A5100637343- Cellular transport and secretion
- Microtubule and mitosis dynamics
- RNA Interference and Gene Delivery
- Wnt/β-catenin signaling in development and cancer
- Cancer Cells and Metastasis
- Glycosylation and Glycoproteins Research
- Hippo pathway signaling and YAP/TAZ
- Biomedical Research and Pathophysiology
- Erythrocyte Function and Pathophysiology
- Advanced biosensing and bioanalysis techniques
- Renal and related cancers
- Venomous Animal Envenomation and Studies
- interferon and immune responses
- HIV-related health complications and treatments
- Lysosomal Storage Disorders Research
- Inflammasome and immune disorders
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- HIV/AIDS drug development and treatment
- melanin and skin pigmentation
- Neonatal and fetal brain pathology
- Renal cell carcinoma treatment
- Advances in Oncology and Radiotherapy
- Fetal and Pediatric Neurological Disorders
- HIV Research and Treatment
China Medical University
2019-2024
Sichuan University
2015-2023
State Key Laboratory of Oral Diseases
2023
State Key Laboratory of Biotherapy
2015-2021
West China Second University Hospital of Sichuan University
2015-2020
Nanchang University
2019
Ningxia Hui Autonomous Region Peoples Hospital
2014-2015
State Key Laboratory of Kidney Diseases
2013
Chinese PLA General Hospital
2013
China Three Gorges University
2010
Retrograde vesicle trafficking pathways are responsible for returning membrane-associated components from endosomes to the Golgi apparatus and endoplasmic reticulum (ER), they critical maintaining organelle identity, lipid homeostasis, many other cellular functions. The retrograde transport pathway has emerged as an important target intravacuolar bacterial pathogens. opportunistic pathogen Legionella pneumophila exploits both secretory recycling branches of intracellular proliferation. Its...
Microtubules (Mts), which consist of α/β-tubulin heterodimers, are involved in cancer development and metastasis. Tubulin cofactor A (TBCA) plays crucial roles modulating tubulin folding heterodimer polymerization. Here, we identified the aberrant expression TBCA clear cell renal carcinoma (ccRCC) specimens as well lines revealed function a novel positive regulator ccRCC progression, invasion qRT-PCR, Western blot immunohistochemistry assays confirmed that was significantly highly expressed...
Abstract The endosomal trafficking pathways are essential for many cellular activities. They also important targets by intracellular pathogens. Key regulators of the include retromer complex and sorting nexins (SNXs). Chlamydia trachomatis effector protein IncE directly components SNX5 SNX6 suppresses retromer-mediated transport, but exact mechanism has remained unclear. We present crystal structure PX domain in with IncE, showing that binds to a highly conserved hydrophobic groove SNX5....
Tyrosinase is a ubiquitous enzyme that plays an essential role in the production of melanin. Effective inhibitors tyrosinase have extensive applications medical, cosmetic and food industries. In this study, combination kinetics, ultraviolet (UV)-visible absorption, fluorescence spectroscopic techniques computational simulation method was used to characterize inhibitory mechanism 7,8,4´-trihydroxyflavone on tyrosinase. 7,8,4´-Trihydroxyflavone found strongly inhibit oxidation l-DOPA by with...
Chloroquine diphosphate (CQ) was ingeniously used to take place of phosphate salt in traditional calcium coprecipitation method for pDNA transfection. With multiple roles CQ the novel Ca-CQ-pDNA complex including compaction and assistance lysosome escape, transfection efficiency significantly increased relative method. did not intercalate into DNA double helix as free did, which probably ascribed prior mixing with high concentration chloride. In order construct efficacious vector vivo gene...
Pontocerebellar hypoplasia (PCH) is a group of neurological disorders that affect the development brain, in particular, pons and cerebellum. Homozygous mutations TBC1D23 have been found recently to lead PCH; however, underlying molecular mechanisms remain unclear. Here, we show crystal structure C-terminal domain adopts Pleckstrin homology fold selectively binds phosphoinositides, PtdIns(4)P, through one surface while binding FAM21 via opposite surface. Mutation key residues or disrupts...
Rab25, a member of the Rab family small guanosine triphosphatase, was reported to have an essential role in development human epithelial ovarian cancer. The present study demonstrated that Rab25 mediated sensitivity cancer cisplatin, first‑line chemotherapeutic agent for treatment clinic. Overexpression and increased phosphoinositide 3‑kinase (PI3K)/AKT signaling were detected cisplatin‑resistant SKOV‑3 cells compared with those cisplatin‑sensitive ES‑2 cells. results indicated cisplatin...
Abstract Bone remodeling is vital to the maintenance of bone homeostasis and may lead destructive skeletal diseases once balance disrupted. Crosstalk between Wnt estrogen receptor (ER) signaling has been proposed in remodeling, but underlying mechanism remains unclear. This study was designed explore effect Wnt‐ER during osteogenic differentiation marrow stromal cells (BMSCs). Rat BMSCs were isolated identified using flow cytometry stimulated with Wnt3a. Wnt3a treatment promoted...
This study was to investigate the role of Fas in development Cisplatin-resistant ovarian cancer. On cellular level, expression significantly reduced Cisplatin resistant A2780 (A2780/CP) cells compared with cells. silence siRNA would promote tumor cell lines proliferation, facilitate cycle transition G1/S, prevent apoptosis, and migration. Expression drug resistance gene negatively correlated Fas. In nude mice metastasis model human carcinoma by subcutaneous transplantation, after Ad-Fas...
Pyroptosis is a type of programmed cell death, displaying caspase-1-dependent and pro-inflammatory features. Purinergic 2X4 (P2X4 ) receptor activation in response to high-adenosine triphosphate release can induce inflammation. Envelope glycoprotein 120 (gp120) human immunodeficiency virus 1 considered one the primary pathogens leading neuronal injury. In this study, we investigated possible role P2X4 gp120-triggered pyroptosis cultured satellite glial cells (SGCs) rat dorsal root ganglia...
The congenital disorders of glycosylation (CDG) are a family metabolic diseases in which proteins or lipids is deficient. GDP-mannose pyrophosphorylase B (GMPPB) mutations lead to CDG, characterized by neurological and muscular defects. However, the genotype-phenotype correlation remains elusive, limiting our understanding underlying mechanism development therapeutic strategy. Here, we report case an individual presenting dystrophy with cerebellar involvement, who presents two heterozygous...
Members of the Tre2-Bub2-Cdc16 (TBC) family often function to regulate membrane trafficking and control signaling transductions pathways. As a member TBC family, TBC1D23 is critical for endosome-to-Golgi cargo by serving as bridge between Golgi-bound golgin-97/245 WASH/FAM21 complex on endosomal vesicles. However, exact mechanisms which regulates transport are poorly understood. Here, we present crystal structure N-terminus (D23N), consists both rhodanese domains. We show that domain...
Human immunodeficiency virus (HIV)-infected patients have increased rates of atherosclerotic cardiovascular diseases because the highly active antiretroviral therapy (HAART) decreased morbidity and mortality disease. Endothelial dysfunction is possibly most plausible link between HIV infection related expression cell adhesion molecules such as intercellular molecule 1 (ICAM-1) vascular (VCAM-1) on endothelial cells. HIV-1 accessory protein negative regulate factor (Nef) has been shown to be...
To develop a molecular probe for MRI detection of human tumor telomerase reverse transcriptase (hTERT) mRNA expression. Uniformly phosphorothioate‐modified hTERT antisense oligonucleotide (ASON) homing was labeled with gadolinium (Gd) through the bifunctional chelator 1,4,7, 10‐tetraazacyclododecane‐N, N', N'', N'''‐tetraacetic acid (DOTA) stirred within 45 minutes at 60°C. The Gd probes were characterized in vitro . cellular uptake rate and biodistribution 99m Tc‐DOTA‐ASON measured instead...