A5081013984- Trypanosoma species research and implications
- Primate Behavior and Ecology
- Photoreceptor and optogenetics research
- 3D Printing in Biomedical Research
- Neurobiology and Insect Physiology Research
- Anesthesia and Sedative Agents
- Additive Manufacturing and 3D Printing Technologies
- Anesthesia and Neurotoxicity Research
- Neural dynamics and brain function
- Thermal Regulation in Medicine
- Genetic Mapping and Diversity in Plants and Animals
- Lysosomal Storage Disorders Research
- Research on Leishmaniasis Studies
- Microfluidic and Bio-sensing Technologies
- Multisensory perception and integration
- Nanofabrication and Lithography Techniques
- Insect symbiosis and bacterial influences
- Single-cell and spatial transcriptomics
- Gene Regulatory Network Analysis
- Neuroendocrine regulation and behavior
- Signaling Pathways in Disease
- Intensive Care Unit Cognitive Disorders
The Francis Crick Institute
2022-2024
Institut Pasteur
2018-2020
Our perception of the world depends on brain's ability to integrate information from multiple senses, with temporal disparities providing a critical cue for binding or segregating cross-modal signals. The superior colliculus (SC) is key site integrating sensory modalities, but how cellular and network mechanisms in distinct anatomical regions within SC contribute multisensory integration remains poorly understood. Here, we recorded responses over 5,000 neurons across SC's axes awake mice...
Presenting visual stimuli in neuroscience experiments often requires the combination of analogue signals that carry information about cue presented on LCD display. Such are sensed by photodetectors and recorded to digital converter (ADC) acquisition boards. The use open-source programming languages such as Bonsai is becoming more popular. They used with other hardware Arduino development These microcontroller-based boards can be automate behavioural experiments: e.g., actuate valves motors...
Preclinical studies of psychiatric disorders require the use animal models to investigate impact environmental factors or genetic mutations on complex traits such as decision-making and social interactions. Here, we present a real-time method for behavior analysis mice housed in groups that couples computer vision, machine learning Triggered-RFID identification track monitor animals over several days enriched environments. The system extracts thorough list individual collective behavioral...
Cell culture devices, such as microwells and microfluidic chips, are designed to increase the complexity of cell-based models while retaining control over conditions have become indispensable platforms for biological systems modelling. From microtopography, microwells, plating larger constructs live imaging chamber slides, a wide variety devices with different geometries in biology laboratories. However, their application projects is increasing exponentially, due combination techniques,...
Abstract Background Trypanosoma brucei exhibits a complex life-cycle alternating between tsetse flies and mammalian hosts. When parasites infect the fly, cells differentiate to adapt life in various tissues, which is accompanied by drastic morphological biochemical modifications especially proventriculus. This key step represents bottleneck for salivary gland infection. Methods Here, we monitored flagellum assembly trypanosomes during differentiation from trypomastigote epimastigote stage,...
Behaviour is the ultimate output of neural circuit computations, and therefore its analysis a cornerstone neuroscience research. However, every animal experimental paradigm requires different illumination conditions to capture and, in some cases, manipulate specific behavioural features. This means that researchers often develop, from scratch, their own solutions set-ups. Here, we present OptoPi, an open source, affordable (∼ £600), arena with accompanying multi-animal tracking software. The...
Abstract Cell culture devices, such as microwells and microfluidic chips, are designed to increase the complexity of cell-based models whilst retaining control over conditions have become indispensable platforms for biological systems modelling. From microtopography, microwells, plating devices larger constructs specific applications live imaging chamber slides, a wide variety with different geometries in biology laboratories. However, while their application projects is increasing...
Abstract Behaviour is the ultimate output of neural circuit computations, and therefore its analysis a cornerstone neuroscience research. However, every animal experimental paradigm requires different illumination conditions to capture and, in some cases, manipulate specific behavioural features. This means that researchers often develop, from scratch, their own solutions set-ups. Here, we present OptoPi, an open source, affordable (∼ £600), arena with accompanying multi-animal tracking...
Abstract Trypanosoma brucei exhibits a complex life cycle alternating between tsetse flies and mammalian hosts. When parasites infect the fly, cells differentiate to adapt in various tissues, which is accompanied by drastic morphological biochemical modifications especially proventriculus. This key step represents bottleneck for salivary gland infection. Here we monitored flagellum assembly trypanosomes during differentiation from trypomastigote epimastigote stage, i.e. when nucleus migrates...
Abstract Studying the development of neural circuits in rodent models requires surgical access to neonatal brain. Since commercially available stereotaxic and anesthetic equipment is designed for use adults, reliable targeting brain structures such young animals can be challenging. Hypothermic cooling (cryoanesthesia) has been used as a preferred anesthesia approach neonates. This commonly involves submerging neonates ice, an that poorly controllable. We have developed affordable, simple...