A5070770390- Wound Healing and Treatments
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Diagnosis and Treatment of Venous Diseases
- Silk-based biomaterials and applications
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- RNA regulation and disease
- Culinary Culture and Tourism
- Mesenchymal stem cell research
- Extracellular vesicles in disease
- Electrospun Nanofibers in Biomedical Applications
- Pancreatic and Hepatic Oncology Research
- Pregnancy-related medical research
- MicroRNA in disease regulation
- Pressure Ulcer Prevention and Management
- Immune Response and Inflammation
- Tannin, Tannase and Anticancer Activities
- CAR-T cell therapy research
- Neuroblastoma Research and Treatments
- Plant and Fungal Interactions Research
- Silkworms and Sericulture Research
- Diamond and Carbon-based Materials Research
Karolinska Institutet
2018-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
Karolinska University Hospital
2024
Peking Union Medical College Hospital
2024
Institute of Dermatology
2024
East China Normal University
2024
Bucharest University of Economic Studies
2016
Venous ulcers are the most common type of human chronic nonhealing wounds and stalled in a constant excessive inflammatory state. The molecular mechanisms underlying wound inflammation remain elusive. Moreover, little is known about role regulatory RNAs, such as microRNAs, pathogenesis venous ulcers. We found that both microRNA (miR)-34a miR-34c were upregulated wound-edge epidermal keratinocytes compared with normal or skin. In keratinocytes, miR-34a promoted chemokine cytokine production....
An increasing number of studies reveal the importance long noncoding RNAs (lncRNAs) in gene expression control underlying many physiological and pathological processes. However, their role skin wound healing remains poorly understood. Our study focused on a skin-specific lncRNA, LOC105372576, whose was increased during healing. In human nonhealing wounds, however, its level significantly lower compared with normal wounds under reepithelialization. We characterized LOC105372576 as...
Chronic wounds represent a major and growing health economic burden worldwide. A better understanding of molecular mechanisms normal as well impaired wound healing is needed to develop effective treatment. Herein we studied the potential role long noncoding RNA LOC100130476 in skin repair. an polymerase II–encoded polyadenylated transcript present both cytoplasm nucleus. We found that its expression was lower wound-edge keratinocytes human chronic compared healthy donors intact skin. In...
Objective: Insufficient knowledge about the molecular pathology of diabetic foot ulcer (DFU) impedes development effective wound treatment. Circular RNAs (circRNAs) are a novel class RNA recently discovered to be widely expressed and have important biological functions; however, their role in skin healing remains largely unexplored. In this study, we investigated circRNAs DFU. Approach: CircRNA expression was profiled normal wounds (NWs) DFUs by microarray analysis, hsa_circ_0084443...
MicroRNAs (miR), as important epigenetic control factors, reportedly regulate wound repair. However, our insufficient knowledge of clinically relevant miRs hinders their potential therapeutic use. For this, we performed paired small and long RNA-sequencing integrative omics analysis in human tissue samples, including matched skin acute wounds collected at each healing stage chronic nonhealing venous ulcers (VUs). On the basis findings, developed a compendium (...
Abstract The cell transition from an inflammatory phase to a subsequent proliferative is crucial for wound healing, yet the driving mechanism remains unclear. By profiling lncRNA expression changes during human skin healing and screening functions, we identify SNHG26 as pivotal regulator in keratinocyte progenitors underpinning this transition. Snhg26 -deficient mice exhibit impaired repair characterized by delayed re-epithelization accompanied exacerbated inflammation. Single-cell...
Pancreatic ductal adenocarcinoma (PDAC) is one of the leading causes cancer-related deaths and novel treatment approaches are urgently needed. Here we show that poly(ethylene glycol)-functionalized nanodiamonds loaded with doxorubicin (ND-PEG-DOX) afforded a considerable improvement over free drug in an orthotopic pancreatic xenograft model. ND-PEG-DOX complexes were also superior to DOX 3-dimensional (3D) tumor spheroids PDAC. ND-PEG showed no cytotoxicity towards macrophages,...
Abstract After a skin injury, keratinocytes switch from state of homeostasis to one regeneration leading the reconstruction epidermal barrier. The regulatory mechanism gene expression underpinning this key during human wound healing is enigmatic. Long noncoding RNAs (lncRNAs) constitute new horizon in understanding programs encoded mammalian genome. By comparing transcriptome an acute and same donor as well isolated these paired tissue samples, we generated list lncRNAs showing changed...
Pressure ulcer (PU) is a chronic wound often seen in patients with spinal cord injury and other bed-bound individuals, particularly the elderly population. Despite its association high mortality, pathophysiology of PU remains poorly understood. In this study, we compared single-cell transcriptomic profiles human epidermal cells from edges those uninjured skin acute wounds healthy donors. We identified significant shifts cell composition gene expression patterns PU. particular, found that...
Venous ulcers (VUs) have complex and obscure pathogenicity, effective VU therapies are still lacking. Circular RNAs (circRNAs) emerged as powerful gene regulators with important roles in health disease. In this study, we used paired total RNA small sequencing to profile circRNAs, protein-coding mRNAs, microRNAs expression a unique collection of clinical samples: healthy skin acute wounds at inflammatory proliferative phases wound-edge biopsies. We unravel dynamically changed pattern circRNAs...
Summary Both circular RNAs (circRNA) and microRNAs (miRNA) have emerged to play important roles in health disease. To understand their function tissue repair, we profiled circRNA, linear RNA, miRNA expression dynamics human wound-edge keratinocytes across the wound healing process. Our investigation spotlighted circASH1L(4,5) its engagement with miR-129-5p, both of which levels were increased repair. Unlike conventional sponging, circASH1L enhanced miR-129 stability silencing activity by...
<title>Abstract</title> The cell transitionfrom an inflammatory phase to a subsequent proliferative is crucial for wound healing, yet the driving mechanism remains unclear. By profiling lncRNA expression changesduring human skin healing and screening functions, we identified<italic>SNHG26</italic> as pivotal regulator in keratinocyte progenitors underpinning this transition. <italic>Snhg26</italic>-deficient mice exhibited impaired repair characterized by delayed re-epithelization...
Skin wound healing involves a complex gene expression program that remains largely undiscovered in humans. Circular RNAs (circRNAs) and microRNAs (miRNAs) are key players this process.
Abstract Pressure ulcer (PU) is a chronic wound often seen in spinal cord injury patients and other bed-bound individuals, particularly the elderly population. Despite its association with high mortality, pathophysiology of PU remains poorly understood. Here, we compared single-cell transcriptomic profiles human epidermal cells from edges those uninjured skin acute wounds (AWs) healthy donors. We identified significant shifts cell composition gene expression patterns PU. In particular, found...
Abstract Background Although the widespread expression of circular RNAs (circRNAs) has only been recognized recently, increasing evidence suggested their important roles in health and disease. To identify clinically relevant circRNAs with potential for wound diagnosis therapy, an in-depth characterization circRNA human healing non-healing wounds is a prerequisite that not attained yet. Methods We collected wound-edge biopsies through process healthy donors chronic venous ulcers (VU). Paired...
ABSTRACT After a skin injury, keratinocytes switch from state of homeostasis to one regeneration leading the reconstruction epidermal barrier. The regulatory mechanism gene expression underpinning this key during human wound healing is enigmatic. Long noncoding RNAs (lncRNAs) constitute new horizon in understanding programmes encoded mammalian genome. By comparing transcriptome an acute and same donor as well isolated these paired tissue samples, we generated list lncRNAs showing changed...
Abstract After a skin injury, keratinocytes switch from state of homeostasis to one regeneration leading the reconstruction epidermal barrier. The regulatory mechanism gene expression underpinning this key during human wound healing is enigmatic. Long noncoding RNAs (lncRNAs) constitute new horizon in understanding programmes encoded mammalian genome. By comparing transcriptome an acute and same donor as well isolated these paired tissue samples, we generated list lncRNAs showing changed...
Abstract Delayed skin wound healing and excessive scarring are consequences of an impaired process represent a major health economic burden worldwide. Current intervention strategies lack efficacy suffer from high recurrence rates necessitating the investigation into alternative treatment modalities like circular RNAs (circRNAs). By RNA sequencing, we profiled circRNA expression changes during human as well in keratinocytes fibroblasts isolated donor-matched acute wounds. CircGLIS3 was found...