A5038321538- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Pluripotent Stem Cells Research
- Neuroscience and Neural Engineering
- Cancer Cells and Metastasis
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Advanced Sensor and Energy Harvesting Materials
- Magnetic and Electromagnetic Effects
- Gastrointestinal motility and disorders
- MicroRNA in disease regulation
- Neonatal Respiratory Health Research
- Heat shock proteins research
- thermodynamics and calorimetric analyses
- Liver physiology and pathology
- Muscle Physiology and Disorders
- Digestive system and related health
- Cancer Research and Treatments
- Nuclear Physics and Applications
- Virus-based gene therapy research
- Advanced biosensing and bioanalysis techniques
- Characterization and Applications of Magnetic Nanoparticles
- Inhalation and Respiratory Drug Delivery
- Biomedical Ethics and Regulation
Yonsei University
2016-2024
Abstract Matrigel, a mouse tumor extracellular matrix protein mixture, is an indispensable component of most organoid tissue culture. However, it has limited the utility organoids for drug development and regenerative medicine due to its tumor-derived origin, batch-to-batch variation, high cost, safety issues. Here, we demonstrate that gastrointestinal tissue-derived hydrogels are suitable substitutes Matrigel in We found function gastric or intestinal grown comparable often superior those...
Abstract Engineered human cardiac tissues have been utilized for various biomedical applications, including drug testing, disease modeling, and regenerative medicine. However, the applications of derived from pluripotent stem cells are often limited due to their immaturity lack functionality. Therefore, in this study, we establish a perfusable culture system based on vivo-like heart microenvironments improve tissue fabrication. The integrated platform microfluidic chip three-dimensional...
Abstract Induced hepatic (iHep) cells generated by direct reprogramming have been proposed as cell sources for drug screening and regenerative medicine. However, the practical use of a 3D tissue culture comprised iHep toxicology testing has not demonstrated. In this study, vascularized liver organoid composed decellularized extracellular matrix (LEM) cultured in microfluidic system is are transfection with polymer nanoparticles plasmids expressing transcription factors. The cocultured...
Abstract Hydrogels have been applied to improve stem cell therapy and drug delivery, but current hydrogel‐based delivery methods are inefficient in clinical settings due difficulty handling treatment processes, low off‐the‐shelf availability. To overcome these limitations, an adhesive hyaluronic acid (HA) hydrogel patch is developed that acts as a ready‐to‐use tissue tape for therapeutic application. The HA patches functionalized with phenolic moieties (e.g., catechol, pyrogallol) exhibit...
Triboelectric nanogenerators (TENGs) can be an effective cell reprogramming platform for producing functional neuronal cells therapeutic applications. stimulation accelerates nonviral direct conversion of induced from fibroblasts, increases the efficiency, and induces highly matured phenotypes with improved electrophysiological functionalities. TENG devices may also used biomedical in vivo reprogramming. As a service to our authors readers, this journal provides supporting information...
Herein, we present an unconventional method for multimodal characterization of three-dimensional cardiac organoids. This can monitor and control the mechanophysiological parameters organoids within a single device. In this method, local pressure distributions human-induced pluripotent stem-cell-derived are visualized spatiotemporally by active-matrix array pressure-sensitive transistors. is integrated with electrodes formed high-resolution printing liquid metal. These liquid-metal inserted...
Measuring cellular and tissue mechanics inside intact living organisms is essential for interrogating the roles of force in physiological disease processes. Current agents studying mechanobiology intact, are limited by poor light penetration material stability. Magnetomotive ultrasound an emerging modality real-time vivo imaging mechanics. Nonetheless, it has sensitivity spatiotemporal resolution. Here we describe magneto-gas vesicles (MGVs), protein nanostructures based on gas magnetic...
Amidst the recent coronavirus disease 2019 (COVID-19) pandemic, respiratory system research has made remarkable progress, particularly focusing on infectious diseases. Lung organoid, a miniaturized structure recapitulating lung tissue, gained global attention because of its advantages over other conventional models such as two-dimensional (2D) cell and animal models. Nevertheless, organoids still face limitations concerning heterogeneity, complexity, maturity compared to native tissue. To...
Abstract Tissue engineering of skeletal muscle has been proposed as a potential regenerative treatment for extensive damage. In this regard, the highly organized structure muscles makes alignment cells especially indispensable in tissue engineering. However, achieving desired continues to prove challenging, particularly 3D engineered constructs. study, biomimetic approach generation functional fascicle‐like tissues by recapitulating muscle‐like cellular and extracellular organization, is...
Direct cardiac reprogramming has emerged as a promising therapeutic approach for regeneration. Full chemical with small molecules to generate cardiomyocytes may be more amenable than genetic clinical applications it avoids safety concerns associated manipulations. However, challenges remain regarding low conversion efficiency and incomplete cardiomyocyte maturation. Furthermore, the potential of chemically induced (CiCMs) not been investigated. Here, we report that three-dimensional...
Abstract Organoid culture is a fast‐emerging technology in the biological and biomedical research fields, garnering interest due to its unique features of self‐organization from diverse types stem cells close resemblance organs human body. This innovative vitro model system presents possibility significant advances understanding development disease mechanisms as well discovery novel diagnostics therapeutics. Here, authors review several tissue‐specific organoids discuss most recent studies...
Naturally derived nanovesicles secreted from various cell types and found in body fluids can provide effective platforms for the delivery of cargoes because their intrinsic ability to be internalized intercellular signal transmission membrane recycling. In this study, versatility bioengineered extracellular membranous as potent carriers small‐interfering RNAs (siRNAs) stem engineering vivo has been explored. Here, exosomes have engineered, one cell‐derived vesicle types, overexpress exosomal...
Direct reprogramming is an efficient strategy to produce cardiac lineage cells necessary for tissue engineering and drug testing toxicity. However, functional maturation of reprogrammed cardiomyocytes, which great importance their regenerative potential response, still remains challenging. In this study, we propose a novel electrode platform promote direct improve the functionality cells. Nonviral was improved via three-dimensional spheroid culture chemically induced cardiomyocytes exposed...
Abstract Here, a novel anticancer gene therapy with bacterial tRNase gene, colicin D or virulence associated protein C (VapC), is suggested using biodegradable polymeric nanoparticles, such as poly(β‐amino esters) (PBAEs) carriers. These genes are meticulously selected, aiming at inhibiting translation in the recipients by hydrolyzing specific tRNA species. In terms of out 9 PBAE formulations, leading polymer, (polyethylene oxide) 4 ‐bis‐amine end‐capped poly(1,4‐butanediol diacrylate‐ co...
High-throughput drug screening and testing requires artificial liver mimics to account for metabolization. In article number 1801954, Seung-Woo Cho co-workers report a platform based on vascularized, three-dimensional organoids. Resulting from the joint application of direct programming, matrix engineering represent microenvironment, microfluidics, approach is promising as patient-relevant development cell replacement therapy.
In article number 1903863, Donghoon Choi, Seung-Woo Cho, and co-workers demonstrate phenolic hyaluronic acid hydrogel patches as a ready-to-use bioinspired adhesive tissue tape for non-invasive stem cell transplantation therapeutic drug delivery. The tapes are biocompatible, adhesive, easy to handle, providing highly effective off-the-shelf therapy regenerative medicine.
Abstract Measuring cellular and tissue mechanics inside intact living organisms is essential for interrogating the roles of force in physiological disease processes, a major goal field mechanobiology. However, existing biosensors 3D mechanics, primarily based on fluorescent emissions deformable materials, are limited vivo measurement due to light penetration poor material stability intact, organisms. While magneto-motive ultrasound (MMUS), which uses superparamagnetic nanoparticles as...
On page 7365, T. Lee, S.-W. Cho, and co-workers report a novel platform based on triboelectric nanogenerator to enhance the direct generation of induced neuronal cells from skin fibroblasts through nonviral gene delivery lineage-specific transcription factor genes, both in vitro vivo. The can be an effective for reprogramming production functional therapeutic applications.
Event Abstract Back to No Records Found Login Required This action requires you be registered with Frontiers and logged in. To register or login click here. Info The Authors in Google Scholar PubMed Related Article Close top Javascript is disabled. Please enable your browser settings order see all the content on this page.
On page 5804, S.-W. Cho and co-workers propose cell-derived bioengineered extracellular membranous nanovesicles as a novel small-interfering RNA (siRNA) delivery system, explore their potencies for stem cell engineering in vivo delivery. Modification of these via overexpression various target peptides or proteins on plasma membrane significantly improves siRNA enhanced differentiation several types human cells potential therapeutic applications vivo.