stretch) may be axon reflexes within cholinergic neurons.Perfusion compounds also did cause secretion. In order show that these actions were secretory processes stimulated offset rates...
Using a recirculation procedure to perfuse anaesthetised rat jejunum, E. coli STa enterotoxin can be shown inhibit net fluid absorption profoundly, while not causing secretion, provided measurement is by mass or volume. This observation contradicts many reports of implying that the recovered volume technique in animal over period some hours cannot detect secretion because conjectured unspecified flaws. Experiments are presented here confirming viability perfusion protocol used this...