A5070080934- Mosquito-borne diseases and control
- COVID-19 epidemiological studies
- Maternal and Neonatal Healthcare
- Glioma Diagnosis and Treatment
- Pituitary Gland Disorders and Treatments
- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Bone Tumor Diagnosis and Treatments
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Molecular Biology Techniques and Applications
- Antibiotic Resistance in Bacteria
- Nail Diseases and Treatments
- Healthcare Regulation
- COVID-19 Pandemic Impacts
- Genomics and Phylogenetic Studies
- Science and Education Research
- Women's cancer prevention and management
- Bacteriophages and microbial interactions
- Cytomegalovirus and herpesvirus research
- Adrenal and Paraganglionic Tumors
- Phytoplasmas and Hemiptera pathogens
- Biosensors and Analytical Detection
- Healthcare during COVID-19 Pandemic
Instituto Adolfo Lutz
2017-2024
Hospital do Servidor Público Estadual
2024
Instituto de Assistência Médica ao Servidor Público Estadual
2024
Universidade de São Paulo
2017-2023
Wellborn Road Veterinary Medical Center
2020
Departamento de Epidemiología
2020
Universidade Federal de São Paulo
2018
Cases of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in Manaus, Brazil, resurged late 2020 despite previously high levels infection. Genome sequencing viruses sampled Manaus between November and January 2021 revealed the emergence circulation a novel SARS-CoV-2 variant concern. Lineage P.1 acquired 17 mutations, including trio spike protein (K417T, E484K, N501Y) associated with increased binding to human ACE2 (angiotensin-converting enzyme 2) receptor. Molecular...
Abstract Cases of SARS-CoV-2 infection in Manaus, Brazil, resurged late 2020, despite high levels previous there. Through genome sequencing viruses sampled Manaus between November 2020 and January 2021, we identified the emergence circulation a novel variant concern, lineage P.1, that acquired 17 mutations, including trio spike protein (K417T, E484K N501Y) associated with increased binding to human ACE2 receptor. Molecular clock analysis shows P.1 occurred around early was preceded by period...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Gamma variant of concern has spread rapidly across Brazil since late 2020, causing substantial infection and death waves. Here we used individual-level patient records after hospitalization with suspected or confirmed disease 2019 (COVID-19) between 20 January 2020 26 July 2021 to document temporary, sweeping shocks in hospital fatality rates that followed the 14 state capitals, during which typically more than half...
A major outbreak of yellow fever (YF) occurred in Brazil during 2016-2018. Epizootics New World nonhuman primates are sentinel events for YF virus circulation. However, genus-specific susceptibilities and suitability surveillance remain poorly understood. We obtained compared epidemiologic, histopathologic, immunohistochemical, molecular results from 93 human 1,752 primate cases submitted the recent (2017), with support Brazilian National Surveillance Program. detected heterogeneous...
ACTH-dependent hypercortisolism caused by a pituitary adenoma [Cushing's disease (CD)] is the most common cause of endogenous Cushing's syndrome. CD often associated with several morbidities, including hypertension, diabetes, osteoporosis/bone fractures, secondary infections, and increased cardiovascular mortality. While majority (≈80%) corticotrophinomas visible on magnetic resonance imaging are microadenomas (MICs, <10 mm diameter), some tumors macroadenomas (MACs, ≥10 mm) growth potential...
For the preservation of tissue samples, formalin fixation followed by paraffin embedding (FFPE) has been method choice for decades, mainly because it maintains morphologic characteristics original particularly preserved, as well its genetic material. FFPE cells can be used to perform molecular tests, such conventional (c) or quantitative (q) reverse transcriptase polymerase chain reaction (RT-PCR), in retrospective investigations. However, extracting RNA from archived tissues is a...
Abstract The SARS-CoV-2 Gamma variant spread rapidly across Brazil, causing substantial infection and death waves. We use individual-level patient records following hospitalisation with suspected or confirmed COVID-19 to document the extensive shocks in hospital fatality rates that followed Gamma’s 14 state capitals, which more than half of hospitalised patients died over sustained time periods. show fluctuations in-hospital also existed prior detection, were largely transient after...
Background Sporotrichosis is an emerging zoonotic mycosis that presents as a cutaneous lymphatic or disseminated disease, caused by fungi from the Sporothrix schenkii ( S ) clinical clade. Its importance growing, primarily due to outbreak occurred in Brazil, affecting mainly cats and people. Objectives In diagnosis often made using cultures, which allows genus identification sufficient growth perform molecular biology testing. Despite its advantages, fungal cultures are slow develop can...
New-generation sequencing (NGS) techniques have brought the opportunity for genomic monitoring of several microorganisms potentially relevant to public health. The establishment different methods with mechanisms provides a wide choice, taking into account aspects. With that in mind, present aim study was compare basic metrics could impact genotyping by nanopores from Oxford Nanopore Technologies and synthesis Illumina clinical samples positive Chikungunya (CHIKV). Among studied, running...
Introduction. Brazil was one of the most affected countries by COVID-19 pandemic. Instituto Adolfo Lutz (IAL) is reference laboratory for in São Paulo, populous state Brazil. In April 2020, a secondary diagnostic pole named IAL-2 created to enhance IAL's capacity diagnosis.Hypothesis/Gap Statement. Public health laboratories must be prepared rapidly respond emerging epidemics or pandemics.Aim. To describe design and correlate results RT-qPCR tests with data on suspected cases SARS-CoV-2...
It has been previously demonstrated that the hemodynamic effect induced by angiotensin II (AII) in liver was completely abolished losartan while glucose release partially affected losartan. Angiotensin type 1 (AT1) and adrenergic (∝1- β-) receptors (AR) belong to G-proteins superfamily, which signaling promote glycogen breakdown release. Interactive relationship between AR AT1-R shown after blockade of these with specific antagonists. The isolated perfused rat used study metabolic responses...
The cell block (CB) technique has allowed easy obtainment of samples such as cellular and culture suspensions, to perform specific molecular tests immunohistochemistry in situ hybridization. It been improved along time, accuracy, quality the diagnoses, however, cost a commercial gel matrix for preparation CB is high not suitable depending on situation. objective this study test agarose an alternative Aspergillus fumigatus’ CB.
Formalin-fixed paraffin-embedded (FFPE) tissues are an important source for investigation of dengue virus (DENV) infection, particularly when blood or fresh frozen (FF) samples unavailable. Histopathologic features and immunohistochemistry may have poor sensitivity serotype determination is not always possible. Viral RNA genome detection tests faster considered the most sensitive technique this kind analysis, however, use molecular methods applied to FFPE still limited. The authors a...
Introdução: A actinomicose é uma infecção bacteriana rara, com incidência anual de cerca 1/300.000pessoas.Acomete tecidos após a inoculação microrganismos na pele ou mucosas em locais pequenos traumas.Caracteriza-se pela ocorrência tumoração, associada à formação fístulas.Objetivo: Relatar caso sugestivo paciente lesão ulcerativa região do mento.Relato caso: Paciente sexo feminino, 40 anos, residente da cidade Sorocaba (SP).Foi encaminhada para o Complexo Hospitalar por nodular crostas...

 OBJECTIVE To evaluate the performance of post mortem laboratory analysis in identifying causes hemorrhagic fever and/or neuroinvasive disease deaths by arbovirus infection. METHODS Retrospective cross-sectional study based on differential and final outcome obtained patients whose samples underwent testing for arboviruses at Pathology Center Adolfo Lutz Institute, São Paulo, Brazil. RESULTS Of 1355 adults clinically diagnosed with disease, most commonly attributed cause death common...
Introdução: A paracoccidioidomicose (PCM) é uma micose sistêmica, causada pelo gênero Paracoccidioides spp.Foi inserida entre as doenças negligenciadas de significativo impacto na saúde pública.As manifestações clínicas variam acordo com a imunidade do indivíduo, apresentando-se forma regressiva, progressiva (aguda\crônica) e residual.O diagnóstico definitivo necessita avaliação clínica especializada, pois dependente da correlação dos sintomas identificação das estruturas patognomônicas...