A5077563164- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- vaccines and immunoinformatics approaches
- Immune cells in cancer
- Autophagy in Disease and Therapy
- Viral Infections and Immunology Research
- Cancer Research and Treatments
- Phagocytosis and Immune Regulation
- Monoclonal and Polyclonal Antibodies Research
- Cytomegalovirus and herpesvirus research
- Cytokine Signaling Pathways and Interactions
- Drug Transport and Resistance Mechanisms
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- Circular RNAs in diseases
- Bacteriophages and microbial interactions
- Inflammasome and immune disorders
Baylor College of Medicine
2022-2025
Xuzhou Medical College
2017-2024
Cancer Institute (WIA)
2022
Soochow University
2015-2017
Institute of Infection and Immunity
2014
Chinese PLA General Hospital
2012
Nanyang Technological University
1999
Ministry of Agriculture and Rural Development
1996
Vaccines have proven effective in the treatment and prevention of numerous diseases. However, traditional attenuated inactivated vaccines suffer from certain drawbacks such as complex preparation, limited efficacy, potential risks others. These limitations restrict their widespread use, especially face an increasingly diverse range With ongoing advancements genetic engineering vaccines, DNA emerged a highly promising approach both diseases acquired While several demonstrated substantial...
The chimeric antigen receptor-modified immune effector cell (CAR-T and CAR-NK) therapies are newly developed adoptive treatments of cancers. However, their therapeutic efficacy against solid tumors is limited. Combining CAR-T or CAR-NK cells with chemotherapeutic drugs to treat tumor may be a promising strategy. We an epidermal growth factor- (EGFR-) specific third-generation CAR. NK-92 were modified the CAR by lentivirus infection. killing ability CAR-modified (CAR-NK-92) renal carcinoma...
Abstract Background/Objectives Checkpoint inhibitors, which generate durable responses in many cancer patients, have revolutionized immunotherapy. However, their therapeutic efficacy is limited, and immune-related adverse events are severe, especially for monoclonal antibody treatment directed against cytotoxic T-lymphocyte–associated protein 4 (CTLA-4), plays a pivotal role preventing autoimmunity fostering anticancer immunity by interacting with the B7 proteins CD80 CD86. Small molecules...
Objective: Neutrophils are one of the most predominant infiltrating leukocytes in lung cancer tissues and associated with progression. How neutrophils promote progression, however, has not been established. Methods: Kaplan–Meier plotter online analysis tissue immunohistochemistry were used to determine relationship between overall survival patients. The effect on was determined using Transwell migration assay, a proliferation murine tumor model. Gene knockdown poly ADP-ribose polymerase...
Transforming growth factor-β (TGF-β) plays a critical role in regulating cell and differentiation. Epithelial to mesenchymal transition (EMT) induced by TGF-β promotes cancer migration, invasion, proliferation. Pirfenidone (5-methyl-1-phenyl-2(1 H)-pyridone, PFD), an approved drug for treating pulmonary renal fibrosis, is potent inhibitor found reduced incidence of lung alleviated function decline. However, whether PFD controlling progression largely unknown. In the present study, we...
Lipid nanoparticles (LNPs) for mRNA delivery have advanced significantly, but LNP-mediated DNA still faces clinical challenges. This study compared various LNP formulations delivering DNA-encoded biologics, assessing their expression efficacy and the protective immunity generated by LNP-encapsulated in different models. The formulation used Moderna's Spikevax vaccine (LNP-M) demonstrated a stable nanoparticle structure, high efficiency, low toxicity. Notably, encoding spike protein,...
Renal carcinoma presents a rapid progression in patients with high metastasis no effective therapeutic strategy. In this study, we designed folate-grafted PEI600-CyD (H1) nanoparticle-mediated DNA vaccine containing an adjuvant of absent melanoma 2 (AIM2) and tumor-specific antigen carbonic anhydrase IX (CAIX) for renal therapy. Mice bearing subcutaneous human CAIX (hCAIX)-Renca tumor were intramuscularly immunized H1-pAIM2/pCAIX, H1-pCAIX, H1-pAIM2, or Mock vaccine, respectively. The growth...
Transketolase (TKT), which is a metabolic enzyme in the nonoxidative phase of pentose phosphate pathway (PPP), plays an important role providing cancer cells with raw materials for macromolecular biosynthesis. The ectopic expression TKT hepatocellular carcinoma (HCC) was reported previously. However, initiation liver still obscure. In our previous study, we found that deficiency protects from DNA damage by increasing levels ribose 5-phosphate and nucleotides. What's more interesting reduced...
Abstract The efficacy of chimeric antigen receptor T (CAR-T) cells for solid tumors remains unsatisfactory due to the limited tumor infiltration and immunosuppressive microenvironment. To overcome these limitations, genetically engineered recombinant oncolytic adenoviruses (OAVs) that conditionally replicate in were developed modify microenvironment (TME) facilitate CAR-T-mediated eradication. Here present study, a novel OAV carrying CCL5, IL12, IFN-γ controlled by Ki67 promoter was...
p53, the most frequently mutated gene in cancer, lacks effective targeted drugs. We developed monoclonal antibodies (mAbs) that target a p53 hotspot mutation E285K without cross-reactivity with wild-type p53. They were delivered using lipid nanoparticles (LNPs) encapsulate DNA plasmids. Western blot, BLI, flow cytometry, single-cell sequencing (scRNA-seq), and other methods employed to assess function of mAbs vitro vivo. These LNP-pE285K-mAbs IgG1 format exhibited robust anti-tumor effect,...
Coxsackievirus B3 (CVB3) infection is considered as the most common cause of viral myocarditis with no available vaccine. Considering that CVB3 mainly invades through gastrointestinal mucosa, development CVB3-specific mucosal vaccine, which efficient way to induce immune responses, gains more and attention. In this study, we used absent in melanoma 2 (AIM2) a adjuvant enhance immunogenicity immunoprotection chitosan-pVP1 Mice were intranasally co-immunized 50 μg chitosan-pAIM2 equal amount...
Abstract Renal cell carcinoma ( RCC ) is a high metastasis tumour with less effective treatment available currently. Absent in melanoma 2 AIM 2) as suppressor might be used potential therapeutic target for treatment. Here, we found that expression was significantly decreased patient specimens and renal lines (786‐O OSRC ‐2). To establish safe gene delivery system, formed the nanoparticles consisting of folate grafted PEI 600‐CyD (H1) nanoparticle‐mediated (H1/ pAIM an agent. Delivery H1/...
T cells survival, proliferation, and anti-tumor response are closely linked to their mitochondrial health. Complement C1q binding protein (C1QBP) promotes fitness through regulation of metabolism morphology. However, whether C1QBP regulates cell immune function remains unclear. Our data demonstrated that knockdown induced the accumulation reactive oxygen species (ROS) loss membrane potential impair fitness. At same time, insufficiency reduced recruitment anti-apoptotic proteins, including...
Albumin-bound paclitaxel (abPTX) has been widely used in cancer treatment. However, dose-related side effects, such as myelosuppression, restrict its clinical application. Cell-based targeting drug delivery is a promising way to mitigate systematic side-effects and improve antitumoral efficacy. In this study, we demonstrated that reassembled abPTX could be engulfed by neutrophils vivo delivered tumor site, thus improving therapeutic efficacy mitigating myelosuppression. First, vitro analysis...
Targeting kinases presents a potential strategy for treating solid tumors; however, the therapeutic of vaccines targeting remains uncertain.