A5069265987- SARS-CoV-2 and COVID-19 Research
- Computational Drug Discovery Methods
- Pharmacological Effects of Natural Compounds
- Traditional Chinese Medicine Studies
- Bacterial Infections and Vaccines
- Atherosclerosis and Cardiovascular Diseases
- Traditional Chinese Medicine Analysis
- Dermatology and Skin Diseases
- Psoriasis: Treatment and Pathogenesis
- CAR-T cell therapy research
- Skin Protection and Aging
- Monoclonal and Polyclonal Antibodies Research
- Phytochemicals and Antioxidant Activities
- Advanced Proteomics Techniques and Applications
- Natural product bioactivities and synthesis
- Cholesterol and Lipid Metabolism
- vaccines and immunoinformatics approaches
- Anesthesia and Sedative Agents
- Neurological Disease Mechanisms and Treatments
- Corneal Surgery and Treatments
- Genomics, phytochemicals, and oxidative stress
- Chromatography in Natural Products
- Crystallography and molecular interactions
- Cytokine Signaling Pathways and Interactions
- Corneal surgery and disorders
Guangdong Provincial Hospital of Traditional Chinese Medicine
2007-2024
Guangzhou University of Chinese Medicine
2012-2024
Lanzhou University
2024
Lanzhou University Second Hospital
2024
Guangdong Academy of Medical Sciences
2018-2023
ShanghaiTech University
2019-2023
National Center for Advancing Translational Sciences
2020-2022
National Institutes of Health
2014-2022
Army Medical University
2016-2019
Southwest Hospital
2016-2019
While vaccine development will hopefully quell the global pandemic of COVID-19 caused by SARS-CoV-2, small molecule drugs that can effectively control SARS-CoV-2 infection are urgently needed. Here, inhibitors spike (S) mediated cell entry were identified in a high throughput screen an approved library with SARS-S and MERS-S pseudotyped particle assays. We discovered six compounds (cepharanthine, abemaciclib, osimertinib, trimipramine, colforsin, ingenol) to be broad spectrum for...
The first step of SARS-CoV-2 infection is binding the spike protein's receptor domain to host cell's ACE2 on plasma membrane. Here, we have generated a versatile imaging probe using recombinant Spike conjugated fluorescent quantum dots (QDs). This capable engaging in energy transfer quenching with ACE2-conjugated gold nanoparticles enable monitoring event solution. Neutralizing antibodies and human blocked quenching, demonstrating specific interaction. In cells transfected ACE2-GFP, observed...
Understanding the SARS-CoV-2 virus' pathways of infection, virus–host–protein interactions, and mechanisms virus-induced cytopathic effects will greatly aid in discovery design new therapeutics to treat COVID-19. Chloroquine hydroxychloroquine, extensively explored as clinical agents for COVID-19, have multiple cellular including alkalizing lysosomes blocking autophagy well exhibiting dose-limiting toxicities patients. Therefore, we evaluated additional lysosomotropic compounds identify an...
Essential oil has been popularly used as an alternative for the treatment of inflammation. The bioactivities essential from blossoms Citrus aurantium L. var. amara Engl (CAVAO) showed greater anti-inflammation potential than that antioxidant, anticancer, and 3T3-L1 proliferation inhibition. CAVAO (250 μg/mL) significantly inhibited production nitric oxide (NO) (99.54 ± 2.81%), interleukin-6 (IL-6) (98.11 1.62%), tumor necrosis factor-α (TNF-α) (41.84 1.52%), interleukin-1β (IL-1β) (56.09...
Abstract Purpose: Immunogenicity derived from the murine scFv, a major molecular compomemt of chimeric antigen receptors (CARs), may limit persistence CAR T cells, resulting in tumor relapse patients complete remission (CR). In this study, we developed humanized anti-CD19 scFv CAR-T (hCAR-T) to treat with relapsed/refractory acute lymphoblastic leukemia (r/r ALL). Patients and Methods: one-arm, open-labeled infused cells modified hCAR r/r ALL. were evaluated long-term follow-up for response...
Application of retinol (Vitamin A, VA) in skincare is limited for instability, poor water solubility, and skin intolerance that combats aging. We employed computer-aided virtual screening cell experiments with transcriptomics, thereby unveiling the comprehensive gene expression regulation pathway photoaging HaCaT treated ferulic acid (FA) synergizing VA. Through network pharmacology analysis, combined use VA FA exhibited highly correlated cross-targets aging acting on EGFR, PTPN1, ESR2,...
This study assessed the prevalence, species and subtypes of Cryptosporidium in goats from Guangdong Province, Hubei Shandong Shanghai City China. Six hundred four fecal samples were collected twelve goat farms, overall infection rate was 11.4% (69/604). Goats infected with found eleven farms across provincial areas, ranged 2.9% (1/35) to 25.0% (9/36). Three identified. xiaoi (45/69, 65.2%) dominant species, followed by C. parvum (14/69, 20.3%) ubiquitum (10/69, 14.5%). The spp. varied host...
Psoriasis is a T cells mediated chronic skin inflammation in which helper (Th) play critical roles its pathogenesis. Taxifolin (TXL) has been discovered to exert various pharmacological activities. In this study, we wished observe whether TXL had potential activities on psoriasis, and how it works. We found that can inhibit LPS-induced abnormal proliferation Hacat cell line, ant also significantly alleviate the IMQ-induced psoriasis BALB/c mice, comparing with control group. Although no...
Identification of anti-SARS-CoV-2 compounds through traditional high-throughput screening (HTS) assays is limited by high costs and low hit rates. To address these challenges, we developed machine learning models to identify acting via inhibition the entry SARS-CoV-2 into human host cells or 3-chymotrypsin-like (3CL) protease. The optimal classification achieved good performance with area under receiver operating characteristic curve (AUC-ROC) values >0.78. Experimental validation showed...
Phloretin and isoniazid formed new cocrystal the structure, solubility antitumor activities were study.
The majority of neutralizing antibodies (NAbs) against SARS-CoV-2 recognize the receptor-binding domain (RBD) spike (S) protein. As an escaping strategy, RBD virus is highly variable, evolving mutations to thwart a natural immune response or vaccination. Targeting non-RBD regions S protein thus provides viable alternative generating potential, robust NAbs. Using pre-pandemic combinatorial antibody library 1011, through alternate negative and positive screening 11 non-RBD-targeting are...
The discovery of new therapeutic drugs with the efficacious and safe ability to prevent epidermal hyperplasia is extremely urgent for psoriasis. Cryptotanshinone (CTS), an active component isolated from root Salvia miltiorrhiza Bunge, has been reported have antibacterial antitumor effects. However, its effects on psoriasis not reported. Here, we investigated CTS imiquimod (IMQ)-induced psoriatic-like skin model explored underlying mechanisms. Our results revealed that effectively alleviates...
The pmel-1 T cell receptor transgenic mouse has been extensively employed as an ideal model system to study the mechanisms of tumor immunology, CD8+ differentiation, autoimmunity and adoptive immunotherapy. 'zygosity' transgene affects expression levels may compromise optimal breeding scheme design. However, integration sites for have remained uncharacterized. This is also true many other commonly used mice created before modern era rapid inexpensive next-generation sequencing. Here, we show...
The IL-17-producing CD4+ T cell and γδT cells play critical roles in the pathogenesis of psoriasis (PS). PSORI-CM02 is a representative herbal formula for treatment PS South China. It was confirmed to improve without obvious side effects clinic. Here we sought clarify whether how regulates differentiation functions IMQ-induced psoriasis-like BALB/c mouse model. Mice pre-treated 3 days with significantly alleviated skin inflammation, as reduced PASI score classic psoriatic characteristics...
Dietary consumption of flavonoids correlated positively with lower risk cardiovascular disease. However, the precise roles from blossoms Citrus aurantium Linn variant amara Engl (CAVA) in atherosclerosis (AS) are still poorly understood. This study aimed to find novel flavonoid-type skeletons protection against AS. Total (CAVAF), homoeriodictyol (HE) and hesperetin-7-O-β-d-glucopyranoside (HG) were isolated Engl. by chromatography. Their suppressive effects on lipopolysaccharide...
Aberrant activation of inflammation and excess accumulation lipids play pivotal roles in atherosclerosis (AS) progression.
Abstract The outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2) has emphasized the urgency to develop effective therapeutics. Drug repurposing screening is regarded as one most practical and rapid approaches for discovery such 3C like protease (3CL pro ), or main (M ) SARS-CoV-2 a valid drug target it specific viral enzyme plays an essential role in replication. We performed quantitative high throughput (qHTS) 10,755 compounds...
A novel water soluble, phosphate-based enrichable, retro-Michael addition-driven MS-cleavable and multi-targeting cross-linker was developed.
Salidroside (SAL) is the main component of Renus Rhodiola, Studies have shown that it has anti-inflammation, reduces oxidative stress and anti-aging effect. However, its efficacy mechanism protecting skin barrier function not been studied. This study demonstrated whether how SAL alleviate UVB-induced damage repair barrier. UVB was used to induce photodamage in HaCaT cell line vitro. MTT assay detect protective effect on induced light cells, flow cytometry apoptosis ROS level related kits...
Psoriasis is a refractory skin disorder, and usually requires lifetime control. Traditional Chinese medicine (TCM) effective safe for this disease. However, the cellular molecular mechanisms of TCM remedies psoriasis are still not fully understood. contains numerous natural products. Natural products have historically been invaluable as resource therapeutic agents. Yet, there no integrated information about active compounds psoriasis.We use systems pharmacology methods to develop Database...