A5100691147- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Microfluidic and Bio-sensing Technologies
- Pharmacological Effects of Natural Compounds
- Innovative Microfluidic and Catalytic Techniques Innovation
- Video Analysis and Summarization
- Cell Image Analysis Techniques
- Lung Cancer Treatments and Mutations
- Technology and Data Analysis
- Image Retrieval and Classification Techniques
- Medical Imaging Techniques and Applications
- Analytical Chemistry and Chromatography
- Advanced DC-DC Converters
- Barrier Structure and Function Studies
- Cellular Mechanics and Interactions
- Food Quality and Safety Studies
- Computational Drug Discovery Methods
- Drug Solubulity and Delivery Systems
- Embedded Systems and FPGA Design
- Parallel Computing and Optimization Techniques
- Plant-based Medicinal Research
- CAR-T cell therapy research
- HER2/EGFR in Cancer Research
- Phytochemistry and Bioactivity Studies
- Advanced Sensor and Control Systems
Gachon University
2022-2024
Bio-Medical Science (South Korea)
2018-2024
Yonsei University
2004-2024
Electronics and Telecommunications Research Institute
2006-2023
University of Chinese Academy of Sciences
2023
Sejong University
2023
Inje University
2023
PeopleBio (South Korea)
2022
Samsung (South Korea)
2004-2021
Sungkyunkwan University
2004-2021
Background: Particulate matter (PM) can penetrate deep into the lungs, leading to diseases through induction of hypoxia and inflammation. The hypoxia-inducible factor-1α (HIF-1α) protein is well-established as a key factor in hypoxic response, PM exposure has been linked induction. To develop model suitable for studying PM-induced hypoxia, we compared expression HIF-1α between conventional 2-dimensional (2D) cell culture 3-dimensional (3D) model.Methods: BEAS-2B cells were cultured 2D (using...
Noble Gemini surfactants containing a siloxane moiety have been designed and successfully synthesized in the present study are utilized as structure-directing agents for mesoporous metal oxides such zirconia, titania, vanadia. The is believed to play an important nano-propping role during surfactant removal by direct calcination, yielding thermally stable oxides. It also that synthesis strategy described here can be applied of robust nanostructured materials nanoparticles nanorods addition materials.
We investigated the neuritogenic effects of Tremella fuciformis (TF), which has been valued in traditional Chinese medicine as a remedy with nutritive and tonic actions, on PC12h cells. The cognitive improving TF scopolamine-induced (2 mg/kg, s.c.) amnesia rats were also evaluated using Morris water maze task by performing choline acetyltransferase (ChAT) immunohistochemistry. extract (0.01-1 microg/ml) promoted neurite outgrowth cells dose dependent manner. was highly efficient at...
Glioblastoma (GBM) is classified as one of the most aggressive and lethal brain tumor. Great strides have been made in understanding genomic molecular underpinnings GBM, which translated into development new therapeutic approaches to combat such deadly disease. However, there are only few agents that can effectively inhibit GBM invasion a clinical framework. In an effort address challenges, we generated anti-SEMA3A monoclonal antibody potential against progression.We employed public glioma...
A common method of three-dimensional (3D) cell cultures is embedding single cells in Matrigel. Separated Matrigel migrate or grow to form spheroids but lack cell-to-cell interaction, which causes difficulty delay forming mature spheroids. To address this issue, we proposed a 3D aggregated spheroid model (ASM) create large by aggregating attached the surface 96-pillar plates. Before gelling Matrigel, placed pillar inserts into blank wells where gravity allowed gather at curved end. In drug...
Recently, cancer organoid-based drug sensitivity tests have been studied to predict patient responses anticancer drugs. The area under curve (AUC) or IC50 value of the dose-response (DRC) is used differentiate between sensitive and resistant patient's groups. This study proposes a multi-parameter analysis method (cancer diagnosis reactivity prediction, CODRP) that considers stage cell growth rate, which represent severity patients, in test.On CODRP platform, patient-derived organoids (PDOs)...
Plant extract fermentation is widely employed to enhance the nutritional and pharmaceutical value of functional foods. Polygonum cuspidatum (Pc) contains flavonoids, anthraquinones, stilbenes, imparting protective effects against inflammatory diseases, cancer, diabetes, cardiovascular diseases. However, fermented Pc on skeletal muscle strength remain unexplored. In this study, we generated using a complex microorganisms containing Lactobacillus spp. (McPc) assessed its motor function in...
Hypoxia is a representative tumor characteristic associated with malignant progression in clinical patients. Engineered vitro models have led to significant advances cancer research, allowing for the investigation of cells physiological environments and study disease mechanisms processes enhanced relevance. In this study, we propose U-shape pillar strip 3D cell-lumped organoid model (3D-COM) effects hypoxia on lung high-throughput manner. We developed U-pillar that facilitates aggregation...
ABSTRACT Shiga toxin 1 (Stx1) transiently increases the expression of proinflammatory cytokines by macrophage-like THP-1 cells in vitro. Increased cytokine production is partly due to activation translation initiation factor eIF4E through a mitogen-activated protein kinase (MAPK)- and Mnk1-dependent pathway. availability for regulated association with binding proteins (4E-BP). In this study, we showed that Stx1 induced 4E-BP hyperphosphorylation, which may release initiation. Phosphorylation...
A micropillar/microwell chip platform was applied to develop a 3D cell-based high-content screening (HCS) platform. Previously, cell culture in the only limited viability measurements high-throughput manner. However, an HCS system could provide biological and phenotypic information which very useful understand complex functions mechanisms of drug actions. To stain cultured cells immobilized with alginate spots, we developed optimized antibody staining procedures for cells. As proof concept,...
Novel RGB-LED driving system for LED backlight in LCD display have been developed and demonstrated. The consists of the non-isolation type high efficiency constant current DC-DC converter dynamic controller with microcontroller. driver is up to 94.4% maximum color deviation, Δu'v', from target 0.002.
A higher correlation of epidermal growth factor receptor (EGFR)-targeting drugs has been reported with the use cell proliferation receptor-enhanced three-dimensional high-throughput screening model (CPRE 3D-HTS model) compared two-dimensional (2D) cell-based HTS. greater expression differential human EGFR 2 (HER2) protein between HER2-positive and HER2-negative lines was observed in breast cancer (BC) cultured using CPRE 2D-cultured cells. When cells, properties such as are lost cells attach...
Abstract Various three-dimensional (3D) cell culture methods have been developed to implement tumor models similar in vivo. However, the conventional 3D method has limitations such as difficulty using an extracellular matrix (ECM), low experimental reproducibility, complex protocol, and applying high array plates 96- or 384-plates. Therefore, detailed protocols related robust 3D-aggregated spheroid model (3D-ASM) production were optimized proposed. A specially designed wet chamber was used...
Roasting has been widely used in manufacturing food products, ensuring microbial safety, and improving extraction efficiency. treatment affects various qualities such as the chemical composition, physical properties, colour, aroma, texture of food. This study determined effect novel convection heated roasting on aroma taste components, that is, amino acids (AAs) nucleotides vegetables, meat, their products. Heat conditions were confirmed with a temperature condition 250°C for 20–50 min each...
This study proposed an optimized histogel construction method for histological analysis by applying lung cancer patient-derived organoids (PDOs) to the developed histo-pillar strip. Previously, there is cultured PDOs damage problem during due forced detachment of Matrigel spots from 96-well plate bottom. To address this issue, we PDO on Histo-pillar strips and then immersed them in 4% paraformaldehyde fixation solution self-isolate without damage. The 4
A high throughput apoptosis assay using 3D cultured cells was developed with a micropillar/microwell chip platform. Live cell assays based on fluorescence detection have been useful in content screening. To check the autofluorescence of drugs, controls (no caspase-3/7 reagent assay) for drugs are necessary which require twice test space. Thus, capability and highly miniaturized format reducing usage live assays. Especially, expensive should be reduced screening system. solve this issue, we...