A5015525001- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Marine Sponges and Natural Products
- Microbial Natural Products and Biosynthesis
- Phytochemistry and Biological Activities
- Natural product bioactivities and synthesis
- Traditional and Medicinal Uses of Annonaceae
- Crystal structures of chemical compounds
- Synthesis and biological activity
- Helicobacter pylori-related gastroenterology studies
- Fungal Plant Pathogen Control
- Synthetic Organic Chemistry Methods
- Plant-derived Lignans Synthesis and Bioactivity
- Plant biochemistry and biosynthesis
- Chemical synthesis and alkaloids
- Organic Light-Emitting Diodes Research
- Fungal Biology and Applications
- Synthesis and Biological Activity
- Toxin Mechanisms and Immunotoxins
- Microbial Metabolism and Applications
- Coral and Marine Ecosystems Studies
- Plant-Microbe Interactions and Immunity
- Lanthanide and Transition Metal Complexes
- Synthesis of Organic Compounds
- Alkaloids: synthesis and pharmacology
Chinese Academy of Medical Sciences & Peking Union Medical College
2009-2025
Peking Union Medical College Hospital
2008-2025
Sun Yat-sen University
2024-2025
Systems Biology Institute
2024
Fudan University
2015-2024
Shanghai Center for Brain Science and Brain-Inspired Technology
2022-2024
Shanghai Institute for Science of Science
2024
Peking University
2014-2023
Chinese Academy of Medical Sciences Dermatology Hospital
2021-2023
State Grid Corporation of China (China)
2023
Biosynthetic pathways containing multiple core enzymes have potential to produce structurally complex natural products. Here we mined a fungal gene cluster that contains two predicted terpene cyclases (TCs) and nonribosomal peptide synthetase (NRPS). We showed the flv pathway produces flavunoidine 1, an alkaloidal terpenoid. The of 1 is tetracyclic, cage-like, oxygenated sesquiterpene connected dimethylcadaverine via C–N bond acylated with 5,5-dimethyl-l-pipecolate. roles all are established...
Acetohydroxyacid synthase (AHAS) is the first enzyme in branched-chain amino acid biosynthetic pathway and a validated target for herbicide fungicide development. Here we report harzianic (HA,
ABSTRACT This study aimed to develop and validate a nomogram based on lymphocyte subtyping clinical factors for the early rapid prediction of Intra‐abdominal candidiasis (IAC) in septic patients. A prospective cohort 633 consecutive patients diagnosed with sepsis intra‐abdominal infection (IAI) was performed. We assessed characteristics subsets at onset IAI. machine‐learning random forest model used select important variables, multivariate logistic regression analyze influencing IAC....
Liver fibrosis can progress to cirrhosis and hepatocellular carcinoma. Currently, there is no effective drug for liver fibrosis. The peptide 6 (T6) an endogenous derived from human intrauterine adhesion tissues has antifibrotic potential. Here, improve the long-term efficacy activity of T6, we conducted rational modified T6 through studying structure–activity, synthesized a series analogues. Among them, S6 S6-FA exhibited optimal antihepatic activity, had stronger long-acting effect than S6....
Eleven new metabolites, butanolides 1−6, lignan derivatives 7−9, sesquiterpene 10, and 3′,4′-seco-flavane derivative 11, have been isolated from an ethanol extract of Machilus wangchiana. Twenty known compounds, including ginkgolides A B (16 17), were also isolated. Their structures absolute configurations determined by spectroscopic chemical methods. Compounds 7, 8a, 8b, 9, (+)-guaiacin (12), meso-dihydroguaiaretic acid (13), hamabiwalactone (15) showed potent in vitro activities against...
Sixteen new neolignans and lignans (1–16), together with 12 known analogues, have been isolated from an ethanol extract of the bark Machilus robusta. Compounds 1 2 showed activity against HIV-1 replication in vitro, IC50 values 2.52 2.01 μM, respectively. At 10 6, 8, 9 reduced dl-galactosamine-induced hepatocyte (WB-F344 cells) damage, could additionally attenuate rotenone-induced PC12 cell damage. The compounds (−)-pinoresinol (17) (+)-lyoniresinol (18) were active serum deprivation induced
Antibacterial activity assessment and high performance liquid chromatography associated with nuclear magnetic resonance (HPLC/NMR) data revealed that the EtOAc extract of fermented endophytic fungus Trichoderma saturnisporum DI-IA, obtained from marine sponge Dictyonella incisa, contained conjugated olefinic metabolites antibacterial activity. Chemical examination fungal strain resulted in isolation eight new sorbicillinoid-based compounds, namely saturnispols A⁻H (1⁻8). Their structures...
Lanosterol 14α-demethylase (CYP51) is an important target in the development of antifungal drugs. The fungal-derived restricticin 1 and related molecules are only examples natural products that inhibit CYP51. Here, using colocalizations genes encoding self-resistant CYP51 as query, we identified validated biosynthetic gene cluster (BGC) 1. Additional genome mining BGCs with led to production lanomycin 2. pathways for both 2 were from fungi not known produce these compounds, highlighting...
The fungal natural product CJ-12662 is a structurally complex terpene-amino acid hybrid, and potent anthelmintic compound. biosynthetic pathway of elucidated based on metabolite analysis from heterologous expression. We demonstrate the terpene portion derived successive P450-catalyzed oxidations amorpha-4,11-diene, while three flavin-dependent enzymes are involved in morphing esterified tryptophan into chlorinated pyrrolobenzoxazine, utilizing cascaded [1,2]-Meisenheimer rearrangement.
Anti-biofilm assay guided fractionation of the marine sponge
Aromatic polyketides from marine actinomycetes have received increasing attention due to their unusual structures and potent bioactivities. Compared terrestrial counterparts, aromatic been less discovered structural biological diversities are far being fully investigated. In this study, we employed a PCR-based genome mining method discover in our bacteria collection. Five new atypical angucyclinones, fluostatins M-Q (1-5) featuring unique 6-5-6-6 ring skeleton, were one "positive"...
Thirty-one new 4H-chromene derivatives were designed and synthesized. Their structures identified with IR, 1H NMR, 13C HRMS. The crystal structure of compound 2a was determined by single-crystal X-ray diffraction. antifungal activities evaluated against Pyricularia oryzae, Erysiphe graminis, Coniella diplodiella, Pseudoperonospora cubensis, Sclerotinia sclerotiorum. These results demonstrated that most compounds exhibited remarkable inhibitory at 20 μg/mL. Compounds 4b 4c displayed excellent...
To discover new fungicides targeting succinate dehydrogenase (SDH), 36 furan/thiophene carboxamides containing 4,5-dihydropyrazole rings were designed, synthesized, and characterized. The crystal structure of compound 5l was determined with the X-ray diffraction (XRD) single crystals. antifungal activity these compounds studied against Botrytis cinerea, Pyricularia oryzae, Erysiphe graminis, Physalospora piricola, Penicillium digitatum. Bioassay results that most had obvious inhibitory at 20...
Nine minor new tirucallane (1−7) and euphane (8 9) triterpenoids including five hydroperoxides, together with 18 known compounds, have been isolated from an ethanolic extract of the roots Euphorbia micractina. Their structures absolute configurations were elucidated by spectroscopic chemical analysis. In in vitro assays, betulin (10) showed a selective cytotoxic activity against A2780 ovarian cells IC50 value 6.1 μM inhibitory protein tyrosine phosphatase 1B (PTP1B) 15.3 μM. Jolkinol B (11)...
Eleven new compounds including two sesquiterpenes with an unusual 2,2,5,9-tetramethylbicyclo[6.3.0]undecane carbon skeleton (1 and 2), five phytane-type diterpene dilactones (3−7), ent-clerodane dilactone (8), three phenylpropenol esters (9−11), together a diacylphenol (12) 38 known compounds, have been isolated from ethanolic extract of Heteroplexis micocephala. Their structures absolute configurations were elucidated by spectroscopic chemical analyses. In the in vitro assays, compound 6...