Hyperglycemia upregulates thioredoxin interacting protein (TXNIP) expression, which in turn induces ROS production, inflammatory and fibrotic responses the diabetic kidney. Dysregulation of autophagy contributes to development nephropathy. However, interaction TXNIP with autophagy/mitophagy nephropathy is unknown. In this study, streptozotocin-induced rats were given DNAzyme or scrambled for 12 weeks respectively. Fibrotic markers, mitochondrial function reactive oxygen species (mtROS)...

The Ca(2+)-activated K(+) channel KCa3.1 mediates cellular signaling processes associated with dysfunction of vasculature. However, the role in diabetic nephropathy is unknown. We sought to assess whether development renal fibrosis two animal models nephropathy. Wild-type and KCa3.1(-/-) mice, secondly eNOS(-/-) had diabetes induced streptozotocin then were treated with/without a selective inhibitor (TRAM34). Our results show that albumin-to-creatinine ratio significantly decreased mice...

Abstract Autophagy is emerging as an important pathway in many diseases including diabetic nephropathy. It acknowledged that oxidative stress plays a critical role autophagy dysfunction and nephropathy KCa3.1 blockade ameliorates renal fibrosis through inhibiting TGF-β1 signaling pathway. To identify the of dysfunctional tubular nephropathy, human proximal cells (HK2) transfected with scrambled or siRNAs were exposed to for 48 h, then autophagosome formation, marker LC3, molecules PI3K, Akt...

Circular RNAs (circRNAs) are highly enriched in the central nervous system and have been implicated neurodegenerative diseases. However, whether how circRNAs contribute to pathological processes induced by traumatic brain injury (TBI) has not fully elucidated.We conducted a high-throughput RNA sequencing screen for well-conserved, differentially expressed cortex of rats subjected experimental TBI. METTL9 (circMETTL9) was ultimately identified as upregulated post-TBI further characterized...

Background and Objective Sodium glucose cotransporter 2 (SGLT2) is the main luminal transporter in kidney. SGLT2 inhibition results glycosuria improved glycaemic control. Drugs inhibiting this have recently been approved for clinical use suggested to potential renoprotective benefits by limiting glycotoxicity proximal tubule. We aimed determine of empagliflozin, an inhibitor, independent its lowering effect. Research Design Methods induced diabetes using a low dose streptozotocin protocol...

It is well-known that all progressive chronic kidney disease (CKD) pathologically characterized by tubulointerstitial fibrosis process. Multiple studies have shown the critical role of inflammation and in development CKD. Hence strategies target inflammatory fibrotic signaling pathways may provide promising opportunities to protect against renal fibrosis. Metformin has been used as first-line glucose-lowering agent treat patients with type 2 diabetes mellitus (T2DM) for over 50 years....

Autoimmune thyroiditis (AIT), encompassing Hashimoto's (HT), is a prevalent chronic autoimmune disorder characterized by lymphocytic infiltration and the presence of anti-thyroid antibodies. It primary cause hypothyroidism affects women more frequently than men. Nearly 95% individuals with HT exhibit thyroid peroxidase antibodies or thyroglobulin Dietary factors, including vitamins trace elements such as zinc, play significant role in health; yet, clinical guidelines lack explicit dietary...

To evaluate the effect and factors associated with reactivation of retinopathy prematurity (ROP) after intravitreal conbercept or aflibercept. We retrospectively reviewed medical records 176 eyes diagnosed ROP treated anti-VEGF therapy between January 2018 September 2022. The rate complications were assessed during follow-up period. aflibercept analyzed on basis clinical retinal parameters. Reactivation occurred in 10 (13.9%) 13 (12.5%) (P = 0.79). interval injection was significantly longer...

Thoracic aortic aneurysm (TAA) is a life-threatening peripheral vascular disease with complex pathogenesis. Altered mitochondrial function in smooth muscle cells has been implicated TAA development. However, the link between functional status and immune cell behavior patients remains largely unexplored. In this study, we analyzed several bulk RNA-seq snRNA-seq datasets of from NCBI-GEO Genome Sequence Archive database, identifying differentially expressed mitochondrial-related genes...

Fibroblast activation plays a critical role in diabetic nephropathy (DN). The Ca2+-activated K+ channel KCa3.1 mediates cellular proliferation of many cell types including fibroblasts. has been reported to be potential molecular target for pharmacological intervention diverse array clinical conditions. However, the myofibroblasts DN is unknown. These studies assessed effect blockade on renal injury experimental diabetes. As TGF-β1 central fibroblasts interstitial fibrosis, human primary were...

Diabetic kidney disease (DKD) is a life-limiting condition characterized by progressive and irreversible loss of renal function. Currently, the estimated glomerular filtration rate (eGFR) albuminuria are used as key markers to define DKD. However, they may not accurately indicate degree dysfunction injury. Current therapeutic approaches for DKD, including attainment blood pressure goals, optimal control glucose lipid levels, use agents block renin-angiotensin-aldosterone system (RAAS) can...

Abstract Receptor-interacting protein kinase-3 (RIPK3) is a multifunctional regulator of cell death and inflammation. RIPK3 controls cellular signalling through the formation domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome, which recognised to mediate renal fibrogenesis. The role in diabetic kidney disease (DKD) induced fibrosis has not been previously determined. To define action development disease, wild-type (WT), -/- endothelium-derived nitric oxide synthase...

The mammalian cyclin kinase subunit (Cks) family has two members, Cks1 and Cks2, which were identified based on the protein sequence homology to yeast Cks. Overexpression of Cks2 been reported be associated with high aggressiveness a poor prognosis in various malignancies, including gastric, breast prostate carcinomas. Yet, whether are overexpressed hepatocellular carcinoma (HCC) remains uncharacterized.To investigate overexpression Cks is clinically relevant HCC, expression patterns HCC...

Citrus aurantium L. var. amara Engl. (CAVA) was traditionally used as a digestant or expectorant in China. Crude polyphenols (CAVAP-W) extracted from blossoms of CAVA were mainly composed eriocitrin/neoeriocitrin, rhoifolin, hesperidin, naringin, rutin, veronicastroside, neohesperidin, and hesperetin by LC-MS analysis. CAVAP-W showed significant anticomplement anti-inflammatory effects. Due to the close relationship between activity, effect further investigated results that significantly...

Progressive tubulointerstitial fibrosis has been recognized as a common pathological process that leads to the progression of all chronic kidney disease (CKD). Innovative strategies are needed both prevent and treat CKD. Inflammatory fibrotic signaling pathways play central roles in CKD regardless aetiology. Hence, targeting inflammatory responses holds promise limit renal fibrosis. Metformin most prescribed glucose-lowering medicine worldwide, its potential for many other therapeutic...

Aberrant activation of inflammation and excess accumulation lipids play pivotal roles in atherosclerosis (AS) progression.

SGLT-2 inhibitors, such as empagliflozin, have been shown to reduce the occurrence of cardiovascular events and delay progression atherosclerosis. However, its role in atherosclerotic calcification remains unclear. In this research, ApoE-/- mice were fed with western diet empagliflozin was added drinking water for 24 weeks. Empagliflozin treatment significantly alleviated arterial assessed by alizarin red von kossa staining aortic roots reduced lipid levels, while had little effect on body...