A5071532772- Diabetes and associated disorders
- Pancreatic function and diabetes
- Systemic Lupus Erythematosus Research
- T-cell and B-cell Immunology
- Atherosclerosis and Cardiovascular Diseases
- MicroRNA in disease regulation
- Diabetes Management and Research
- Rheumatoid Arthritis Research and Therapies
- Birth, Development, and Health
- Systemic Sclerosis and Related Diseases
- Cancer-related molecular mechanisms research
- Monoclonal and Polyclonal Antibodies Research
- Adipose Tissue and Metabolism
- Cytokine Signaling Pathways and Interactions
- Circular RNAs in diseases
- Corneal Surgery and Treatments
- Mosquito-borne diseases and control
- Cardiovascular Disease and Adiposity
- Cytomegalovirus and herpesvirus research
- Psoriasis: Treatment and Pathogenesis
- DNA Repair Mechanisms
- Apelin-related biomedical research
- Prion Diseases and Protein Misfolding
- Vasculitis and related conditions
- Insect symbiosis and bacterial influences
Universidade de São Paulo
2013-2025
Fundação de Amparo à Pesquisa do Estado de São Paulo
2012
National Council for Scientific and Technological Development
2012
Universidade Federal do Rio Grande do Norte
2010
Immungenetics (Germany)
2006-2008
Abstract Background Regardless the regulatory function of microRNAs (miRNA), their differential expression pattern has been used to define miRNA signatures and disclose disease biomarkers. To address question whether patients presenting different types diabetes mellitus could be distinguished on basis mRNA profiling, we obtained peripheral blood mononuclear cell (PBMC) RNAs from 7 type 1 (T1D), 2 (T2D), 6 gestational (GDM) patients, which were hybridized Agilent microarrays. Data...
Aldosterone excess aggravates endothelial dysfunction in diabetes and hypertension by promoting the increased generation of reactive oxygen species, inflammation, insulin resistance. activates molecular platform inflammasome immune system cells contributes to vascular induced mineralocorticoid hormone. It is unclear as whether NLRP3 associated with receptor diabetic conditions. Here, we tested hypothesis that an aldosterone induces type 2 diabetes, via activation receptors (MR) assembly...
Under physiological conditions, the perivascular adipose tissue (PVAT) negatively modulates vascular contractility. This property is lost in experimental and human obesity metabolic syndrome, indicating that changes PVAT function may contribute to dysfunction associated with increased body weight hyperglycemia. The O-linked β-N-acetylglucosamine (O-GlcNAc) modification of proteins (O-GlcNAcylation) a unique posttranslational process integrates glucose metabolism intracellular protein...
Abstract Background Type 1 diabetes (T1D) is an autoimmune disease, while type 2 (T2D) and gestational (GDM) are considered metabolic disturbances. In a previous study evaluating the transcript profiling of peripheral mononuclear blood cells obtained from T1D, T2D GDM patients we showed that gene profile T1D was closer to than T2D. To understand influence demographical, clinical, laboratory, pathogenetic treatment features on profiling, performed analysis integrating these with expression...
Objective. To determine HLA-G expression in skin biopsies from patients with systemic sclerosis (SSc), and its association epidemiological, clinical, laboratory variables survival. Methods. Paraffin-embedded obtained 21 SSc (14 limited SSc, 7 diffuse SSc) 28 healthy controls were studied. was evaluated by immunohistochemistry. Results. molecules detected 57% of (9 3 SSc), whereas no control sample expressed (p = 0.000004). In patients, consistently observed within epidermal some dermal...
Type 1 diabetes (T1D) arises from autoimmune-mediated destruction of insulin-producing β-cells leading to impaired insulin secretion and hyperglycemia. T1D is accompanied by DNA damage, oxidative stress, inflammation, although there still scarce information about the stress response repair in pathogenesis. We used microarray method assess mRNA expression profiles peripheral blood mononuclear cells (PBMCs) 19 patients compared 11 controls identify targets microRNAs that were previously...
This study aimed to evaluate the association between differential gene expression profiling of peripheral blood mononuclear cells rheumatoid arthritis patients with their immunogenetic (human leucocyte antigen shared-epitope, HLA-SE), autoimmune response [anti-cyclic citrullinated peptide (CCP) antibodies], disease activity score (DAS-28) and treatment (disease-modifying antirheumatic drugs tumour necrosis factor blocker) features. Total RNA samples were copied into Cy3-labelled...
Objective. Patients with systemic sclerosis (SSc) exhibit increased toxicity when exposed to genotoxic agents. In our study, we evaluated DNA damage and polymorphic sites in 2 repair genes ( XRCC1 Arg399Gln XRCC4 Ile401Thr) patients SSc. Methods. A total of 177 were studied for gene polymorphisms. Fifty-six them also peripheral blood cells using the comet assay. Results. Compared controls, as a whole or stratified into major clinical variants (limited diffuse skin involvement), irrespective...
Objective. We aimed to evaluate whether the differential gene expression profiles of patients with rheumatoid arthritis (RA) could distinguish responders from nonresponders methotrexate (MTX) and, in case MTX nonresponders, responsiveness plus anti-tumor necrosis factor-α (anti-TNF) combined therapy. Methods. evaluated 25 RA taking 15–20 mg/week as a monotherapy (8 and 17 nonresponders). All received infliximab were reassessed after 20 weeks their anti-TNF using European League Against...
Systemic lupus erythematosus (SLE) is an independent risk factor for cardiovascular events. The present study determined the prevalence of subclinical atherosclerosis in childhood-onset SLE using carotid intima-media thickness (CIMT) measurement and investigated associations between traditional nontraditional factors atherosclerosis, such as medications, Disease Activity Index - SLEDAI-2 K SLICC-ACR damage index CIMT.Cross-sectional prospective 2017 2018. CIMT was assessed by...
The MHC region (6p21) aggregates the major genes that contribute to susceptibility type 1 diabetes (T1D). Three additional relevant regions mapped on chromosomes 1p13 (PTPN22), 2q33 (CTLA‐4), and 11p15 (insulin) have also been described by linkage studies. To evaluate contribution of these house regions, we performed a large‐scale differential gene expression lymphomononuclear cells recently diagnosed T1D patients, pinpointing modulated clustered in their respective chromosomes. A total 4608...
This study aims to evaluate the production of cytokines, tumor necrosis factor (TNF), and interleukin 10 (IL‐10) in peripheral blood mononuclear cells (PBMCs) from type 1 diabetic (T1D) patients by means intracellular staining, flow cytometry, ELISA correlate it with inadequate (IN) adequate (A) metabolic controls. We studied 28 T1D 20 healthy individuals (C) paired sex age. were divided IN A control. PBMC cultures stimulated LPS TNF or PMA/ionomycin concanavalin IL‐10. The levels...
Patients presenting with active systemic lupus erythematosus (SLE) manifestations may exhibit distinct pathogenetic features in relation to inactive SLE. Also, cDNA microarrays potentially discriminate the gene expression profile of a disease or variant. Therefore, we evaluated 4500 genes peripheral blood lymphocytes (PBL) SLE patients. We studied 11 patients (seven and four SLE) eight healthy controls. Total RNA was isolated from PBL, reverse transcribed into cDNA, postlabeled Cy3...