A5049922792- Antibiotic Resistance in Bacteria
- Antibiotic Use and Resistance
- Nosocomial Infections in ICU
- Antibiotics Pharmacokinetics and Efficacy
- Sepsis Diagnosis and Treatment
- COVID-19 Clinical Research Studies
- Enterobacteriaceae and Cronobacter Research
- Neutropenia and Cancer Infections
- SARS-CoV-2 and COVID-19 Research
- Pharmaceutical Practices and Patient Outcomes
- Bacterial Identification and Susceptibility Testing
- SARS-CoV-2 detection and testing
- Urinary Tract Infections Management
- Antimicrobial Resistance in Staphylococcus
- Maternal and Neonatal Healthcare
- Healthcare Regulation
- Bacterial biofilms and quorum sensing
- Leprosy Research and Treatment
- Streptococcal Infections and Treatments
- Viral Infections and Outbreaks Research
- Infectious Diseases and Tuberculosis
- Antifungal resistance and susceptibility
- Acute Myeloid Leukemia Research
- Infection Control in Healthcare
- COVID-19 and healthcare impacts
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2017-2025
Fleury S.A. (Brazil)
2025
Universidade de São Paulo
2012-2024
Fundação Faculdade de Medicina
2023
Biology of Infection
2023
Increased resistance to polymyxin in Klebsiella pneumoniae (ColRKP) has been observed. Molecular epidemiology, as well the clinical impact of these difficult treat pathogens need be better characterized. We present outcomes 28 patients infected by ColRKP a tertiary hospital. Isolates with MIC >2 Vitek 2 were confirmed microdilution broth test. Polymerase chain reaction (PCR) was performed for blaKPC, blaNDM, blaOXA-48 and blamcr-1 genes isolates, Whole Genome Sequencing (WGS) six isolates....
Abstract Carbapenem-resistant Enterobacteriaceae (CRE) colonization is common in hospital patients admitted to intensive care units (ICU) from the emergency department. We evaluated effect of previous hospitalization department on CRE at ICU admission. Our case–control study included 103 cases and 201 controls; were colonized by admission controls not colonized. Risk factors stay, use carbapenem, Simplified Acute Physiology Score, upper digestive endoscopy, transfer another hospital. found...
Abstract Background Bacterial resistance to carbapenems, poses a global health challenge, with Klebsiella pneumoniae and Pseudomonas aeruginosa as significant contributors. Ceftazidime-avibactam (CZA), introduced in Brazil June 2018, offers new treatment option for multi-resistant infections. However, increasing both this drug carbapenems raises concerns. Our goal is evaluate the impact of CZA introduction on patterns, metallo-betalactamases (MBL) prevalence Brazil, focusing K. P....
Abstract Background In 2019, an estimated 1.27 million deaths were attributed to bacterial antimicrobial resistance (AR) globally. During the Coronavirus Disease 2019 (COVID-19) Pandemic, additional strain was placed on healthcare systems that may have led further increases in AR. We evaluated AR prevalence before and after onset of COVID-19 6 facilities (HCF) South America. Methods conducted ecological evaluation HCF-wide 4 private 2 public HCFs Argentina, Brazil, Chile; per country....
SARS-CoV-2 infection has a wide spectrum of presentations, from asymptomatic to pneumonia and sepsis. Risk scores have been used as triggers for protocols that combine several interventions early management This study tested the accuracy score SIRS, qSOFA, NEWS in predicting outcomes, including mortality bacterial infection, patients admitted emergency department (ED) during COVID-19 pandemic. We described 2,473 cases ED largest referral hospital severe Brazil qSOFA showed poor performance...
Introduction—The dynamics of SARS-CoV-2 shedding and replication in humans remain incompletely understood. Methods—We analyzed from multiple sites individuals with an acute COVID-19 infection by weekly sampling for five weeks 98 immunocompetent 25 immunosuppressed individuals. Samples culture supernatants were tested via RT-PCR to determine viral clearance rates vitro replication. Results—A total 2447 clinical specimens evaluated, including 557 nasopharyngeal swabs, 527 saliva samples, 464...
This study aimed to provide further insight into the evolutionary dynamics of SARS-CoV-2 by analyzing case a 40-year-old man who had previously undergone autologous hematopoietic stem cell transplantation due diffuse large B-cell lymphoma. He developed persistent infection lasting at least 218 days and did not manifest humoral immune response virus during this follow-up period. Whole-genome sequencing viral cultures confirmed with replication-positive that genetic variation for 196 after...
Purpose. Carbapenem resistance in P. aeruginosa is increasing worldwide. In Brazil, SPM-1 the main carbapenemase identified. Little known about virulence factor clones. Methodolgy. We describe a carbapenem-resistant bloodstream infection (CRPa-BSI) outbreak bone marrow transplant Unit (BMT). Twenty-nine CRPa-BSI cases were compared to 58 controls. Microbiological characteristics of isolates, such as sensitivity, gene PCR for aeruginosa, and PFGE are described, well whole-genome sequence...
The objective was to evaluate the performance of surveillance cultures at various body sites for Staphylococcus aureus colonization in pregnant women and newborns (NB) factors associated with nasal colonization. For NB, 4 were evaluated: nares, oropharynx, perineum, umbilical stump (birth, third day, weekly). women, during labor: anterior anus, oropharynx. Nasally colonized patients compared only extranasally. Colonization 53% 392 (methicillin-resistant S. [MRSA]: 4%) 47% 382 NB (MRSA: 9%)....
Abstract Background High rates of antibiotic use (AU) among inpatients with coronavirus disease 2019 (COVID-19) despite low bacterial coinfection and secondary infection have been reported. We evaluated the impact COVID-19 pandemic on AU in healthcare facilities (HCFs) South America. Methods conducted an ecologic evaluation inpatient adult acute care wards 2 HCFs each Argentina, Brazil, Chile. The for intravenous antibiotics were calculated as defined daily dose per 1000 patient-days, using...
Hematopoietic stem cell transplant (HSCT) recipients are at -increased risk for severe COVID-19. The aim of this study was to evaluate the burden COVID-19 in a cohort HSCT recipients. This retrospective evaluated adult hospitalized diagnosed with two large hospitals São Paulo, Brazil post-HSCT, from January 2020 June 2022. primary outcome all-cause mortality. Of 49 cases, 63.2% were male median age 47 years. Allogeneic-HSCT (51.2%) and autologous-HSCT (48.9%) patients included. time...
Overcrowded emergency departments (EDs) may increase the risk of carbapenem-resistant Enterobacterales (CRE) transmission.We conducted a quasi-experimental study divided into 2 phases (baseline and intervention) to investigate impact an intervention on acquisition rate identify factors for CRE colonization in ED tertiary academic hospital Brazil. In both phases, we did universal screening with rapid molecular test (blaKPC, blaNDM, blaOXA48, blaOXA23, blaIMP) culture. At baseline, results...
In the last decade, non-multiresistant methicillin-resistant Staphylococcus aureus (NM-MRSA) has been described as an important agent in bloodstream infections our hospital.This prospective cohort study, conducted from February 2009 through January 2010 neonatal unit, evaluated 403 newborns (NB), their 382 mothers and 148 health care workers (HCW).Approximately 217 NB (54%), 187 (48%) 87 HCW (59%) were colonized by S. (SA). MRSA colonization was greater among (15%) than (4.7%) (3.4%)....
Abstract This report describes a persistent SARS-CoV-2 infection of at least 218 days in male, his 40’s who had undergone prior autologous hematopoietic stem cell transplant due to diffuse large B-cell lymphoma. He did not manifest humoral immune response the virus. Whole-genome sequencing and viral cultures confirmed continual with replication-positive virus that genetic variation for 196 following symptom onset.
Surveillance strategies to detect colonization are an important tool prevent and control the spread of microorganisms in hematopoietic stem cell transplant (HSCT) units. The aim this study was evaluate routine surveillance cultures for screening infection by carbapenem-resistant Enterobacteriaceae (CRE), Pseudomonas aeruginosa (CRPa), vancomycin-resistant enterococci (VRE). were collected (1,323 samples) from 200 patients admitted HSCT unit over one year; swabs taken on admission then...
Background: Healthcare-associated infections (HAI) substantially increase hospital costs and lead to poor patient outcomes, particularly when caused by multidrug-resistant organisms (MDRO). To decrease MDRO transmission, isolation of colonized or infected patients in single rooms is recommended. However, single-patient are expensive build often unavailable resource-limited hospitals. In 2023, an intensive care unit (ICU) at a large Brazilian tertiary relocated from space with open floor plan...
Abstract Background Our aim in this retrospective cohort study was to assess the impact on mortality of empirical use polymyxin as therapy for carbapenem-resistant gram-negative bacteria (CR-GNB) septic patients. The performed at a tertiary academic hospital Brazil, from January 2018 2020, pre–coronavirus disease 2019 period. Methods We included 203 patients with suspected sepsis. first doses antibiotics were prescribed “sepsis antibiotic kit”, which contained selection drugs, including...