A5045535478- Wound Healing and Treatments
- Cancer-related molecular mechanisms research
- Reconstructive Surgery and Microvascular Techniques
- Mesenchymal stem cell research
- Circular RNAs in diseases
- Periodontal Regeneration and Treatments
- Tissue Engineering and Regenerative Medicine
- Trauma Management and Diagnosis
- Hip and Femur Fractures
- LGBTQ Health, Identity, and Policy
- CAR-T cell therapy research
- Single-cell and spatial transcriptomics
- Surgical site infection prevention
- MicroRNA in disease regulation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Effects of Radiation Exposure
- Diagnosis and Treatment of Venous Diseases
- Pressure Ulcer Prevention and Management
- Breast Implant and Reconstruction
- Blood transfusion and management
- 3D Printing in Biomedical Research
- Osteoarthritis Treatment and Mechanisms
- Vascular Procedures and Complications
- Pluripotent Stem Cells Research
- Bone fractures and treatments
Karolinska University Hospital
2016-2025
Karolinska Institutet
2014-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
East China Normal University
2024
Peking Union Medical College Hospital
2024
Institute of Dermatology
2024
University of Miami
2023
Linköping University
2009-2013
The apparent need of an autologous cell source for tissue engineering applications has led researchers to explore the presence cells with stem plasticity in several human tissues. Dermal fibroblasts (FBs) are easy harvest, expand vitro and store, rendering them plausible candidates cell-based therapies. aim present study was observe effects adipogenic, chondrogenic osteogenic induction media on phenotype FBs. Human preadipocytes obtained from fat have been proposed as adult suitable...
BACKGROUND Over the past decades, focus on regenerative properties of platelets (PLTs) has intensified and many PLT‐derived growth factors are readily used in medical settings. A general lack standardization preparation these remains, however, this study therefore examines dynamics throughout freeze‐thaw procedure. STUDY DESIGN AND METHODS Plateletpheresis (PA) PLT‐poor plasma (PPP) samples were collected from 10 healthy donors. PA was lysed to produce PLT lysate (PL) for 1, 3, 5, 10, 30...
Persistent and impaired inflammation impedes tissue healing is a characteristic of chronic wounds. A better understanding the mechanisms controlling wound needed. In this study, we show that in human wound-edge keratinocytes, expressions microRNA (miR)-17, miR-18a, miR-19a, miR-19b, miR-20a, which all belong to miR-17∼92 cluster, are upregulated during repair. However, their levels lower ulcers than acute wounds at proliferative phase. Conditional knockout keratinocytes as well injection...
An increasing number of studies reveal the importance long noncoding RNAs (lncRNAs) in gene expression control underlying many physiological and pathological processes. However, their role skin wound healing remains poorly understood. Our study focused on a skin-specific lncRNA, LOC105372576, whose was increased during healing. In human nonhealing wounds, however, its level significantly lower compared with normal wounds under reepithelialization. We characterized LOC105372576 as...
Abstract The cell transition from an inflammatory phase to a subsequent proliferative is crucial for wound healing, yet the driving mechanism remains unclear. By profiling lncRNA expression changes during human skin healing and screening functions, we identify SNHG26 as pivotal regulator in keratinocyte progenitors underpinning this transition. Snhg26 -deficient mice exhibit impaired repair characterized by delayed re-epithelization accompanied exacerbated inflammation. Single-cell...
MicroRNAs (miR), as important epigenetic control factors, reportedly regulate wound repair. However, our insufficient knowledge of clinically relevant miRs hinders their potential therapeutic use. For this, we performed paired small and long RNA-sequencing integrative omics analysis in human tissue samples, including matched skin acute wounds collected at each healing stage chronic nonhealing venous ulcers (VUs). On the basis findings, developed a compendium (...
Chronic wounds represent a major and growing health economic burden worldwide. A better understanding of molecular mechanisms normal as well impaired wound healing is needed to develop effective treatment. Herein we studied the potential role long noncoding RNA LOC100130476 in skin repair. an polymerase II–encoded polyadenylated transcript present both cytoplasm nucleus. We found that its expression was lower wound-edge keratinocytes human chronic compared healthy donors intact skin. In...
Abstract The discovery of an increasing number translatable circular RNAs (circRNAs) raises the question whether their coding and non‐coding functions can coexist within same cell. This study profiles dynamic expression circRNAs during human skin wound healing. CircGLIS3(2) is identified, a circRNA whose levels transiently rise in dermal fibroblasts acute wounds are abnormally overexpressed keloids, fibrotic condition. Injury signals such as IL‐1α, TGF‐β, hypoxia, ER stress induce both...
Severe trauma to the extremities often includes a combination of fractures and soft tissue injuries. Several publications support that patient outcome is better when skeletal stabilization followed by early soft-tissue coverage. In an effort optimize treatment these patients, we established formalized collaboration in 2008 between Departments reconstructive plastic surgery orthopedics at Karolinska University Hospital.A retrospective review was conducted for all patients who had suffered...
Abstract After a skin injury, keratinocytes switch from state of homeostasis to one regeneration leading the reconstruction epidermal barrier. The regulatory mechanism gene expression underpinning this key during human wound healing is enigmatic. Long noncoding RNAs (lncRNAs) constitute new horizon in understanding programs encoded mammalian genome. By comparing transcriptome an acute and same donor as well isolated these paired tissue samples, we generated list lncRNAs showing changed...
Our aim in this study was to examine which factors are associated with post-operative complications after surgery for pressure ulcers individuals spinal cord injury. We performed a retrospective cohort including all injured patients undergoing ulcer our department between 2002 and 2019. Patient demographics comorbidity were collected from medical records, as treatment data information on within 30 days. Assessment of outcomes through t-tests, χ2-tests ANOVA. Out 118 operations, 51 (43%) had...
Pressure ulcer (PU) is a chronic wound often seen in patients with spinal cord injury and other bed-bound individuals, particularly the elderly population. Despite its association high mortality, pathophysiology of PU remains poorly understood. In this study, we compared single-cell transcriptomic profiles human epidermal cells from edges those uninjured skin acute wounds healthy donors. We identified significant shifts cell composition gene expression patterns PU. particular, found that...
The rates of soft tissue reconstruction and amputation after open tibial fractures have not been studied on a national perspective. We aimed to determine the frequency coverage fracture as well primary secondary rates.Data all patients (> = 15 years) admitted hospital with were extracted from Swedish National Patient Register (1998-2010). All surgical procedures, re-admissions, mechanisms injury analysed accordingly. risk was calculated using logistic regression (adjusted for age, sex,...
Mastectomy and chest-wall contouring is the most common gender confirmation surgery. With increasing prevalence of transgender individuals, there a demand for better surgical outcomes aesthetic results. Our aim was to evaluate techniques used assess modifications in mastectomies at Karolinska University hospital Stockholm, Sweden.A retrospective cohort study performed on 464 patients undergoing our department between 2009 2018. Patient demographics, psychiatric comorbidity, method, outcome...
Abstract Radiotherapy (RT), a common cancer treatment, unintentionally harms surrounding tissues, including the skin, and hinders wound healing years after treatment. This study aims to understand mechanisms behind these late-onset adverse effects. We compare skin biopsies from previously irradiated (RT + ) non-irradiated − sites in breast survivors who underwent RT ago. Here we show that has compromised capacity fibroblast functions. Using ATAC-seq, discover altered chromatin landscapes...
Wound healing is vital for human health, yet the details of cellular dynamics and coordination in wound repair remain largely unexplored. To address this, we conducted single-cell multi-omics analyses on skin tissues through inflammation, proliferation, remodeling phases from same individuals, monitoring molecular at an unprecedented spatiotemporal resolution. This singular roadmap reveals architecture margin identifies FOSL1 as a critical driver re-epithelialization. It shows that...
Background: The impact of preoperative radiotherapy on microvascular reconstructive surgery outcome has been a subject debate. However, data are conflicting and often dependent local treatment protocols. We have studied the effects in unique, single-center setting where protocol change was undertaken from pre- to postoperative administration for microsurgical head neck reconstructions. Methods: A cohort study conducted 200 consecutive free flap cases, 100 were operated before after...
Engineering of bone tissue could help to overcome the need for extensive reconstruction and associated donor site morbidity, it has been proposed that osteogenic biomaterials, which are scaffolds contain osteocompetent cells, be used fill large defects. This study investigated potential osteogenically-induced human dermal fibroblasts cultured on gelatin microcarriers combined with platelet-rich plasma in a model femoral defect athymic rats. Defects were transplanted one following six...
Autologous free tissue transfer is today an integral part of reconstructive plastic surgery, but still lacks generally accepted guidelines regarding antithrombotic agents. We hypothesized that the overuse agents could be a risk factor for flap complications and therefore studied treatment protocol adjustment.Consecutive flaps between 2005 2020 at single center were analyzed in relation to use pre- intraoperative with three different The preoperative low molecular weight heparin (LMWH),...
Venous ulcers (VUs) have complex and obscure pathogenicity, effective VU therapies are still lacking. Circular RNAs (circRNAs) emerged as powerful gene regulators with important roles in health disease. In this study, we used paired total RNA small sequencing to profile circRNAs, protein-coding mRNAs, microRNAs expression a unique collection of clinical samples: healthy skin acute wounds at inflammatory proliferative phases wound-edge biopsies. We unravel dynamically changed pattern circRNAs...
Background: Implant-based breast augmentation is a valuable tool for treatment of gender dysphoria in transgender women. The aim was to assess whether implant attributes, plane selection, and patient characteristics had an impact on the surgical outcome, compare these parameters between cisgender augmentations. Methods: A cohort women who underwent at our department during 2009–2018 were retrospectively studied. also compared with 12,884 mainly registered Swedish registry (BRIMP) 2014–2019....