Background . Pathogenic infection in broilers has become an important issue the development of poultry industry. Xylooligosaccharides released from xylan via xylanase and fermented polysaccharide Hericium caputmedusae (FPHC) have antimicrobial potential against many pathogens. Objective We aimed to explore effects FPHC on pathogenic ( Gallus gallus domesticus ). Methods Three hundred thirty 21‐day male were assigned into four groups: control group (CG, basic diet), (XG, diet + xylanase),...

The use of bioresorbable compositions has been considered a promising therapeutic approach for treating compromised bone tissues. Gellan gum (GG) is predominant polysaccharide recognized its exceptional biocompatibility and biodegradability, facile bio-fabrication, customizable mechanical attributes, rendering it well-suited developing versatile scaffolds. On the other hand, MXene nanosheets have declared representational filler to augment osteogenic effect amend properties polymeric...

A porous carbon material was prepared from quinoa husk (QH) by carbonization and chemical activation with KOH. series of experiments, including SEM (Scanning electron microscopy), FT-IR (Fourier transform infrared), XRD (X-ray diffraction), Raman, X-ray photoelectron spectroscopy (XPS), N2 adsorption/desorption, were carried out on the produced (PC–QH). The results showed that PC–QH mainly composed activated graphite. Moreover, exhibited a high level porosity BET (the Brunauer–Emmett–Teller...

Acinetobacter baumannii is a nosocomial bacterial pathogen and responsible for wide range of diseases including pneumonia, necrotizing fasciitis, meningitis, sepsis. The enzyme 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase (encoded by aroA gene) in ESKAPE pathogens catalyzes the sixth step shikimate pathway. pathway an attractive drug targets as it present bacteria but absent humans. As EPSP essential A. growth needed during infection process, therefore was used target herein...

Abstract Saccharomyces cerevisiae -based expression systems, which rely on safe, food-grade strains, are low cost, simple to operate, and can be used for large-scale fermentation. However, levels of foreign protein by S. have limited their widespread application. The ability the endoplasmic reticulum (ER) fold process proteins is an important factor restricting proteins. In current study, effects transcription Hac1p, involved in unfolded response pathway, xylanase gene xynB from Aspergillus...

AIM:To identify the characteristics of morphology, location and collateral circulation involved in paraesophageal varices (para-EV) portal hypertension patients with 64-row multidetector computed tomography (MDCT).METHODS: Fifty-two 501 hypertensive cirrhosis accompanied esophageal were selected for MDCT examination after observation para-EV.The CT protocol included unenhanced, arterial phases a slice thickness 0.625 mm scanning field 2 cm above bifurcation to lower edge kidney.The...

Abstract To date, a large number of Bacillus species from different sources have been identified. However, there are few investigations on genome information and evolutionary insights cold environments. sp. TK-2, isolated the soil Changbai Mountain, is gram-positive bacterium with adaptation characteristics. In this study, we present annotated complete sequence TK-2. The comprised 5,286,177 bp GC content 35.88%, 5293 protein-encoding genes, 32 rRNA, 77 tRNA. Numerous genes related to were...

The aim of this study was to investigate the efficacy lesion-oriented whole-liver computed tomography (CT) perfusion for predicting transarterial chemoembolization early treatment response in patients with hepatocellular carcinoma (HCC).Volume helical shuttle CT imaging on whole liver prospectively performed 39 46 independent HCC lesions before target-selected chemoembolization. therapeutic effects were assessed: responder group included a complete and partial response, whereas nonresponder...

An organic solvent-tolerant strain producing lipase was isolated. The optimal pH and temperature were 8.0 34°C, respectively. stable at 50°C after 1 h incubation 6 to 8. BF-3 very when incubated with methanol, ethanol toluene below 30% (v/v) but stability reduced drastically above 50%. While K + , Na Mg 2+ Ba (1 mMol/L; 3 mMol/L) stimulated activity, EDTA inhibited its activity indicating that the from Bacillus cereus a metalloenzyme. Key words: Organic, solvent, tolerant, lipase, BF-3,...

Six highly pathogenic avian influenza (HPAI) H5N1 viruses (clade 2.3.4.4b) were detected in migratory birds Hubei Province November 2021. Phylogenetic analysis indicated that the study included two different reassortants between circulating Eurasia and low-pathogenic (LPAIVs). Several amino acid substitutions contributed to enhanced replication or virulence mammals observed these viruses, suggesting a potential threat of human health. IMPORTANCE Here, we obtained whole-genomes six from dead...

Lignocellulose biorefinery via continuous cell-recycle fermentation has long been recognized as a promising alternative technique for producing chemicals. ᴅ-lactic acid (D-LA) production by of corn stover Lactobacillus bulgaricus was proven to be feasible previous study. However, the phenolic compounds and high glucose content in this substrate may inhibit cell growth. The immobilization cells polymer hydrogels can protect them from toxic medium improve efficiency. Here, we studied D-LA L....

With the development of petrochemical industry, number storage tanks has continuously increased, exacerbating issue oil evaporation losses. Therefore, it is urgent to find efficient and economical measures reduce This paper establishes a diffusion model for internal floating roof (IFRTs) uses numerical simulation methods study mass fraction distribution, pressure variation patterns vapor inside at different heights. The results show that closer top tank, lower fraction, distribution almost...

The production of D-lactic acid (D-LA) from non-detoxified corn stover hydrolysate is hindered by substrate-mediated inhibition and low cell utilization times. In this study, we developed a novel temperature-sensitive hydrogel, F127-IEA, for efficient D-LA using cell-recycle batch fermentation process.

Multi-drug resistance (MDR) bacterial pathogens pose a threat to global health and warrant the discovery of new therapeutic molecules, particularly those that can neutralize their virulence stop evolution resistant mechanisms. The superbug nosocomial pathogen,

In this study, we reported the cloning and over expression of a gene coding for human collagen peptide (CP6) in Escherichia coli investigated protective effects CP6 on UVA-irradiated skin fibroblasts cells. The was highly soluble level approximately 10% total bacteria proteins. Also, performed assays with 3-(4,5-dimethylthiazol2-yl)-2,5-diphenyltetrazolium bromide (MTT), Annexin V-fluorescein isothiocyanate/propidine iodide (Annexin V-FITC/PI) terminal deoxynucleotidyl transferase dUTP nick...

Objective To establish a rapid, accurate and specific method to detect the common mycobacteria based on multiplex real-time PCR. Methods The dual priming oligonucleotide （DPO）primers TaqMan probes labeled with FAM，ROX，HEX or JOE fluoresceins at 5′ end eclipse 3′ respectively were designed 16S rRNA of mycobacteria.Both specificity sensitivity estimated PCR detecting genome DNA from 4 mycobacterial model species.Sixty eight early morning sputum specimens collected hospitalized patients...

Abstract Backgroud: Saussurea involucrata Kar. et Kir. (Compositae) (CCSauI) cells are rich in caffeoylquinic acids (CQAs), which have plasma lipid reducing properties and anti-obesity effects, although the mechanisms remain unclear. Clarify CQA’s mechanism provide new treatments for obesity. Methods: Sprague Dawley (SD) rat were fed a high-fat diet (HFD), then CQAs was intragastric administrated (0.1, 0.5 or 1 mg/mL). Rats randomly divided into five groups (n=30): NC, MC, TPL (0.1 mg/mL),...