A5072643224- Single-cell and spatial transcriptomics
- Phytochemicals and Medicinal Plants
- Ionosphere and magnetosphere dynamics
- Microfluidic and Bio-sensing Technologies
- Cell Image Analysis Techniques
- Nuclear Engineering Thermal-Hydraulics
- Acute Myeloid Leukemia Research
- Adipokines, Inflammation, and Metabolic Diseases
- Sphingolipid Metabolism and Signaling
- Seismic Waves and Analysis
- Sperm and Testicular Function
- Diabetes and associated disorders
- Risk and Safety Analysis
- Nuclear and radioactivity studies
- Atmospheric Ozone and Climate
- Reproductive Biology and Fertility
- Immune Cell Function and Interaction
- Solar and Space Plasma Dynamics
- melanin and skin pigmentation
- RNA Interference and Gene Delivery
- Astrophysics and Cosmic Phenomena
- Drug Transport and Resistance Mechanisms
- Power Line Inspection Robots
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cancer Cells and Metastasis
East Carolina University
2020-2024
Karolinska University Hospital
2020
Karolinska Institutet
2018-2020
The Netherlands Cancer Institute
2020
Integrated Cardio Metabolic Centre
2018
AstraZeneca (Sweden)
2018
University of Calcutta
2011-2016
Indian Centre for Space Physics
2010-2013
S.N. Bose National Centre for Basic Sciences
2010
Westinghouse Electric (United States)
1997
Colorectal cancer stem cells (CSCs) express Lgr5 and display extensive cell-like multipotency self-renewal are thought to seed metastatic disease. Here, we used a mouse model of colorectal (CRC) human tumor xenografts investigate the cell origin metastases. We found that most disseminated CRC in circulation were Lgr5− formed distant metastases which Lgr5+ CSCs appeared. This plasticity occurred independently stemness-inducing microenvironmental factors was indispensable for outgrowth, but...
Piperine, the main alkaloid of black pepper, Piper nigrum Linn., is an important Indian spice used in traditional food and medicine India. In present study, we investigated antioxidant activities piperine against copper-ascorbate induced toxic injury to mitochondria obtained from a goat heart, vitro. Incubation isolated cardiac with resulted elevated levels lipid peroxidation protein carbonylation mitochondrial membrane, reduced level GSH altered status enzymes as well decreased pyruvate...
Acute myelogenous leukemia (AML), the most prevalent acute and aggressive diagnosed in adults, often recurs as a difficult-to-treat, chemotherapy-resistant disease. Because chemotherapy resistance is major obstacle to successful treatment, novel therapeutic intervention needed. Upregulated ceramide clearance via accelerated hydrolysis glycosylation has been shown be an element AML, problem considering crucial role plays eliciting apoptosis. Herein we employed agents that block determine if...
Abstract. Dual-frequency GPS TEC monitors have been used to study the response of ionosphere solar eclipses 22 July 2009 and 15 January 2010. The receivers were located at three stations, Calcutta, Kharagpur Baharampore which are situated outside umbra zone in Indian longitude sector with each baseline being ~200 km. Effects obscuration disc noted ambient recorded stations. A series depletions along track a satellite associated wave-like structures identified on some links during both eclipses.
ABSTRACT Despite early optimism, therapeutics targeting oxidative phosphorylation (OxPhos) have faced clinical setbacks, stemming from their inability to distinguish healthy cancerous mitochondria. Herein, we describe an actionable bioenergetic mechanism unique mitochondria inside acute myeloid leukemia (AML) cells. Unlike cells which couple respiration the synthesis of ATP, AML were discovered support inner membrane polarization by consuming ATP. Because matrix ATP consumption allows...
Abstract Cytometer characterization is critical to define operational bounds within which the data generated are reliable and reproducible. Existing instrument optimization protocols were developed for cytometers relying on photomultiplier tubes (PMTs) photon detection. Recently, manufacturers have begun incorporating avalanche photodiodes (APDs) in place of PMTs. Differences noise signal amplification properties two detector types make many established PMT inappropriate APD‐based...
In mammalian testes, premeiotic spermatogonia respond to retinoic acid by completing an essential lengthy differentiation program before initiating meiosis. The molecular and cellular changes directing these developmental processes remain largely undefined. This wide gap in knowledge is due two unresolved technical challenges: (1) lack of robust reliable vitro models study meiotic initiation; (2) methods isolate large pure populations male germ cells at each stage initiation. Here, we report...
Melanoma is one of the most aggressive forms cancer, usually resistant to standard chemotherapeutics. Despite a huge number clinical trials, any success find chemotherapeutic agent that can effectively destroy melanoma yet be achieved. Para-phenylenediamine (p-PD) in hair dyes reported purely serve as an external dyeing agent. Very little known about whether p-PD has effect on melanin producing cells. We have demonstrated mediated apoptotic death both human and mouse cells vitro . Mouse...
Abstract During fertilization, mammalian sperm undergo a winnowing selection process that reduces the candidate pool of potential fertilizers from ∼10 6 -10 11 cells to 10 1 2 (depending on species). Classical competition theory addresses positive or ‘stabilizing’ acts phenotypes within populations organisms but does not strictly address developmental consequences traits among individual are under purifying during fertilization. It is latter utmost concern for improving assisted reproductive...
Abstract Compensation or unmixing is essential in analyzing multiparameter flow cytometry data. Errors data correction, either by compensation unmixing, can completely change the outcome mislead researchers. Owing to limited cell numbers, researchers often use synthetic beads generate required single stains for necessary calculation. In this study, capacity of influence correction evaluated. Corrected human peripheral blood cells were generated using cell-based from same bead-based identify...
Abstract In conventional flowcytometry one detector (primary) is dedicated for fluorochrome. However, photons usually end up in other detectors too (fluorescence spillover). ‘Compensation’ a process that corrects the spillover signal from all except primary detector. Post ‘compensation’, photon counting error of signals become evident as spreading data. The induced by impairs ability to resolve stained cell population unstained one, potentially reducing or completely losing populations. For...