A5100649594- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Cancer Cells and Metastasis
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Capillary Electrophoresis Applications
- Cell Image Analysis Techniques
- Immunotherapy and Immune Responses
- Advanced Biosensing Techniques and Applications
- HER2/EGFR in Cancer Research
- Peptidase Inhibition and Analysis
- Conducting polymers and applications
- Advanced Sensor and Energy Harvesting Materials
- Computational Drug Discovery Methods
- Viral Infectious Diseases and Gene Expression in Insects
- Lung Cancer Treatments and Mutations
- Cellular Mechanics and Interactions
- Cancer Genomics and Diagnostics
- Protein Degradation and Inhibitors
- Electrowetting and Microfluidic Technologies
- Composite Structure Analysis and Optimization
- Monoclonal and Polyclonal Antibodies Research
- Biosensors and Analytical Detection
- Analytical Chemistry and Sensors
- Advanced Machining and Optimization Techniques
- Transportation Safety and Impact Analysis
Seoul National University
2023-2025
Gachon University
2022-2024
Bio-Medical Science (South Korea)
2021-2023
Inha University
2018-2022
Konyang University
2017-2022
Sungkyunkwan University
2017-2021
Korea Research Institute of Standards and Science
2021
Samsung (South Korea)
2012-2016
Cleveland State University
2016
Yeungnam University
2011-2014
Pancreatic ductal adenocarcinoma (PDAC) is a stroma-rich carcinoma, and pancreatic stellate cells (PSCs) are major component of this dense stroma. PSCs play significant roles in metastatic progression chemoresistance through cross-talk with cancer cells. Preclinical vitro tumor model invasive phenotype should incorporate three-dimensional (3D) culture extracellular matrix (ECM) for clinical relevance predictability.PANC-1 were cultured as spheroids (TSs) using our previously developed...
Abstract Immunotherapy has emerged as a promising approach to treating several forms of cancer. Use immune cells, such natural killer (NK) along with small molecule drugs and antibodies through antibody dependent cell-mediated cytotoxicity (ADCC) been investigated potential combination therapy for some difficult treat solid tumors. Nevertheless, there remains need develop tools that support co-culture target cancer cells effector in contextually relevant three-dimensional (3D) environment...
The efficiency of volatile formaldehyde removal was assessed in 86 species plants representing five general classes (ferns, woody foliage plants, herbaceous Korean native and herbs). Phytoremediation potential by exposing the to gaseous (2.0 μL·L −1 ) airtight chambers (1.0 m 3 constructed inert materials measuring rate removal. Osmunda japonica , Selaginella tamariscina Davallia mariesii Polypodium formosanum Psidium guajava Lavandula spp., Pteris dispar multifida Pelargonium spp. were most...
Background: Particulate matter (PM) can penetrate deep into the lungs, leading to diseases through induction of hypoxia and inflammation. The hypoxia-inducible factor-1α (HIF-1α) protein is well-established as a key factor in hypoxic response, PM exposure has been linked induction. To develop model suitable for studying PM-induced hypoxia, we compared expression HIF-1α between conventional 2-dimensional (2D) cell culture 3-dimensional (3D) model.Methods: BEAS-2B cells were cultured 2D (using...
This study aimed to evaluate the osteogenic capacity of two types collagenated xenogenic bone grafts (OCS-B Collagen®, NIBEC, Jincheon, Korea and Bio-Oss® Collagen, Geistlich, Wolhusen, Switzerland) in artificial defects beagle dogs. The bilateral mandibular premolars 13 male beagles were extracted, followed by creation standardized bony after 4 weeks. grafted with or without covered a collagen membrane. Dental implants placed 24 weeks post grafting. Bone regeneration osseointegration...
The removal of Solvent Yellow 1 in a range from 0.25 to 10 ppm by weight an aqueous solution using predispersed solvent extraction (PDSE) was investigated and compared with conventional extractions batch mode. Colloidal liquid aphrons (CLAs) made out kerosene were used as organic solvent. To form CLAs, Tergitol 15-S-3 sodium dodecylbenzene sulfonate oil-soluble water-soluble surfactants, respectively. ratio volume pregnant less than 0.025. efficiency the CLAs can be higher that single-staged...
A common method of three-dimensional (3D) cell cultures is embedding single cells in Matrigel. Separated Matrigel migrate or grow to form spheroids but lack cell-to-cell interaction, which causes difficulty delay forming mature spheroids. To address this issue, we proposed a 3D aggregated spheroid model (ASM) create large by aggregating attached the surface 96-pillar plates. Before gelling Matrigel, placed pillar inserts into blank wells where gravity allowed gather at curved end. In drug...
Recently, cancer organoid-based drug sensitivity tests have been studied to predict patient responses anticancer drugs. The area under curve (AUC) or IC50 value of the dose-response (DRC) is used differentiate between sensitive and resistant patient's groups. This study proposes a multi-parameter analysis method (cancer diagnosis reactivity prediction, CODRP) that considers stage cell growth rate, which represent severity patients, in test.On CODRP platform, patient-derived organoids (PDOs)...
Hypoxia is a representative tumor characteristic associated with malignant progression in clinical patients. Engineered vitro models have led to significant advances cancer research, allowing for the investigation of cells physiological environments and study disease mechanisms processes enhanced relevance. In this study, we propose U-shape pillar strip 3D cell-lumped organoid model (3D-COM) effects hypoxia on lung high-throughput manner. We developed U-pillar that facilitates aggregation...
A micropillar/microwell chip platform was applied to develop a 3D cell-based high-content screening (HCS) platform. Previously, cell culture in the only limited viability measurements high-throughput manner. However, an HCS system could provide biological and phenotypic information which very useful understand complex functions mechanisms of drug actions. To stain cultured cells immobilized with alginate spots, we developed optimized antibody staining procedures for cells. As proof concept,...
In operando techniques have emerged to elucidate the fundamental mechanism of triboelectrification via relevant physical quantity measurement during contact two materials. Here, a correlation between frictional heat and triboelectric charge is reported in metal-polymer nanogenerator through temperature measurement. Fluorine-doped tin oxide (FTO) metal slid back-and-forth under different pressures across polydimethylsiloxane (PDMS) polymers having degrees crosslinking, i.e., mixing ratio....
Three-dimensional (3D) culture platforms have been adopted in a high-throughput screening (HTS) system to mimic vivo physiological microenvironments. The automated dispenser has established commercially enable spotting or distributing non-viscous viscous biomaterials onto microplates. However, there are still challenges the precise and accurate dispensation of cells embedded hydrogels such as Alginate- Matrigel-extracellular matrices. We developed improved an contact-free dispensing machine,...
We propose high-radix microfluidic multiplexers that effectively address a large number of flow channels with few control lines having pressure valves different thresholds. Previous binary only two by controlling identical threshold in lines; is, they 2(n/2) using n lines. However, the proposed ternary or quaternary multiplexer (high-radix multiplexers) addresses three four kinds lines, respectively. Thus, these 3(n/2) and 4(n/2) channels, which are results increasing radix from to...
A higher correlation of epidermal growth factor receptor (EGFR)-targeting drugs has been reported with the use cell proliferation receptor-enhanced three-dimensional high-throughput screening model (CPRE 3D-HTS model) compared two-dimensional (2D) cell-based HTS. greater expression differential human EGFR 2 (HER2) protein between HER2-positive and HER2-negative lines was observed in breast cancer (BC) cultured using CPRE 2D-cultured cells. When cells, properties such as are lost cells attach...
Proton beam therapy (PBT) is a critical treatment modality for head and neck squamous cell carcinoma (HNSCC). However, not much known about drug combinations that may improve the efficacy of PBT. This study aimed to test feasibility three-dimensional (3D) tumor-spheroid-based high-throughput screening platform could assess cellular sensitivity against Spheroids two HNSCC lines—Fadu Cal27—cultured with mixture Matrigel were arrayed on 384-pillar/well plate, followed by exposure graded doses...
Abstract Various three-dimensional (3D) cell culture methods have been developed to implement tumor models similar in vivo. However, the conventional 3D method has limitations such as difficulty using an extracellular matrix (ECM), low experimental reproducibility, complex protocol, and applying high array plates 96- or 384-plates. Therefore, detailed protocols related robust 3D-aggregated spheroid model (3D-ASM) production were optimized proposed. A specially designed wet chamber was used...