A5060877193- Microtubule and mitosis dynamics
- Circadian rhythm and melatonin
- Genetics, Aging, and Longevity in Model Organisms
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Photoreceptor and optogenetics research
- Cancer-related molecular mechanisms research
- Polydiacetylene-based materials and applications
- Circular RNAs in diseases
- Hippo pathway signaling and YAP/TAZ
- Virus-based gene therapy research
- Prostate Cancer Treatment and Research
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Bacterial Infections and Vaccines
- Radiation Therapy and Dosimetry
- Erythrocyte Function and Pathophysiology
- Supramolecular Self-Assembly in Materials
- Cardiovascular Effects of Exercise
- Nanopore and Nanochannel Transport Studies
- COVID-19 and healthcare impacts
- RNA Research and Splicing
- Molecular Sensors and Ion Detection
- Spaceflight effects on biology
- Microfluidic and Bio-sensing Technologies
Icahn School of Medicine at Mount Sinai
2018-2024
Mount Sinai Hospital
2024
IBM Research - Thomas J. Watson Research Center
2019-2021
Southern Illinois University Carbondale
2012-2019
IBM (United States)
2018-2019
Alliance for Safe Kids
2018
Yale University
2016
Adeno-associated virus (AAV) has emerged as one of the best tools for cardiac gene delivery due to its cardiotropism, long-term expression, and safety. However, a significant challenge successful clinical use is preexisting neutralizing antibodies (NAbs), which bind free AAVs, prevent efficient transduction, reduce or negate therapeutic effects. Here we describe extracellular vesicle-encapsulated AAVs (EV-AAVs), secreted naturally by AAV-producing cells, superior vector that delivers more...
Extracellular vesicles (EVs) offer many opportunities in early-stage disease diagnosis, treatment monitoring, and precision therapy owing to their high abundance bodily fluids, accessibility from liquid biopsy, presence of nucleic acid protein cargo cell origin. Despite growing promise, isolation EVs for analysis remains a labor-intensive time-consuming challenge given nanoscale dimensions (30-200 nm) low buoyant density. Here, we report simple, size-based EV separation technology that...
MicroRNAs (miRNAs) regulate gene expression by post-transcriptional inhibition of target genes. Proangiogenic small extracellular vesicles (sEVs; popularly identified with the name "exosomes") a composite cargo miRNAs are secreted cultured stem cells and present in human biological fluids. Lipid nanoparticles (LNPs) represent an advanced platform for clinically approved delivery RNA therapeutics. In this study, we aimed to (1) identify responsible sEV-induced angiogenesis; (2) develop...
Surveillance tools for early cancer detection are suboptimal, including hepatocellular carcinoma (HCC), and biomarkers urgently needed. Extracellular vesicles (EVs) have gained increasing scientific interest due to their involvement in tumour initiation metastasis; however, most extracellular RNA (exRNA) blood-based biomarker studies limited annotated genomic regions.
Abstract Mycobacterium tuberculosis ( Mtb ) secretes extracellular vesicles (EVs) containing a variety of proteins, lipoproteins, and lipoglycans. While emerging evidence suggests that EVs contribute to pathogenesis, the factors molecular mechanisms involved in mycobacterial EV production have not been identified. In this study, we use genetic approach identify proteins mediate vesicle release response iron limitation antibiotic exposure. We uncover critical role for isoniazid‐induced,...
From eukaryotes to prokaryotes, all cells secrete extracellular vesicles (EVs) as part of their regular homeostasis, intercellular communication, and cargo disposal. Accumulating evidence suggests that small EVs carry functional RNAs, potentially serving messengers liquid-biopsy markers. Yet, the complete transcriptomic landscape EV-associated RNAs during disease progression is poorly delineated due critical limitations including protocols used for sequencing, suboptimal alignment short...
The ultimate goal of this study was developing antimicrobial food-contact materials based on natural phenolic compounds using nanotechnological approaches. Among the methyl-β-cyclodextrin-encapsulated phenolics tested, curcumin showed by far highest activity toward Escherichia coli with a minimum inhibitory concentration 0.4 mM. Curcumin enclosed in liposome-type polydiacetylene/phosholipid nanovesicles supplemented N-hydroxysuccinimide and glucose. fluorescence spectrum suggested that...
We investigate interactions between receptors and ligands at bilayer surface of polydiacetylene (PDA) liposomal nanoparticles using changes in electronic absorption spectroscopy fluorescence resonance energy transfer (FRET). study the effect mode linkage (covalent versus noncovalent) receptor liposome bilayer. also examine size-dependent analyte through FRET responses. Glucose (receptor) molecules were either covalently or noncovalently attached nanoparticles, they provided selectivity for...
Abstract Biological micro-motors (microorganisms) have potential applications in energy utilization and nanotechnology. However, harnessing the power generated by such motors to execute desired work is extremely difficult. Here, we employ of motile bacteria transport small, large giant unilamellar vesicles (SUVs, LUVs GUVs). Furthermore, demonstrate bacteria–bilayer interactions probing glycolipids inside model membrane scaffold. Fluorescence Resonance Energy Transfer (FRET) spectroscopic...
Abstract It is known that small extracellular vesicles (sEVs) are released from cancer cells and contribute to progression via crosstalk with recipient cells. We have previously reported sEVs expressing the αVβ3 integrin, a protein upregulated in aggressive neuroendocrine prostate (NEPrCa), differentiation (NED) Here, we examine impact of expression on sEV content, density function. used this study were isolated by iodixanol gradients characterized nanoparticle tracking analysis,...
Abstract Background The protein encoded by GmRLK18-1 ( Glyma_18_02680 on chromosome 18) was a receptor like kinase (RLK) within the soybean (Glycine max L. Merr.) Rhg1/Rfs2 locus. locus underlies resistance to cyst nematode (SCN) Heterodera glycines (I.) and causal agent of sudden death syndrome (SDS) Fusarium virguliforme (Aoki). Previously leucine rich repeat (LRR) domain expressed in Escherichia coli . Results aims here were evaluate LRRs ability to; homo-dimerize; bind larger proteins;...
This Research Article describes a novel method for removal of particulate contamination, loosely referred to as dust, from solid surfaces using polymeric micropillars. In this Article, we illustrate the first time that microfibrils controlled interfacial and geometrical properties can effectively remove micrometric submicrometric contaminant particles surface without damaging underlying substrate. Once these are brought into contact with contaminated surface, because their soft flexible...
Abstract Understanding the mechanisms underlying resistance is critical to improving therapeutic outcomes in patients with metastatic castration–resistant prostate cancer. Previous work showed that dynamic interconversions between epithelial–mesenchymal transition mesenchymal–epithelial defines phenotypic landscape of tumors, as a potential driver emergence resistance. In this study, we use vitro and vivo preclinical MDA PCa patient-derived xenograft models resistant human cancer determine...
Background: More than 50% of deaths in advanced chronic kidney disease (CKD) are attributed to cardiovascular (CVD). The degree heart failure (HF) correlates with severity CKD. Clinical observations suggest that kidney-derived factors contribute the development HF CKD, independent known co-morbidities such as hypertension and hyperglycemia. However, date, no kidney-specific risk factor triggers early damage has been identified, primarily due a paucity studies patients reno-cardiac disease....